A history of the MetaSUB consortium: Tracking urban microbes around the globe
Publikation: Bidrag til tidsskrift › Kommentar/debat › Forskning › fagfællebedømt
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The MetaSUB Consortium, founded in 2015, is a global consortium with an interdisciplinary team of clinicians, scientists, bioinformaticians, engineers, and designers, with members from more than 100 countries across the globe. This network has continually collected samples from urban and rural sites including subways and transit systems, sewage systems, hospitals, and other environmental sampling. These collections have been ongoing since 2015 and have continued when possible, even throughout the COVID-19 pandemic. The consortium has optimized their workflow for the collection, isolation, and sequencing of DNA and RNA collected from these various sites and processing them for metagenomics analysis, including the identification of SARS-CoV-2 and its variants. Here, the Consortium describes its foundations, and its ongoing work to expand on this network and to focus its scope on the mapping, annotation, and prediction of emerging pathogens, mapping microbial evolution and antibiotic resistance, and the discovery of novel organisms and biosynthetic gene clusters.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | 104993 |
| Tidsskrift | iScience |
| Vol/bind | 25 |
| Udgave nummer | 11 |
| Antal sider | 12 |
| ISSN | 2589-0042 |
| DOI |
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| Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
We thank all members of the MetaSUB International Consortium for their continued contributions and support for this study. We'd also like to acknowledge all organizations and grants for their support, including but not limited to the WorldQuant Foundation, the Bill and Melinda Gates Foundation, the Alfred P. Sloan Foundation, and the Rockefeller Foundation. Editorially, Dr. Abul Arif and Dr. Michelle Muzzio at iScience facilitated the transcription of the meeting of the co-authors as well as worked with the editing and flow of the Backstory. The following authors contributed to the writing and editing of the manuscript: A.F. A.K. B.T.T. C.D. C.E.M. C.O. C.G. D.B. D.H. E.D. E.E. E.A. G.G. G.I. H.S. J.W. K.U. K.A.R. L.S. M.B. M.O. M.M.Z. N.H.H.C. O.O. P.P.L. P.T. S.R. S.B. S.P. T.S. U.S. X.R. and Z.H.S. Review of the manuscript and final edits were made by B.T.T. and K.A.R. Overall supervision and approval of the manuscript, C.E.M.
Funding Information:
What helped us overcome some of the most significant challenges is that we successfully established philanthropic and corporate partnerships, which are integral to enabling this science. The Alfred P. Sloan foundation first expressed interest in expanding our work beyond New York, helping us kick the entire project off. The Bill and Melinda Gates Foundation funding was also a big help, which led to other funding and recent support from the Rockefeller Foundation. Illumina donated some flow cells and other reagents, and Promega, New England Biolabs, and QIAGEN donated or discounted reagents and support. Arbor Biotechnologies helped with computational analysis and our annual meetings. We also received donations of DNA/RNA shield buffer to transport samples from Zymo Research, and they have also helped with centralized extraction and uniform processing methods. Informatics support from Amazon and Twist Bioscience donation of controls also helped a lot. These supporters are a demonstration of strong belief in the projects that we are engaged in.
Publisher Copyright:
© 2022 The Authors
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