A sparsomycin-resistant mutant of Halobacterium salinarium lacks a modification at nucleotide U2603 in the peptidyl transferase centre of 23 S rRNA

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Standard

A sparsomycin-resistant mutant of Halobacterium salinarium lacks a modification at nucleotide U2603 in the peptidyl transferase centre of 23 S rRNA. / Lázaro, Ester; Rodriguez-Fonseca, Cristina; Porse, Bo; Ureña, Dionisio; Garrett, Roger A.; Ballesta, Juan P.G.

I: Journal of Molecular Biology, Bind 261, Nr. 2, 16.08.1996, s. 231-238.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Lázaro, E, Rodriguez-Fonseca, C, Porse, B, Ureña, D, Garrett, RA & Ballesta, JPG 1996, 'A sparsomycin-resistant mutant of Halobacterium salinarium lacks a modification at nucleotide U2603 in the peptidyl transferase centre of 23 S rRNA', Journal of Molecular Biology, bind 261, nr. 2, s. 231-238. https://doi.org/10.1006/jmbi.1996.0455

APA

Lázaro, E., Rodriguez-Fonseca, C., Porse, B., Ureña, D., Garrett, R. A., & Ballesta, J. P. G. (1996). A sparsomycin-resistant mutant of Halobacterium salinarium lacks a modification at nucleotide U2603 in the peptidyl transferase centre of 23 S rRNA. Journal of Molecular Biology, 261(2), 231-238. https://doi.org/10.1006/jmbi.1996.0455

Vancouver

Lázaro E, Rodriguez-Fonseca C, Porse B, Ureña D, Garrett RA, Ballesta JPG. A sparsomycin-resistant mutant of Halobacterium salinarium lacks a modification at nucleotide U2603 in the peptidyl transferase centre of 23 S rRNA. Journal of Molecular Biology. 1996 aug. 16;261(2):231-238. https://doi.org/10.1006/jmbi.1996.0455

Author

Lázaro, Ester ; Rodriguez-Fonseca, Cristina ; Porse, Bo ; Ureña, Dionisio ; Garrett, Roger A. ; Ballesta, Juan P.G. / A sparsomycin-resistant mutant of Halobacterium salinarium lacks a modification at nucleotide U2603 in the peptidyl transferase centre of 23 S rRNA. I: Journal of Molecular Biology. 1996 ; Bind 261, Nr. 2. s. 231-238.

Bibtex

@article{d16b10f3dead43738b37865b593f23b4,
title = "A sparsomycin-resistant mutant of Halobacterium salinarium lacks a modification at nucleotide U2603 in the peptidyl transferase centre of 23 S rRNA",
abstract = "Sparsomycin, a broad-spectrum antibiotic, acts at the peptidyl transferase centre of the ribosome, stabilizing peptidyl-tRNA binding at the P-site and weakening ternary complex binding. A sparsomycin-resistant mutant was isolated for the archaeon Halobacterium salinarium and shown to lack a post-transcriptional modification of U2603 (Escherichia coli numbering U2584), which is a universally conserved uridine base located within the peptidyl transferase loop of 23 S rRNA. This mutant also exhibited altered sensitivities to the peptidyl transferase antibiotics anisomycin, chloramphenicol and puromycin. Several lines of evidence indicate that the unmodified uridine base lies within the P-substrate site of the peptidyl transferase centre.",
keywords = "23 S rRNA, Drug-resistant mutant, Peptidyl transferase, Post-transcriptional modification, Sparsomycin",
author = "Ester L{\'a}zaro and Cristina Rodriguez-Fonseca and Bo Porse and Dionisio Ure{\~n}a and Garrett, {Roger A.} and Ballesta, {Juan P.G.}",
year = "1996",
month = aug,
day = "16",
doi = "10.1006/jmbi.1996.0455",
language = "English",
volume = "261",
pages = "231--238",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - A sparsomycin-resistant mutant of Halobacterium salinarium lacks a modification at nucleotide U2603 in the peptidyl transferase centre of 23 S rRNA

AU - Lázaro, Ester

AU - Rodriguez-Fonseca, Cristina

AU - Porse, Bo

AU - Ureña, Dionisio

AU - Garrett, Roger A.

AU - Ballesta, Juan P.G.

PY - 1996/8/16

Y1 - 1996/8/16

N2 - Sparsomycin, a broad-spectrum antibiotic, acts at the peptidyl transferase centre of the ribosome, stabilizing peptidyl-tRNA binding at the P-site and weakening ternary complex binding. A sparsomycin-resistant mutant was isolated for the archaeon Halobacterium salinarium and shown to lack a post-transcriptional modification of U2603 (Escherichia coli numbering U2584), which is a universally conserved uridine base located within the peptidyl transferase loop of 23 S rRNA. This mutant also exhibited altered sensitivities to the peptidyl transferase antibiotics anisomycin, chloramphenicol and puromycin. Several lines of evidence indicate that the unmodified uridine base lies within the P-substrate site of the peptidyl transferase centre.

AB - Sparsomycin, a broad-spectrum antibiotic, acts at the peptidyl transferase centre of the ribosome, stabilizing peptidyl-tRNA binding at the P-site and weakening ternary complex binding. A sparsomycin-resistant mutant was isolated for the archaeon Halobacterium salinarium and shown to lack a post-transcriptional modification of U2603 (Escherichia coli numbering U2584), which is a universally conserved uridine base located within the peptidyl transferase loop of 23 S rRNA. This mutant also exhibited altered sensitivities to the peptidyl transferase antibiotics anisomycin, chloramphenicol and puromycin. Several lines of evidence indicate that the unmodified uridine base lies within the P-substrate site of the peptidyl transferase centre.

KW - 23 S rRNA

KW - Drug-resistant mutant

KW - Peptidyl transferase

KW - Post-transcriptional modification

KW - Sparsomycin

UR - http://www.scopus.com/inward/record.url?scp=0030590237&partnerID=8YFLogxK

U2 - 10.1006/jmbi.1996.0455

DO - 10.1006/jmbi.1996.0455

M3 - Journal article

C2 - 8757290

AN - SCOPUS:0030590237

VL - 261

SP - 231

EP - 238

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 2

ER -

ID: 199465012