Isolation of cancer stem cells from squamous cell carcinoma

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Standard

Isolation of cancer stem cells from squamous cell carcinoma. / Fontenete, Silvia; Perez-Moreno, Mirna.

Skin Stem Cells: Methods and Protocols. red. / Kursad Turksen. 2. ed. udg. Humana Press, 2019. s. 407-414 (Methods in Molecular Biology, Bind 1879).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Fontenete, S & Perez-Moreno, M 2019, Isolation of cancer stem cells from squamous cell carcinoma. i K Turksen (red.), Skin Stem Cells: Methods and Protocols. 2. ed. udg, Humana Press, Methods in Molecular Biology, bind 1879, s. 407-414. https://doi.org/10.1007/7651_2018_162

APA

Fontenete, S., & Perez-Moreno, M. (2019). Isolation of cancer stem cells from squamous cell carcinoma. I K. Turksen (red.), Skin Stem Cells: Methods and Protocols (2. ed. udg., s. 407-414). Humana Press. Methods in Molecular Biology Bind 1879 https://doi.org/10.1007/7651_2018_162

Vancouver

Fontenete S, Perez-Moreno M. Isolation of cancer stem cells from squamous cell carcinoma. I Turksen K, red., Skin Stem Cells: Methods and Protocols. 2. ed. udg. Humana Press. 2019. s. 407-414. (Methods in Molecular Biology, Bind 1879). https://doi.org/10.1007/7651_2018_162

Author

Fontenete, Silvia ; Perez-Moreno, Mirna. / Isolation of cancer stem cells from squamous cell carcinoma. Skin Stem Cells: Methods and Protocols. red. / Kursad Turksen. 2. ed. udg. Humana Press, 2019. s. 407-414 (Methods in Molecular Biology, Bind 1879).

Bibtex

@inbook{f7a48bb806034e76b7fd4374efc08222,
title = "Isolation of cancer stem cells from squamous cell carcinoma",
abstract = "Different cancer stem cell (CSC) populations can be found in many types of cancer, including squamous cell carcinoma (SSC). Diverse reports showed that CSC play a crucial role in the relapse of different types of cancer. CSC sustains tumor growth due to their capacity to self-renew and their potential to initiate secondary tumors with metastatic cancer features. Therefore, the development of methods for the isolation of CSC is a key step to explore the mechanisms underlying CSC maintenance. In this chapter, we provide a method for isolating CSC from cutaneous SSC using immunofluorescence labeling to allow the specific purification of CSC by fluorescence-activated cell sorting (FACS). This method is based on the use of CSC membrane markers, allowing as well the isolation CSC from different mouse strains.",
keywords = "Cancer stem cells, FACS, Mouse models, Skin, Squamous cell carcinoma",
author = "Silvia Fontenete and Mirna Perez-Moreno",
year = "2019",
doi = "10.1007/7651_2018_162",
language = "English",
isbn = "978-1-4939-8869-3",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "407--414",
editor = "Kursad Turksen",
booktitle = "Skin Stem Cells",
address = "United States",
edition = "2. ed.",

}

RIS

TY - CHAP

T1 - Isolation of cancer stem cells from squamous cell carcinoma

AU - Fontenete, Silvia

AU - Perez-Moreno, Mirna

PY - 2019

Y1 - 2019

N2 - Different cancer stem cell (CSC) populations can be found in many types of cancer, including squamous cell carcinoma (SSC). Diverse reports showed that CSC play a crucial role in the relapse of different types of cancer. CSC sustains tumor growth due to their capacity to self-renew and their potential to initiate secondary tumors with metastatic cancer features. Therefore, the development of methods for the isolation of CSC is a key step to explore the mechanisms underlying CSC maintenance. In this chapter, we provide a method for isolating CSC from cutaneous SSC using immunofluorescence labeling to allow the specific purification of CSC by fluorescence-activated cell sorting (FACS). This method is based on the use of CSC membrane markers, allowing as well the isolation CSC from different mouse strains.

AB - Different cancer stem cell (CSC) populations can be found in many types of cancer, including squamous cell carcinoma (SSC). Diverse reports showed that CSC play a crucial role in the relapse of different types of cancer. CSC sustains tumor growth due to their capacity to self-renew and their potential to initiate secondary tumors with metastatic cancer features. Therefore, the development of methods for the isolation of CSC is a key step to explore the mechanisms underlying CSC maintenance. In this chapter, we provide a method for isolating CSC from cutaneous SSC using immunofluorescence labeling to allow the specific purification of CSC by fluorescence-activated cell sorting (FACS). This method is based on the use of CSC membrane markers, allowing as well the isolation CSC from different mouse strains.

KW - Cancer stem cells

KW - FACS

KW - Mouse models

KW - Skin

KW - Squamous cell carcinoma

U2 - 10.1007/7651_2018_162

DO - 10.1007/7651_2018_162

M3 - Book chapter

C2 - 29790094

AN - SCOPUS:85053443336

SN - 978-1-4939-8869-3

T3 - Methods in Molecular Biology

SP - 407

EP - 414

BT - Skin Stem Cells

A2 - Turksen, Kursad

PB - Humana Press

ER -

ID: 215920873