The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans. / Klasson, Lisa; Westberg, Joakim; Sapountzis, Panagiotis; Näslund, Kristina; Lutnaes, Ylva; Darby, Alistair C; Veneti, Zoe; Chen, Lanming; Braig, Henk R; Garrett, Roger; Bourtzis, Kostas; Andersson, Siv G E.

In: Proceedings of the National Academy of Science of the United States of America, Vol. 106, No. 14, 2009, p. 5725-5730.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Klasson, L, Westberg, J, Sapountzis, P, Näslund, K, Lutnaes, Y, Darby, AC, Veneti, Z, Chen, L, Braig, HR, Garrett, R, Bourtzis, K & Andersson, SGE 2009, 'The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans', Proceedings of the National Academy of Science of the United States of America, vol. 106, no. 14, pp. 5725-5730. https://doi.org/10.1073/pnas.0810753106

APA

Klasson, L., Westberg, J., Sapountzis, P., Näslund, K., Lutnaes, Y., Darby, A. C., Veneti, Z., Chen, L., Braig, H. R., Garrett, R., Bourtzis, K., & Andersson, S. G. E. (2009). The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans. Proceedings of the National Academy of Science of the United States of America, 106(14), 5725-5730. https://doi.org/10.1073/pnas.0810753106

Vancouver

Klasson L, Westberg J, Sapountzis P, Näslund K, Lutnaes Y, Darby AC et al. The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans. Proceedings of the National Academy of Science of the United States of America. 2009;106(14):5725-5730. https://doi.org/10.1073/pnas.0810753106

Author

Klasson, Lisa ; Westberg, Joakim ; Sapountzis, Panagiotis ; Näslund, Kristina ; Lutnaes, Ylva ; Darby, Alistair C ; Veneti, Zoe ; Chen, Lanming ; Braig, Henk R ; Garrett, Roger ; Bourtzis, Kostas ; Andersson, Siv G E. / The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans. In: Proceedings of the National Academy of Science of the United States of America. 2009 ; Vol. 106, No. 14. pp. 5725-5730.

Bibtex

@article{b622f8c01f5511de9f0a000ea68e967b,
title = "The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans",
abstract = "The obligate intracellular bacterium Wolbachia pipientis infects around 20% of all insect species. It is maternally inherited and induces reproductive alterations of insect populations by male killing, feminization, parthenogenesis, or cytoplasmic incompatibility. Here, we present the 1,445,873-bp genome of W. pipientis strain wRi that induces very strong cytoplasmic incompatibility in its natural host Drosophila simulans. A comparison with the previously sequenced genome of W. pipientis strain wMel from Drosophila melanogaster identified 35 breakpoints associated with mobile elements and repeated sequences that are stable in Drosophila lines transinfected with wRi. Additionally, 450 genes with orthologs in wRi and wMel were sequenced from the W. pipientis strain wUni, responsible for the induction of parthenogenesis in the parasitoid wasp Muscidifurax uniraptor. The comparison of these A-group Wolbachia strains uncovered the most highly recombining intracellular bacterial genomes known to date. This was manifested in a 500-fold variation in sequence divergences at synonymous sites, with different genes and gene segments supporting different strain relationships. The substitution-frequency profile resembled that of Neisseria meningitidis, which is characterized by rampant intraspecies recombination, rather than that of Rickettsia, where genes mostly diverge by nucleotide substitutions. The data further revealed diversification of ankyrin repeat genes by short tandem duplications and provided examples of horizontal gene transfer across A- and B-group strains that infect D. simulans. These results suggest that the transmission dynamics of Wolbachia and the opportunity for coinfections have created a freely recombining intracellular bacterial community with mosaic genomes.",
author = "Lisa Klasson and Joakim Westberg and Panagiotis Sapountzis and Kristina N{\"a}slund and Ylva Lutnaes and Darby, {Alistair C} and Zoe Veneti and Lanming Chen and Braig, {Henk R} and Roger Garrett and Kostas Bourtzis and Andersson, {Siv G E}",
note = "Keywords: horizontal transfer recombination ankyrin repeat gene genome evolution insect symbiosis",
year = "2009",
doi = "10.1073/pnas.0810753106",
language = "English",
volume = "106",
pages = "5725--5730",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "14",

