Dysfunctional MreB inhibits chromosome segregation in Escherichia coli

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Standard

Dysfunctional MreB inhibits chromosome segregation in Escherichia coli. / Kruse, Thomas; Møller-Jensen, Jakob; Løbner-Olesen, Anders; Gerdes, Kenn.

I: E M B O Journal, Bind 22, Nr. 19, 2003, s. 5283-5292.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kruse, T, Møller-Jensen, J, Løbner-Olesen, A & Gerdes, K 2003, 'Dysfunctional MreB inhibits chromosome segregation in Escherichia coli', E M B O Journal, bind 22, nr. 19, s. 5283-5292. https://doi.org/10.1093/emboj/cdg504

APA

Kruse, T., Møller-Jensen, J., Løbner-Olesen, A., & Gerdes, K. (2003). Dysfunctional MreB inhibits chromosome segregation in Escherichia coli. E M B O Journal, 22(19), 5283-5292. https://doi.org/10.1093/emboj/cdg504

Vancouver

Kruse T, Møller-Jensen J, Løbner-Olesen A, Gerdes K. Dysfunctional MreB inhibits chromosome segregation in Escherichia coli. E M B O Journal. 2003;22(19):5283-5292. https://doi.org/10.1093/emboj/cdg504

Author

Kruse, Thomas ; Møller-Jensen, Jakob ; Løbner-Olesen, Anders ; Gerdes, Kenn. / Dysfunctional MreB inhibits chromosome segregation in Escherichia coli. I: E M B O Journal. 2003 ; Bind 22, Nr. 19. s. 5283-5292.

Bibtex

@article{d2720c675b184244a6f121ed9a73ce40,
title = "Dysfunctional MreB inhibits chromosome segregation in Escherichia coli",
abstract = "The mechanism of prokaryotic chromosome segregation is not known. MreB, an actin homolog, is a shape-determining factor in rod-shaped prokaryotic cells. Using immunofluorescence microscopy we found that MreB of Escherichia coli formed helical filaments located beneath the cell surface. Flow cytometric and cytological analyses indicated that MreB-depleted cells segregated their chromosomes in pairs, consistent with chromosome cohesion. Overexpression of wild-type MreB inhibited cell division but did not perturb chromosome segregation. Overexpression of mutant forms of MreB inhibited cell division, caused abnormal MreB filament morphology and induced severe localization defects of the nucleoid and of the oriC and terC chromosomal regions. The chromosomal terminus regions appeared cohered in both MreB-depleted cells and in cells overexpressing mutant forms of MreB. Our observations indicate that MreB filaments participate in directional chromosome movement and segregation.",
keywords = "Amino Acid Sequence, Chromosome Segregation/physiology, DNA-Binding Proteins/metabolism, Escherichia coli/metabolism, Escherichia coli Proteins/genetics, Molecular Sequence Data, Mutation, Origin Recognition Complex, RNA/metabolism, Telomerase/metabolism, Viral Proteins/metabolism",
author = "Thomas Kruse and Jakob M{\o}ller-Jensen and Anders L{\o}bner-Olesen and Kenn Gerdes",
year = "2003",
doi = "10.1093/emboj/cdg504",
language = "English",
volume = "22",
pages = "5283--5292",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell",
number = "19",

}

RIS

TY - JOUR

T1 - Dysfunctional MreB inhibits chromosome segregation in Escherichia coli

AU - Kruse, Thomas

AU - Møller-Jensen, Jakob

AU - Løbner-Olesen, Anders

AU - Gerdes, Kenn

PY - 2003

Y1 - 2003

N2 - The mechanism of prokaryotic chromosome segregation is not known. MreB, an actin homolog, is a shape-determining factor in rod-shaped prokaryotic cells. Using immunofluorescence microscopy we found that MreB of Escherichia coli formed helical filaments located beneath the cell surface. Flow cytometric and cytological analyses indicated that MreB-depleted cells segregated their chromosomes in pairs, consistent with chromosome cohesion. Overexpression of wild-type MreB inhibited cell division but did not perturb chromosome segregation. Overexpression of mutant forms of MreB inhibited cell division, caused abnormal MreB filament morphology and induced severe localization defects of the nucleoid and of the oriC and terC chromosomal regions. The chromosomal terminus regions appeared cohered in both MreB-depleted cells and in cells overexpressing mutant forms of MreB. Our observations indicate that MreB filaments participate in directional chromosome movement and segregation.

AB - The mechanism of prokaryotic chromosome segregation is not known. MreB, an actin homolog, is a shape-determining factor in rod-shaped prokaryotic cells. Using immunofluorescence microscopy we found that MreB of Escherichia coli formed helical filaments located beneath the cell surface. Flow cytometric and cytological analyses indicated that MreB-depleted cells segregated their chromosomes in pairs, consistent with chromosome cohesion. Overexpression of wild-type MreB inhibited cell division but did not perturb chromosome segregation. Overexpression of mutant forms of MreB inhibited cell division, caused abnormal MreB filament morphology and induced severe localization defects of the nucleoid and of the oriC and terC chromosomal regions. The chromosomal terminus regions appeared cohered in both MreB-depleted cells and in cells overexpressing mutant forms of MreB. Our observations indicate that MreB filaments participate in directional chromosome movement and segregation.

KW - Amino Acid Sequence

KW - Chromosome Segregation/physiology

KW - DNA-Binding Proteins/metabolism

KW - Escherichia coli/metabolism

KW - Escherichia coli Proteins/genetics

KW - Molecular Sequence Data

KW - Mutation

KW - Origin Recognition Complex

KW - RNA/metabolism

KW - Telomerase/metabolism

KW - Viral Proteins/metabolism

U2 - 10.1093/emboj/cdg504

DO - 10.1093/emboj/cdg504

M3 - Journal article

C2 - 14517265

VL - 22

SP - 5283

EP - 5292

JO - E M B O Journal

JF - E M B O Journal

SN - 0261-4189

IS - 19

ER -

ID: 200972073