Congeneric temperate orchids recruit similar—yet differentially abundant—endophytic bacterial communities that are uncoupled from soil, but linked to host phenology and population size
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Besides the beneficial plant-fungus symbiosis in mycorrhizal plants, bacteria also enhance plant fitness via tripartite interactions. While bacterial associations are presumably just as important for the obligate mycorrhizal family Orchidaceae, little is known about orchid associating bacteria (OAB).
Methods
We examined the OAB communities of two, congeneric, terrestrial orchids, Platanthera cooperi and Platanthera praeclara, which represent widely disparate North American ecosystems. We tested whether they recruit distinct OAB communities, and whether variability in OAB communities can be linked to phenology, population size, or habitat soil. Genomic DNAs from roots of seedling, vegetative, and reproductive plants and from soil were subjected to Illumina sequencing of V4 and V5 regions of the 16S rRNA gene.
Results
We obtained 809 OAB Zero-radius Operational Taxonomic Units (ZOTUs). Despite an overlap of 209 ZOTUs that accounted for >75% relative abundances of their respective OAB communities, the overall community structures of the two orchids were distinct. Within each orchid, distinctions were detected in the OAB communities of large and small populations and the three phenological stages. The OAB ZOTUs were either absent or present with low abundances in soil associated with both orchids.
Conclusions
The two orchids exhibited preferential recruitment of known growth-promoting OAB communities from soil. Their OAB communities also showed considerable overlap despite the large environmental and geographical separation of the two host taxa. Our results lend further support to the emerging evidence that not only the fungi, but root-associated bacteria also have functional importance for orchid ecology.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | e16168 |
| Tidsskrift | American Journal of Botany |
| Vol/bind | 110 |
| Udgave nummer | 5 |
| Antal sider | 16 |
| ISSN | 0002-9122 |
| DOI | |
| Status | Udgivet - 2023 |
Bibliografisk note
Funding Information:
We acknowledge The U.S. Fish and Wildlife Service (USFWS; Grant# F12AP00638) and U.S. Office of Naval Research (U.S. Department of Defense, Cooperative Agreement No. N62473‐13‐2‐4907) for their financial support for and studies, respectively. We also thank the San Diego County Orchid Society and California Native Plant Society for providing additional research funding for . We recognize administrative and technical assistance provided by Phil Delphey (USFWS), Nancy Sather (Minnesota Department of Natural Resources), Matthew Mecklenburg and Brian Winter (The Nature Conservancy Northern Tallgrass Prairie Ecoregion), Melvin Nenneman (Valentine National Wildlife Refuge, USFWS), Bryan Stotts (Dakota Prairie Grasslands, Sheyenne Ranger District), John Pearson (Iowa Department of Natural Resources), and Christie Borkowsky (Manitoba Tall Grass Prairie Preserve) for . We also thank Nancy Sather, Jeanne Prekker (volunteer), Donna Spaeth (volunteer), Derek Anderson (MNDNR), and Dan Svedarsky (University of Minnesota, Crookston) for assisting in the field, or otherwise facilitating sampling of . We thank the Santa Catalina Island Conservancy and Cleveland National Forsest for providing research permits and for assistance in sampling . P. praeclara P. cooperi P. cooperi P. praeclara P. praeclara P. cooperi
Funding Information:
We acknowledge The U.S. Fish and Wildlife Service (USFWS; Grant# F12AP00638) and U.S. Office of Naval Research (U.S. Department of Defense, Cooperative Agreement No. N62473-13-2-4907) for their financial support for P. praeclara and P. cooperi studies, respectively. We also thank the San Diego County Orchid Society and California Native Plant Society for providing additional research funding for P. cooperi. We recognize administrative and technical assistance provided by Phil Delphey (USFWS), Nancy Sather (Minnesota Department of Natural Resources), Matthew Mecklenburg and Brian Winter (The Nature Conservancy Northern Tallgrass Prairie Ecoregion), Melvin Nenneman (Valentine National Wildlife Refuge, USFWS), Bryan Stotts (Dakota Prairie Grasslands, Sheyenne Ranger District), John Pearson (Iowa Department of Natural Resources), and Christie Borkowsky (Manitoba Tall Grass Prairie Preserve) for P. praeclara. We also thank Nancy Sather, Jeanne Prekker (volunteer), Donna Spaeth (volunteer), Derek Anderson (MNDNR), and Dan Svedarsky (University of Minnesota, Crookston) for assisting in the field, or otherwise facilitating sampling of P. praeclara. We thank the Santa Catalina Island Conservancy and Cleveland National Forsest for providing research permits and for assistance in sampling P. cooperi.
Publisher Copyright:
© 2023 Botanical Society of America.
ID: 352933091