A Bacterial Genome and Culture Collection of Gut Microbial in Weanling Piglet
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The microbiota hosted in the pig gastrointestinal tract are important to health of this biomedical model. However, the individual species and functional repertoires that make up the pig gut microbiome remain largely undefined. Here we comprehensively investigated the genomes and functions of the piglet gut microbiome using culture-based and metagenomics approaches. A collection included 266 cultured genomes and 482 metagenome- assembled genomes (MAGs) that were clustered to 428 species across 10 phyla was established. Among these clustered species, 333 genomes represent potential new species. Less matches between cultured genomes and MAGs revealed a substantial bias for the acquisition of reference genomes by the two strategies. Glycoside hydrolases was the dominant category of carbohydrate-active enzymes. Four-hundred forty-five secondary metabolite biosynthetic genes were predicted from 292 genomes with bacteriocin being the most. Pan genome analysis of Limosilactobacillus reuteri uncover the biosynthesis of reuterin was strain-specific and the production was experimentally determined. This study provides a comprehensive view of the microbiome composition and the function landscape of the gut of weanling piglets and a valuable bacterial resource for further experimentations.
Originalsprog | Engelsk |
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Artikelnummer | e02417-21 |
Tidsskrift | Microbiology Spectrum |
Vol/bind | 10 |
Udgave nummer | 1 |
Antal sider | 13 |
ISSN | 2165-0497 |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
This work was supported by grants from National Natural Science Foundation of China (32002287), Guangzhou Municipal Science and Technology Bureau (201804010338, 202102080151), Science and Technology Program of Guangdong Province (2014B020201002, 2016A030303034), the Key Laboratory of Modern Biological Seed Industry in South China, Ministry of Agriculture and Rural Affairs (2105-000000-20-03-457451), the Special Rural Revitalization Funds of Guangdong Province (2021KJ382), and Guangdong Provincial Key Laboratory of Genome Read and Write (No. 2017B030301011).
Publisher Copyright:
© 2022 Dong et al.
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