Gut microbiota signatures in inflammatory bowel disease
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Gut microbiota signatures in inflammatory bowel disease. / Vestergaard, Marie Vibeke; Allin, Kristine H.; Eriksen, Carsten; Zakerska-Banaszak, Oliwia; Arasaradnam, Ramesh P.; Alam, Mohammad T.; Kristiansen, Karsten; Brix, Susanne; Jess, Tine.
I: United European Gastroenterology Journal, Bind 12, Nr. 1, 2024, s. 22-33.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Gut microbiota signatures in inflammatory bowel disease
AU - Vestergaard, Marie Vibeke
AU - Allin, Kristine H.
AU - Eriksen, Carsten
AU - Zakerska-Banaszak, Oliwia
AU - Arasaradnam, Ramesh P.
AU - Alam, Mohammad T.
AU - Kristiansen, Karsten
AU - Brix, Susanne
AU - Jess, Tine
N1 - Publisher Copyright: © 2023 The Authors. United European Gastroenterology Journal published by Wiley Periodicals LLC on behalf of United European Gastroenterology.
PY - 2024
Y1 - 2024
N2 - Background: Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), affect millions of people worldwide with increasing incidence. Objectives: Several studies have shown a link between gut microbiota composition and IBD, but results are often limited by small sample sizes. We aimed to re-analyze publicly available fecal microbiota data from IBD patients. Methods: We extracted original fecal 16S rRNA amplicon sequencing data from 45 cohorts of IBD patients and healthy individuals using the BioProject database at the National Center for Biotechnology Information. Unlike previous meta-analyses, we merged all study cohorts into a single dataset, including sex, age, geography, and disease information, based on which microbiota signatures were analyzed, while accounting for varying technical platforms. Results: Among 2518 individuals in the combined dataset, we discovered a hitherto unseen number of genera associated with IBD. A total of 77 genera associated with CD, of which 38 were novel associations, and a total of 64 genera associated with UC, of which 28 represented novel associations. Signatures were robust across different technical platforms and geographic locations. Reduced alpha diversity in IBD compared to healthy individuals, in CD compared to UC, and altered microbiota composition (beta diversity) in UC and especially in CD as compared to healthy individuals were found. Conclusions: Combining original microbiota data from 45 cohorts, we identified a hitherto unseen large number of genera associated with IBD. Identification of microbiota features robustly associated with CD and UC may pave the way for the identification of new treatment targets.
AB - Background: Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), affect millions of people worldwide with increasing incidence. Objectives: Several studies have shown a link between gut microbiota composition and IBD, but results are often limited by small sample sizes. We aimed to re-analyze publicly available fecal microbiota data from IBD patients. Methods: We extracted original fecal 16S rRNA amplicon sequencing data from 45 cohorts of IBD patients and healthy individuals using the BioProject database at the National Center for Biotechnology Information. Unlike previous meta-analyses, we merged all study cohorts into a single dataset, including sex, age, geography, and disease information, based on which microbiota signatures were analyzed, while accounting for varying technical platforms. Results: Among 2518 individuals in the combined dataset, we discovered a hitherto unseen number of genera associated with IBD. A total of 77 genera associated with CD, of which 38 were novel associations, and a total of 64 genera associated with UC, of which 28 represented novel associations. Signatures were robust across different technical platforms and geographic locations. Reduced alpha diversity in IBD compared to healthy individuals, in CD compared to UC, and altered microbiota composition (beta diversity) in UC and especially in CD as compared to healthy individuals were found. Conclusions: Combining original microbiota data from 45 cohorts, we identified a hitherto unseen large number of genera associated with IBD. Identification of microbiota features robustly associated with CD and UC may pave the way for the identification of new treatment targets.
KW - 16S rRNA amplicon sequencing
KW - Crohn's disease
KW - Faecalibacterium
KW - IBD
KW - Lachnospiraceae NK4A136 group
KW - microbiome
KW - microbiota
KW - Roseburia
KW - Turicibacter
KW - ulcerative colitis
U2 - 10.1002/ueg2.12485
DO - 10.1002/ueg2.12485
M3 - Journal article
C2 - 38041519
AN - SCOPUS:85178425963
VL - 12
SP - 22
EP - 33
JO - United European Gastroenterology Journal
JF - United European Gastroenterology Journal
SN - 2050-6406
IS - 1
ER -
ID: 375724018