}

RIS

TY - JOUR

T1 - The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans

AU - Klasson, Lisa

AU - Westberg, Joakim

AU - Sapountzis, Panagiotis

AU - Näslund, Kristina

AU - Lutnaes, Ylva

AU - Darby, Alistair C

AU - Veneti, Zoe

AU - Chen, Lanming

AU - Braig, Henk R

AU - Garrett, Roger

AU - Bourtzis, Kostas

AU - Andersson, Siv G E

N1 - Keywords: horizontal transfer recombination ankyrin repeat gene genome evolution insect symbiosis

PY - 2009

Y1 - 2009

N2 - The obligate intracellular bacterium Wolbachia pipientis infects around 20% of all insect species. It is maternally inherited and induces reproductive alterations of insect populations by male killing, feminization, parthenogenesis, or cytoplasmic incompatibility. Here, we present the 1,445,873-bp genome of W. pipientis strain wRi that induces very strong cytoplasmic incompatibility in its natural host Drosophila simulans. A comparison with the previously sequenced genome of W. pipientis strain wMel from Drosophila melanogaster identified 35 breakpoints associated with mobile elements and repeated sequences that are stable in Drosophila lines transinfected with wRi. Additionally, 450 genes with orthologs in wRi and wMel were sequenced from the W. pipientis strain wUni, responsible for the induction of parthenogenesis in the parasitoid wasp Muscidifurax uniraptor. The comparison of these A-group Wolbachia strains uncovered the most highly recombining intracellular bacterial genomes known to date. This was manifested in a 500-fold variation in sequence divergences at synonymous sites, with different genes and gene segments supporting different strain relationships. The substitution-frequency profile resembled that of Neisseria meningitidis, which is characterized by rampant intraspecies recombination, rather than that of Rickettsia, where genes mostly diverge by nucleotide substitutions. The data further revealed diversification of ankyrin repeat genes by short tandem duplications and provided examples of horizontal gene transfer across A- and B-group strains that infect D. simulans. These results suggest that the transmission dynamics of Wolbachia and the opportunity for coinfections have created a freely recombining intracellular bacterial community with mosaic genomes.

AB - The obligate intracellular bacterium Wolbachia pipientis infects around 20% of all insect species. It is maternally inherited and induces reproductive alterations of insect populations by male killing, feminization, parthenogenesis, or cytoplasmic incompatibility. Here, we present the 1,445,873-bp genome of W. pipientis strain wRi that induces very strong cytoplasmic incompatibility in its natural host Drosophila simulans. A comparison with the previously sequenced genome of W. pipientis strain wMel from Drosophila melanogaster identified 35 breakpoints associated with mobile elements and repeated sequences that are stable in Drosophila lines transinfected with wRi. Additionally, 450 genes with orthologs in wRi and wMel were sequenced from the W. pipientis strain wUni, responsible for the induction of parthenogenesis in the parasitoid wasp Muscidifurax uniraptor. The comparison of these A-group Wolbachia strains uncovered the most highly recombining intracellular bacterial genomes known to date. This was manifested in a 500-fold variation in sequence divergences at synonymous sites, with different genes and gene segments supporting different strain relationships. The substitution-frequency profile resembled that of Neisseria meningitidis, which is characterized by rampant intraspecies recombination, rather than that of Rickettsia, where genes mostly diverge by nucleotide substitutions. The data further revealed diversification of ankyrin repeat genes by short tandem duplications and provided examples of horizontal gene transfer across A- and B-group strains that infect D. simulans. These results suggest that the transmission dynamics of Wolbachia and the opportunity for coinfections have created a freely recombining intracellular bacterial community with mosaic genomes.

U2 - 10.1073/pnas.0810753106

DO - 10.1073/pnas.0810753106

M3 - Journal article

C2 - 19307581

VL - 106

SP - 5725

EP - 5730

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 14

ER -

ID: 11709863