Expression of the Escherichia coli dam gene

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Standard

Expression of the Escherichia coli dam gene. / Løbner-Olesen, A; Boye, E; Marinus, M G.

I: Molecular Microbiology, Bind 6, Nr. 13, 07.1992, s. 1841-51.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Løbner-Olesen, A, Boye, E & Marinus, MG 1992, 'Expression of the Escherichia coli dam gene', Molecular Microbiology, bind 6, nr. 13, s. 1841-51.

APA

Løbner-Olesen, A., Boye, E., & Marinus, M. G. (1992). Expression of the Escherichia coli dam gene. Molecular Microbiology, 6(13), 1841-51.

Vancouver

Løbner-Olesen A, Boye E, Marinus MG. Expression of the Escherichia coli dam gene. Molecular Microbiology. 1992 jul.;6(13):1841-51.

Author

Løbner-Olesen, A ; Boye, E ; Marinus, M G. / Expression of the Escherichia coli dam gene. I: Molecular Microbiology. 1992 ; Bind 6, Nr. 13. s. 1841-51.

Bibtex

@article{293bbffbd8d04b918a7e3c4325b9f4e8,
title = "Expression of the Escherichia coli dam gene",
abstract = "The Escherichia coli dam gene and upstream sequences were cloned from the Kohara phage 4D4. Five promoters were found to contribute to dam gene transcription. P1 and P2 (the major promoter) were situated approximately 3.5 kb upstream of the structural gene, P3 was within the aroB gene, P4 was within the urf74.3 gene, and P5 was in the urf74.3-dam intergenic region. The nucleotide sequence of 2280 bp of DNA containing P1 and P2 was determined and shown to have the potential to encode a protein of approximately 16 kDa between P1, P2 and the aroB gene. This 16 kDa open reading frame has been identified as aroK, the gene for shikimic acid kinase I. Thus the dam gene is part of an operon containing aroK, aroB, urf74.3, and dam. The transcriptional start points of the promoters were determined. A comparison of their nucleotide sequences suggested that P1-P4 were all recognized by the sigma 70 subunit of the RNA polymerase.",
keywords = "Amino Acid Sequence, Base Sequence, Cloning, Molecular, Escherichia coli/enzymology, Escherichia coli Proteins, Flow Cytometry, Gene Expression Regulation, Bacterial, Genes, Bacterial, Methyltransferases/genetics, Molecular Sequence Data, Mutation, Operon, Promoter Regions, Genetic, Site-Specific DNA-Methyltransferase (Adenine-Specific), Transcription Factors/genetics",
author = "A L{\o}bner-Olesen and E Boye and Marinus, {M G}",
year = "1992",
month = jul,
language = "English",
volume = "6",
pages = "1841--51",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "13",

}

RIS

TY - JOUR

T1 - Expression of the Escherichia coli dam gene

AU - Løbner-Olesen, A

AU - Boye, E

AU - Marinus, M G

PY - 1992/7

Y1 - 1992/7

N2 - The Escherichia coli dam gene and upstream sequences were cloned from the Kohara phage 4D4. Five promoters were found to contribute to dam gene transcription. P1 and P2 (the major promoter) were situated approximately 3.5 kb upstream of the structural gene, P3 was within the aroB gene, P4 was within the urf74.3 gene, and P5 was in the urf74.3-dam intergenic region. The nucleotide sequence of 2280 bp of DNA containing P1 and P2 was determined and shown to have the potential to encode a protein of approximately 16 kDa between P1, P2 and the aroB gene. This 16 kDa open reading frame has been identified as aroK, the gene for shikimic acid kinase I. Thus the dam gene is part of an operon containing aroK, aroB, urf74.3, and dam. The transcriptional start points of the promoters were determined. A comparison of their nucleotide sequences suggested that P1-P4 were all recognized by the sigma 70 subunit of the RNA polymerase.

AB - The Escherichia coli dam gene and upstream sequences were cloned from the Kohara phage 4D4. Five promoters were found to contribute to dam gene transcription. P1 and P2 (the major promoter) were situated approximately 3.5 kb upstream of the structural gene, P3 was within the aroB gene, P4 was within the urf74.3 gene, and P5 was in the urf74.3-dam intergenic region. The nucleotide sequence of 2280 bp of DNA containing P1 and P2 was determined and shown to have the potential to encode a protein of approximately 16 kDa between P1, P2 and the aroB gene. This 16 kDa open reading frame has been identified as aroK, the gene for shikimic acid kinase I. Thus the dam gene is part of an operon containing aroK, aroB, urf74.3, and dam. The transcriptional start points of the promoters were determined. A comparison of their nucleotide sequences suggested that P1-P4 were all recognized by the sigma 70 subunit of the RNA polymerase.

KW - Amino Acid Sequence

KW - Base Sequence

KW - Cloning, Molecular

KW - Escherichia coli/enzymology

KW - Escherichia coli Proteins

KW - Flow Cytometry

KW - Gene Expression Regulation, Bacterial

KW - Genes, Bacterial

KW - Methyltransferases/genetics

KW - Molecular Sequence Data

KW - Mutation

KW - Operon

KW - Promoter Regions, Genetic

KW - Site-Specific DNA-Methyltransferase (Adenine-Specific)

KW - Transcription Factors/genetics

M3 - Journal article

C2 - 1630320

VL - 6

SP - 1841

EP - 1851

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 13

ER -

ID: 200972937