Energy Starvation Induces a Cell Cycle Arrest in Escherichia coli by Triggering Degradation of the DnaA Initiator Protein

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Standard

Energy Starvation Induces a Cell Cycle Arrest in Escherichia coli by Triggering Degradation of the DnaA Initiator Protein. / Charbon, Godefroid; Mendoza-Chamizo, Belén; Campion, Christopher; Li, Xiaobo; Jensen, Peter Ruhdal; Frimodt-Møller, Jakob; Løbner-Olesen, Anders.

I: Frontiers in Molecular Biosciences, Bind 8, 629953, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Charbon, G, Mendoza-Chamizo, B, Campion, C, Li, X, Jensen, PR, Frimodt-Møller, J & Løbner-Olesen, A 2021, 'Energy Starvation Induces a Cell Cycle Arrest in Escherichia coli by Triggering Degradation of the DnaA Initiator Protein', Frontiers in Molecular Biosciences, bind 8, 629953. https://doi.org/10.3389/fmolb.2021.629953

APA

Charbon, G., Mendoza-Chamizo, B., Campion, C., Li, X., Jensen, P. R., Frimodt-Møller, J., & Løbner-Olesen, A. (2021). Energy Starvation Induces a Cell Cycle Arrest in Escherichia coli by Triggering Degradation of the DnaA Initiator Protein. Frontiers in Molecular Biosciences, 8, [629953]. https://doi.org/10.3389/fmolb.2021.629953

Vancouver

Charbon G, Mendoza-Chamizo B, Campion C, Li X, Jensen PR, Frimodt-Møller J o.a. Energy Starvation Induces a Cell Cycle Arrest in Escherichia coli by Triggering Degradation of the DnaA Initiator Protein. Frontiers in Molecular Biosciences. 2021;8. 629953. https://doi.org/10.3389/fmolb.2021.629953

Author

Charbon, Godefroid ; Mendoza-Chamizo, Belén ; Campion, Christopher ; Li, Xiaobo ; Jensen, Peter Ruhdal ; Frimodt-Møller, Jakob ; Løbner-Olesen, Anders. / Energy Starvation Induces a Cell Cycle Arrest in Escherichia coli by Triggering Degradation of the DnaA Initiator Protein. I: Frontiers in Molecular Biosciences. 2021 ; Bind 8.

Bibtex

@article{208d561c6ca74a57b723eea3fb0dcbb5,

title = "Energy Starvation Induces a Cell Cycle Arrest in Escherichia coli by Triggering Degradation of the DnaA Initiator Protein",

abstract = "During steady-state Escherichia coli growth, the amount and activity of the initiator protein, DnaA, controls chromosome replication tightly so that initiation only takes place once per origin in each cell cycle, regardless of growth conditions. However, little is known about the mechanisms involved during transitions from one environmental condition to another or during starvation stress. ATP depletion is one of the consequences of long-term carbon starvation. Here we show that DnaA is degraded in ATP-depleted cells. A chromosome replication initiation block is apparent in such cells as no new rounds of DNA replication are initiated while replication events that have already started proceed to completion.",

author = "Godefroid Charbon and Bel{\'e}n Mendoza-Chamizo and Christopher Campion and Xiaobo Li and Jensen, {Peter Ruhdal} and Jakob Frimodt-M{\o}ller and Anders L{\o}bner-Olesen",

note = "Copyright {\textcopyright} 2021 Charbon, Mendoza-Chamizo, Campion, Li, Jensen, Frimodt-M{\o}ller and L{\o}bner-Olesen.",

year = "2021",

doi = "10.3389/fmolb.2021.629953",

language = "English",

volume = "8",

journal = "Frontiers in Molecular Biosciences",

issn = "2296-889X",

publisher = "Frontiers Media",

}

RIS

TY - JOUR

T1 - Energy Starvation Induces a Cell Cycle Arrest in Escherichia coli by Triggering Degradation of the DnaA Initiator Protein

AU - Charbon, Godefroid

AU - Mendoza-Chamizo, Belén

AU - Campion, Christopher

AU - Li, Xiaobo

AU - Jensen, Peter Ruhdal

AU - Frimodt-Møller, Jakob

AU - Løbner-Olesen, Anders

PY - 2021

Y1 - 2021

N2 - During steady-state Escherichia coli growth, the amount and activity of the initiator protein, DnaA, controls chromosome replication tightly so that initiation only takes place once per origin in each cell cycle, regardless of growth conditions. However, little is known about the mechanisms involved during transitions from one environmental condition to another or during starvation stress. ATP depletion is one of the consequences of long-term carbon starvation. Here we show that DnaA is degraded in ATP-depleted cells. A chromosome replication initiation block is apparent in such cells as no new rounds of DNA replication are initiated while replication events that have already started proceed to completion.

AB - During steady-state Escherichia coli growth, the amount and activity of the initiator protein, DnaA, controls chromosome replication tightly so that initiation only takes place once per origin in each cell cycle, regardless of growth conditions. However, little is known about the mechanisms involved during transitions from one environmental condition to another or during starvation stress. ATP depletion is one of the consequences of long-term carbon starvation. Here we show that DnaA is degraded in ATP-depleted cells. A chromosome replication initiation block is apparent in such cells as no new rounds of DNA replication are initiated while replication events that have already started proceed to completion.

U2 - 10.3389/fmolb.2021.629953

DO - 10.3389/fmolb.2021.629953

M3 - Journal article

C2 - 34055872

VL - 8

JO - Frontiers in Molecular Biosciences

JF - Frontiers in Molecular Biosciences

SN - 2296-889X

M1 - 629953

ER -

ID: 270559360

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