Anti-CRISPR Proteins in Archaea

Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt

Standard

Anti-CRISPR Proteins in Archaea. / Peng, Xu; Mayo-Muñoz, David; Bhoobalan-Chitty, Yuvaraj; Martínez-Álvarez, Laura.

I: Trends in Microbiology, Bind 28, Nr. 11, 2020, s. 913-921.

Publikation: Bidrag til tidsskrift › Review › Forskning › fagfællebedømt

Harvard

Peng, X, Mayo-Muñoz, D, Bhoobalan-Chitty, Y & Martínez-Álvarez, L 2020, 'Anti-CRISPR Proteins in Archaea', Trends in Microbiology, bind 28, nr. 11, s. 913-921. https://doi.org/10.1016/j.tim.2020.05.007

APA

Peng, X., Mayo-Muñoz, D., Bhoobalan-Chitty, Y., & Martínez-Álvarez, L. (2020). Anti-CRISPR Proteins in Archaea. Trends in Microbiology, 28(11), 913-921. https://doi.org/10.1016/j.tim.2020.05.007

Vancouver

Peng X, Mayo-Muñoz D, Bhoobalan-Chitty Y, Martínez-Álvarez L. Anti-CRISPR Proteins in Archaea. Trends in Microbiology. 2020;28(11):913-921. https://doi.org/10.1016/j.tim.2020.05.007

Author

Peng, Xu ; Mayo-Muñoz, David ; Bhoobalan-Chitty, Yuvaraj ; Martínez-Álvarez, Laura. / Anti-CRISPR Proteins in Archaea. I: Trends in Microbiology. 2020 ; Bind 28, Nr. 11. s. 913-921.

Bibtex

@article{b696cb6e01c74b59a4e8787390bfb3f6,
title = "Anti-CRISPR Proteins in Archaea",
abstract = "Anti-CRISPR (Acr) proteins are natural inhibitors of CRISPR-Cas immune systems. To date, Acrs inhibiting types I, II, III, V, and VI CRISPR-Cas systems have been characterized. While most known Acrs are derived from bacterial phages and prophages, very few have been characterized in the domain Archaea, despite the nearly ubiquitous presence of CRISPR-Cas in archaeal cells. Here we summarize the discovery and characterization of the archaeal Acrs with the representatives encoded by a model archaeal virus, Sulfolobus islandicus rod-shaped virus 2 (SIRV2). AcrID1 inhibits subtype I-D CRISPR-Cas immunity through direct interaction with the large subunit Cas10d of the effector complex, and AcrIIIB1 inhibits subtype III-B CRISPR-Cas immunity through a mechanism interfering with middle/late gene targeting. Future development of efficient screening methods will be key to uncovering the diversity of archaeal Acrs.",
keywords = "AcrID1, AcrIIIB1, anti-CRISPR (Acr), Archaea, archaeal virus, Sulfolobus rod-shaped virus 2 (SIRV2)",
author = "Xu Peng and David Mayo-Mu{\~n}oz and Yuvaraj Bhoobalan-Chitty and Laura Mart{\'i}nez-{\'A}lvarez",
year = "2020",
doi = "10.1016/j.tim.2020.05.007",
language = "English",
volume = "28",
pages = "913--921",
journal = "Trends in Microbiology",
issn = "0966-842X",
publisher = "Elsevier Ltd. * Trends Journals",
number = "11",

}

RIS

TY - JOUR

T1 - Anti-CRISPR Proteins in Archaea

AU - Peng, Xu

AU - Mayo-Muñoz, David

AU - Bhoobalan-Chitty, Yuvaraj

AU - Martínez-Álvarez, Laura

PY - 2020

Y1 - 2020

N2 - Anti-CRISPR (Acr) proteins are natural inhibitors of CRISPR-Cas immune systems. To date, Acrs inhibiting types I, II, III, V, and VI CRISPR-Cas systems have been characterized. While most known Acrs are derived from bacterial phages and prophages, very few have been characterized in the domain Archaea, despite the nearly ubiquitous presence of CRISPR-Cas in archaeal cells. Here we summarize the discovery and characterization of the archaeal Acrs with the representatives encoded by a model archaeal virus, Sulfolobus islandicus rod-shaped virus 2 (SIRV2). AcrID1 inhibits subtype I-D CRISPR-Cas immunity through direct interaction with the large subunit Cas10d of the effector complex, and AcrIIIB1 inhibits subtype III-B CRISPR-Cas immunity through a mechanism interfering with middle/late gene targeting. Future development of efficient screening methods will be key to uncovering the diversity of archaeal Acrs.

AB - Anti-CRISPR (Acr) proteins are natural inhibitors of CRISPR-Cas immune systems. To date, Acrs inhibiting types I, II, III, V, and VI CRISPR-Cas systems have been characterized. While most known Acrs are derived from bacterial phages and prophages, very few have been characterized in the domain Archaea, despite the nearly ubiquitous presence of CRISPR-Cas in archaeal cells. Here we summarize the discovery and characterization of the archaeal Acrs with the representatives encoded by a model archaeal virus, Sulfolobus islandicus rod-shaped virus 2 (SIRV2). AcrID1 inhibits subtype I-D CRISPR-Cas immunity through direct interaction with the large subunit Cas10d of the effector complex, and AcrIIIB1 inhibits subtype III-B CRISPR-Cas immunity through a mechanism interfering with middle/late gene targeting. Future development of efficient screening methods will be key to uncovering the diversity of archaeal Acrs.

KW - AcrID1

KW - AcrIIIB1

KW - anti-CRISPR (Acr)

KW - Archaea

KW - archaeal virus

KW - Sulfolobus rod-shaped virus 2 (SIRV2)

U2 - 10.1016/j.tim.2020.05.007

DO - 10.1016/j.tim.2020.05.007

M3 - Review

C2 - 32499102

AN - SCOPUS:85085644355

VL - 28

SP - 913

EP - 921

JO - Trends in Microbiology

JF - Trends in Microbiology

SN - 0966-842X

IS - 11

ER -

ID: 243152527