Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
The pbp gene (renamed dacC), identified by the Bacillus subtilis genome sequencing project, encodes a putative 491-residue protein with sequence homology to low-molecular-weight penicillin-binding proteins. Use of a transcriptional dacC-lacZ fusion revealed that dacC expression (i) is initiated at the end of stationary phase; (ii) depends strongly on transcription factor sigmaH; and (iii) appears to be initiated from a promoter located immediately upstream of yoxA, a gene of unknown function located upstream of dacC on the B. subtilis chromosome. A B. subtilis dacC insertional mutant grew and sporulated identically to wild-type cells, and dacC and wild-type spores had the same heat resistance, cortex structure, and germination and outgrowth kinetics. Expression of dacC in Escherichia coli showed that this gene encodes an approximately 59-kDa membrane-associated penicillin-binding protein which is highly toxic when overexpressed.
Udgivelsesdato: 1998-Sep
Udgivelsesdato: 1998-Sep
Originalsprog | Engelsk |
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Tidsskrift | Journal of Bacteriology |
Vol/bind | 180 |
Udgave nummer | 18 |
Sider (fra-til) | 4967-73 |
Antal sider | 6 |
ISSN | 0021-9193 |
Status | Udgivet - 1998 |
ID: 2830738