Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria. / Lima, Stella T.; Fallon, Timothy R.; Cordoza, Jennifer L.; Chekan, Jonathan R.; Delbaje, Endrews; Hopiavuori, Austin R.; Alvarenga, Danillo O.; Wood, Steffaney M.; Luhavaya, Hanna; Baumgartner, Jackson T.; Dörr, Felipe A.; Etchegaray, Augusto; Pinto, Ernani; McKinnie, Shaun M. K.; Fiore, Marli F.; Moore, Bradley S.
I: Journal of the American Chemical Society, Bind 144, Nr. 21, 2022, s. 9372-9379.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria
AU - Lima, Stella T.
AU - Fallon, Timothy R.
AU - Cordoza, Jennifer L.
AU - Chekan, Jonathan R.
AU - Delbaje, Endrews
AU - Hopiavuori, Austin R.
AU - Alvarenga, Danillo O.
AU - Wood, Steffaney M.
AU - Luhavaya, Hanna
AU - Baumgartner, Jackson T.
AU - Dörr, Felipe A.
AU - Etchegaray, Augusto
AU - Pinto, Ernani
AU - McKinnie, Shaun M. K.
AU - Fiore, Marli F.
AU - Moore, Bradley S.
PY - 2022
Y1 - 2022
N2 - Harmful cyanobacterial blooms (cyanoHABs) cause recurrent toxic events in global watersheds. Although public health agencies monitor the causal toxins of most cyanoHABs and scientists in the field continue developing precise detection and prediction tools, the potent anticholinesterase neurotoxin, guanitoxin, is not presently environmentally monitored. This is largely due to its incompatibility with widely employed analytical methods and instability in the environment, despite guanitoxin being among the most lethal cyanotoxins. Here, we describe the guanitoxin biosynthesis gene cluster and its rigorously characterized nine-step metabolic pathway from l-arginine in the cyanobacterium Sphaerospermopsis torques-reginae ITEP-024. Through environmental sequencing data sets, guanitoxin ( gnt) biosynthetic genes are repeatedly detected and expressed in municipal freshwater bodies that have undergone past toxic events. Knowledge of the genetic basis of guanitoxin biosynthesis now allows for environmental, biosynthetic gene monitoring to establish the global scope of this neurotoxic organophosphate.
AB - Harmful cyanobacterial blooms (cyanoHABs) cause recurrent toxic events in global watersheds. Although public health agencies monitor the causal toxins of most cyanoHABs and scientists in the field continue developing precise detection and prediction tools, the potent anticholinesterase neurotoxin, guanitoxin, is not presently environmentally monitored. This is largely due to its incompatibility with widely employed analytical methods and instability in the environment, despite guanitoxin being among the most lethal cyanotoxins. Here, we describe the guanitoxin biosynthesis gene cluster and its rigorously characterized nine-step metabolic pathway from l-arginine in the cyanobacterium Sphaerospermopsis torques-reginae ITEP-024. Through environmental sequencing data sets, guanitoxin ( gnt) biosynthetic genes are repeatedly detected and expressed in municipal freshwater bodies that have undergone past toxic events. Knowledge of the genetic basis of guanitoxin biosynthesis now allows for environmental, biosynthetic gene monitoring to establish the global scope of this neurotoxic organophosphate.
U2 - 10.1021/jacs.2c01424
DO - 10.1021/jacs.2c01424
M3 - Journal article
C2 - 35583956
VL - 144
SP - 9372
EP - 9379
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
SN - 0002-7863
IS - 21
ER -
ID: 308331737