Type III CRISPR-Cas System: Introduction And Its Application for Genetic Manipulations
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Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes provide adaptive immunity against invasion of foreign nucleic acids in archaea and bacteria. The system functions in three distinct stages: adaptation, biogenesis, and interference. CRISPR-Cas systems are currently classified into at least five different types, each with a signature protein among which Type III systems exhibit a dual DNA/RNA interference activity. Structures of a few Type III surveillance complexes have been determined: they are composed of several different subunits and exhibit striking architectural similarities to Type I surveillance complexes. Here, we review the genetic, biochemical, and structural studies concerning CRISPR-Cas Type III systems and discuss their application for genetic manipulations, including genome engineering and gene silencing.
Original language | English |
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Journal | Current Issues in Molecular Biology |
Volume | 26 |
Pages (from-to) | 1-14 |
Number of pages | 14 |
ISSN | 1467-3037 |
DOIs | |
Publication status | Published - 1 Jan 2018 |
ID: 209706207