A CD146 FACS Protocol Enriches for Luminal Keratin 14/19 Double Positive Human Breast Progenitors
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A CD146 FACS Protocol Enriches for Luminal Keratin 14/19 Double Positive Human Breast Progenitors. / Ísberg, Ólöf Gerdur; Kim, Jiyoung; Fridriksdottir, Agla J.; Morsing, Mikkel; Timmermans-Wielenga, Vera; Rønnov-Jessen, Lone; Petersen, Ole W.; Villadsen, René.
I: Scientific Reports, Bind 9, 14843, 2019.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - A CD146 FACS Protocol Enriches for Luminal Keratin 14/19 Double Positive Human Breast Progenitors
AU - Ísberg, Ólöf Gerdur
AU - Kim, Jiyoung
AU - Fridriksdottir, Agla J.
AU - Morsing, Mikkel
AU - Timmermans-Wielenga, Vera
AU - Rønnov-Jessen, Lone
AU - Petersen, Ole W.
AU - Villadsen, René
N1 - Publisher Correction: A CD146 FACS Protocol Enriches for Luminal Keratin 14/19 Double Positive Human Breast Progenitors DOI: 10.1038/s41598-020-60887-6
PY - 2019
Y1 - 2019
N2 - Human breast cancer is believed to arise in luminal progenitors within the normal breast. A subset of these are double positive (DP) for basal and luminal keratins and localizes to a putative stem cell zone within ducts. We here present a new protocol based on a combination of CD146 with CD117 and CD326 which provides an up to thirty fold enrichment of the DP cells. We show by expression profiling, colony formation, and morphogenesis that CD146high/CD117high/CD326high DP cells belong to a luminal progenitor compartment. While these DP cells are located quite uniformly in ducts, with age a variant type of DP (vDP) cells, which is mainly CD146-negative, accumulates in lobules. Intriguingly, in specimens with BRCA1 mutations known to predispose for cancer, higher frequencies of lobular vDP cells are observed. We propose that vDP cells are strong candidates for tracing the cellular origin of breast cancer.
AB - Human breast cancer is believed to arise in luminal progenitors within the normal breast. A subset of these are double positive (DP) for basal and luminal keratins and localizes to a putative stem cell zone within ducts. We here present a new protocol based on a combination of CD146 with CD117 and CD326 which provides an up to thirty fold enrichment of the DP cells. We show by expression profiling, colony formation, and morphogenesis that CD146high/CD117high/CD326high DP cells belong to a luminal progenitor compartment. While these DP cells are located quite uniformly in ducts, with age a variant type of DP (vDP) cells, which is mainly CD146-negative, accumulates in lobules. Intriguingly, in specimens with BRCA1 mutations known to predispose for cancer, higher frequencies of lobular vDP cells are observed. We propose that vDP cells are strong candidates for tracing the cellular origin of breast cancer.
UR - https://doi.org/10.1038/s41598-020-60887-6
U2 - 10.1038/s41598-019-50903-9
DO - 10.1038/s41598-019-50903-9
M3 - Journal article
C2 - 31619692
VL - 9
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 14843
ER -
ID: 229318804