A re-investigation of the bloom-forming unarmored dinoflagellate Karenia longicanalis (syn. Karenia umbella) from Chinese coastal waters
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A re-investigation of the bloom-forming unarmored dinoflagellate Karenia longicanalis (syn. Karenia umbella) from Chinese coastal waters. / Wang, Jianyan; Cen, Jingyi; Li, Si; Lü, Songhui; Moestrup, Øjvind; Chan, Kin Ka; Jiang, Tao; Lei, Xiangdong.
In: Journal of Oceanology and Limnology, Vol. 36, No. 6, 2018, p. 2202-2215.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - A re-investigation of the bloom-forming unarmored dinoflagellate Karenia longicanalis (syn. Karenia umbella) from Chinese coastal waters
AU - Wang, Jianyan
AU - Cen, Jingyi
AU - Li, Si
AU - Lü, Songhui
AU - Moestrup, Øjvind
AU - Chan, Kin Ka
AU - Jiang, Tao
AU - Lei, Xiangdong
PY - 2018
Y1 - 2018
N2 - The dinoflagellate genus Karenia is known for recurrent harmful blooms worldwide. However, species diversity of the genus is generally overlooked owing to the difficulty of identifying small unarmored dinoflagellates. We have established four clonal cultures of Karenia longicanalis isolated from the type locality, Hong Kong harbor (strain HK01) and other three locations along the Chinese coasts (strains YB01, DT01, and NJ01). The morphology of the strain was studied by light and scanning electron microscopy (LM and SEM) and the pigment composition analyzed by high-performance liquid chromatography. We provide the first molecular data of K. longicanalis based on the large subunit (LSU) rRNA gene sequence and internal transcribed spacer (ITS). The four strains showed identical LSU rDNA sequences with a similarity of 99.4% to the holotype of Karenia umbella (strain KUTN05) from Australia. In the ITS phylogeny, the sequence of K. umbella branched between the Chinese strains of K. longicanalis. A careful comparison of the morphology of K. longicanalis and K. umbella reveals the similarity in the diagnostic characters. Differences may appear due to the sample treatment for SEM. We conclude that K. umbella is a junior synonym of K. longicanalis.
AB - The dinoflagellate genus Karenia is known for recurrent harmful blooms worldwide. However, species diversity of the genus is generally overlooked owing to the difficulty of identifying small unarmored dinoflagellates. We have established four clonal cultures of Karenia longicanalis isolated from the type locality, Hong Kong harbor (strain HK01) and other three locations along the Chinese coasts (strains YB01, DT01, and NJ01). The morphology of the strain was studied by light and scanning electron microscopy (LM and SEM) and the pigment composition analyzed by high-performance liquid chromatography. We provide the first molecular data of K. longicanalis based on the large subunit (LSU) rRNA gene sequence and internal transcribed spacer (ITS). The four strains showed identical LSU rDNA sequences with a similarity of 99.4% to the holotype of Karenia umbella (strain KUTN05) from Australia. In the ITS phylogeny, the sequence of K. umbella branched between the Chinese strains of K. longicanalis. A careful comparison of the morphology of K. longicanalis and K. umbella reveals the similarity in the diagnostic characters. Differences may appear due to the sample treatment for SEM. We conclude that K. umbella is a junior synonym of K. longicanalis.
KW - harmful algal blooms (HABs)
KW - internal transcribed spacer (ITS)
KW - large subunit (LSU) rRNA
KW - morphology
KW - phylogeny
KW - phytoplankton
U2 - 10.1007/s00343-019-7191-4
DO - 10.1007/s00343-019-7191-4
M3 - Journal article
AN - SCOPUS:85058981498
VL - 36
SP - 2202
EP - 2215
JO - Journal of Oceanology and Limnology
JF - Journal of Oceanology and Limnology
SN - 2096-5508
IS - 6
ER -
ID: 211854061