DnaC Inactivation in Escherichia coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes

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Standard

DnaC Inactivation in Escherichia coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes. / Løbner-Olesen, Anders; Slominska-Wojewodzka, Monika; Hansen, Flemming G.; Marinus, Martin G.

I: P L o S One, Bind 3, Nr. 8, e2984, 2008.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Løbner-Olesen, A, Slominska-Wojewodzka, M, Hansen, FG & Marinus, MG 2008, 'DnaC Inactivation in Escherichia coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes', P L o S One, bind 3, nr. 8, e2984. https://doi.org/10.1371/journal.pone.0002984

APA

Løbner-Olesen, A., Slominska-Wojewodzka, M., Hansen, F. G., & Marinus, M. G. (2008). DnaC Inactivation in Escherichia coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes. P L o S One, 3(8), [e2984]. https://doi.org/10.1371/journal.pone.0002984

Vancouver

Løbner-Olesen A, Slominska-Wojewodzka M, Hansen FG, Marinus MG. DnaC Inactivation in Escherichia coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes. P L o S One. 2008;3(8). e2984. https://doi.org/10.1371/journal.pone.0002984

Author

Løbner-Olesen, Anders ; Slominska-Wojewodzka, Monika ; Hansen, Flemming G. ; Marinus, Martin G. / DnaC Inactivation in Escherichia coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes. I: P L o S One. 2008 ; Bind 3, Nr. 8.

Bibtex

@article{7c01cbb90c5242c7b7a146cd8551e308,
title = "DnaC Inactivation in Escherichia coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes",
abstract = "BACKGROUND: Initiation of chromosome replication in E. coli requires the DnaA and DnaC proteins and conditionally-lethal dnaA and dnaC mutants are often used to synchronize cell populations.METHODOLOGY/PRINCIPAL FINDINGS: DNA microarrays were used to measure mRNA steady-state levels in initiation-deficient dnaA46 and dnaC2 bacteria at permissive and non-permissive temperatures and their expression profiles were compared to MG1655 wildtype cells. For both mutants there was altered expression of genes involved in nucleotide biosynthesis at the non-permissive temperature. Transcription of the dnaA and dnaC genes was increased at the non-permissive temperature in the respective mutant strains indicating auto-regulation of both genes. Induction of the SOS regulon was observed in dnaC2 cells at 38 degrees C and 42 degrees C. Flow cytometric analysis revealed that dnaC2 mutant cells at non-permissive temperature had completed the early stages of chromosome replication initiation.CONCLUSION/SIGNIFICANCE: We suggest that in dnaC2 cells the SOS response is triggered by persistent open-complex formation at oriC and/or by arrested forks that require DnaC for replication restart.",
keywords = "Bacterial Proteins/genetics, Chromosomes, Bacterial/genetics, DNA Replication/genetics, DNA-Binding Proteins/genetics, Escherichia coli K12/genetics, Escherichia coli Proteins/antagonists & inhibitors, Flow Cytometry, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Genes, Bacterial, Genes, Lethal, Heat-Shock Proteins/genetics, Nucleotides/biosynthesis, Oligonucleotide Array Sequence Analysis, RNA, Bacterial/genetics, RNA, Messenger/genetics, SOS Response (Genetics), Thermodynamics, Transcription, Genetic",
author = "Anders L{\o}bner-Olesen and Monika Slominska-Wojewodzka and Hansen, {Flemming G.} and Marinus, {Martin G.}",
year = "2008",
doi = "10.1371/journal.pone.0002984",
language = "English",
volume = "3",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

RIS

TY - JOUR

T1 - DnaC Inactivation in Escherichia coli K-12 Induces the SOS Response and Expression of Nucleotide Biosynthesis Genes

AU - Løbner-Olesen, Anders

AU - Slominska-Wojewodzka, Monika

AU - Hansen, Flemming G.

AU - Marinus, Martin G.

PY - 2008

Y1 - 2008

N2 - BACKGROUND: Initiation of chromosome replication in E. coli requires the DnaA and DnaC proteins and conditionally-lethal dnaA and dnaC mutants are often used to synchronize cell populations.METHODOLOGY/PRINCIPAL FINDINGS: DNA microarrays were used to measure mRNA steady-state levels in initiation-deficient dnaA46 and dnaC2 bacteria at permissive and non-permissive temperatures and their expression profiles were compared to MG1655 wildtype cells. For both mutants there was altered expression of genes involved in nucleotide biosynthesis at the non-permissive temperature. Transcription of the dnaA and dnaC genes was increased at the non-permissive temperature in the respective mutant strains indicating auto-regulation of both genes. Induction of the SOS regulon was observed in dnaC2 cells at 38 degrees C and 42 degrees C. Flow cytometric analysis revealed that dnaC2 mutant cells at non-permissive temperature had completed the early stages of chromosome replication initiation.CONCLUSION/SIGNIFICANCE: We suggest that in dnaC2 cells the SOS response is triggered by persistent open-complex formation at oriC and/or by arrested forks that require DnaC for replication restart.

AB - BACKGROUND: Initiation of chromosome replication in E. coli requires the DnaA and DnaC proteins and conditionally-lethal dnaA and dnaC mutants are often used to synchronize cell populations.METHODOLOGY/PRINCIPAL FINDINGS: DNA microarrays were used to measure mRNA steady-state levels in initiation-deficient dnaA46 and dnaC2 bacteria at permissive and non-permissive temperatures and their expression profiles were compared to MG1655 wildtype cells. For both mutants there was altered expression of genes involved in nucleotide biosynthesis at the non-permissive temperature. Transcription of the dnaA and dnaC genes was increased at the non-permissive temperature in the respective mutant strains indicating auto-regulation of both genes. Induction of the SOS regulon was observed in dnaC2 cells at 38 degrees C and 42 degrees C. Flow cytometric analysis revealed that dnaC2 mutant cells at non-permissive temperature had completed the early stages of chromosome replication initiation.CONCLUSION/SIGNIFICANCE: We suggest that in dnaC2 cells the SOS response is triggered by persistent open-complex formation at oriC and/or by arrested forks that require DnaC for replication restart.

KW - Bacterial Proteins/genetics

KW - Chromosomes, Bacterial/genetics

KW - DNA Replication/genetics

KW - DNA-Binding Proteins/genetics

KW - Escherichia coli K12/genetics

KW - Escherichia coli Proteins/antagonists & inhibitors

KW - Flow Cytometry

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Bacterial

KW - Genes, Bacterial

KW - Genes, Lethal

KW - Heat-Shock Proteins/genetics

KW - Nucleotides/biosynthesis

KW - Oligonucleotide Array Sequence Analysis

KW - RNA, Bacterial/genetics

KW - RNA, Messenger/genetics

KW - SOS Response (Genetics)

KW - Thermodynamics

KW - Transcription, Genetic

U2 - 10.1371/journal.pone.0002984

DO - 10.1371/journal.pone.0002984

M3 - Journal article

C2 - 18714349

VL - 3

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 8

M1 - e2984

ER -

ID: 200971647