In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila
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NanoDam is a technique for genome-wide profiling of the binding targets of any endogenously tagged chromatin-binding protein in vivo, without the need for antibodies, crosslinking, or immunoprecipitation. Here, we explain the procedure for NanoDam experiments in Drosophila, starting from a genetic cross, to the generation of sequencing libraries and, finally, bioinformatic analysis. This protocol can be readily adapted for use in other model systems after simple modifications.
Originalsprog | Engelsk |
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Artikelnummer | 101788 |
Tidsskrift | STAR Protocols |
Vol/bind | 3 |
Udgave nummer | 4 |
Antal sider | 30 |
ISSN | 2666-1667 |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
This work was funded by Wellcome Trust PhD Studentships to A.E.H. ( 102454 ) and J.L.Y.T. ( 203798 ), an EMBO Long Term Fellowship to P.M.F. and Wellcome Trust Programme Grant ( 092545 ), Wellcome Trust Senior Investigator Award ( 103792/Z/14/Z ) and Royal Society Darwin Trust Research Professorship ( RP150061 ) to A.H.B. A.H.B. acknowledges core funding to the Gurdon Institute from the Wellcome Trust ( 092096 ) and CRUK ( C6946/A14492 ).
Publisher Copyright:
© 2022 The Authors
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