Legionella maioricensis sp. nov., a new species isolated from the hot water distribution systems of a hospital and a shopping center during routine sampling
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Legionella maioricensis sp. nov., a new species isolated from the hot water distribution systems of a hospital and a shopping center during routine sampling. / Crespi, Sebastian; Drašar, Vladimír; Salvà-Serra, Francisco; Jaén-Luchoro, Daniel; Piñeiro-Iglesias, Beatriz; Lindemann, Paul Christoffer; Aliaga-Lozano, Francisco; Fernández-Juárez, Victor; Coll-García, Guillem; Moore, Edward R.B.; Bennasar-Figueras, Antoni.
I: International Journal of Systematic and Evolutionary Microbiology, Bind 73, Nr. 1, 005686, 2023.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Legionella maioricensis sp. nov., a new species isolated from the hot water distribution systems of a hospital and a shopping center during routine sampling
AU - Crespi, Sebastian
AU - Drašar, Vladimír
AU - Salvà-Serra, Francisco
AU - Jaén-Luchoro, Daniel
AU - Piñeiro-Iglesias, Beatriz
AU - Lindemann, Paul Christoffer
AU - Aliaga-Lozano, Francisco
AU - Fernández-Juárez, Victor
AU - Coll-García, Guillem
AU - Moore, Edward R.B.
AU - Bennasar-Figueras, Antoni
N1 - Publisher Copyright: © 2023 The Authors.
PY - 2023
Y1 - 2023
N2 - Two Legionella-like strains isolated from hot water distribution systems in 2012 have been characterized phenotypically, bio-chemically and genomically in terms of DNA relatedness. Both strains, HCPI-6T and EUR-108, exhibited biochemical phenotypic profiles typical of Legionella species. Cells were Gram-negative motile rods which grew on BCYEα agar but not on blood agar and displayed phenotypic characteristics typical of the family Legionellaceae, including a requirement for l-cysteine and testing catalase positive. Both strains were negative for oxidase, urease, nitrate reduction and hippurate negative, and non-fermentative. The major ubiquinone was Q12 (59.4 % HCPI-6T) and the dominant fatty acids were C16: 1 ω7c (28.4 % HCPI-6T, ≈16 % EUR-108), C16: 0 iso (≈22.5 % and ≈13 %) and C15: 0 anteiso (19.5 % and ≈23.5 %, respectively). The percent G+C content of genomic DNA was determined to be 39.3 mol %. The 16S rRNA gene, mip sequence and comparative genome sequence-based analyses (average nucleotide identity, ANI; digital DNA–DNA hybridization, dDDH; and phylogenomic treeing) demonstrated that the strains represent a new species of the genus Legionella. The analysis based on the 16S rRNA gene sequences showed that the sequence similarities for both strains ranged from 98.8–90.1 % to other members of the genus. The core genome-based phylogenomic tree (protein-concatemer tree based on concatenation of 418 proteins present in single copy) revealed that these two strains clearly form a separate cluster within the genus Legionella. ANI and dDDH values confirmed the distinctiveness of the strains. Based on the genomic, genotypic and phenotypic findings from a polyphasic study, the isolates are considered to represent a single novel species, for which the name Legionella maioricensis sp. nov. is proposed. The type strain is HCPI-6T (=CCUG 75071T=CECT 30569T).
AB - Two Legionella-like strains isolated from hot water distribution systems in 2012 have been characterized phenotypically, bio-chemically and genomically in terms of DNA relatedness. Both strains, HCPI-6T and EUR-108, exhibited biochemical phenotypic profiles typical of Legionella species. Cells were Gram-negative motile rods which grew on BCYEα agar but not on blood agar and displayed phenotypic characteristics typical of the family Legionellaceae, including a requirement for l-cysteine and testing catalase positive. Both strains were negative for oxidase, urease, nitrate reduction and hippurate negative, and non-fermentative. The major ubiquinone was Q12 (59.4 % HCPI-6T) and the dominant fatty acids were C16: 1 ω7c (28.4 % HCPI-6T, ≈16 % EUR-108), C16: 0 iso (≈22.5 % and ≈13 %) and C15: 0 anteiso (19.5 % and ≈23.5 %, respectively). The percent G+C content of genomic DNA was determined to be 39.3 mol %. The 16S rRNA gene, mip sequence and comparative genome sequence-based analyses (average nucleotide identity, ANI; digital DNA–DNA hybridization, dDDH; and phylogenomic treeing) demonstrated that the strains represent a new species of the genus Legionella. The analysis based on the 16S rRNA gene sequences showed that the sequence similarities for both strains ranged from 98.8–90.1 % to other members of the genus. The core genome-based phylogenomic tree (protein-concatemer tree based on concatenation of 418 proteins present in single copy) revealed that these two strains clearly form a separate cluster within the genus Legionella. ANI and dDDH values confirmed the distinctiveness of the strains. Based on the genomic, genotypic and phenotypic findings from a polyphasic study, the isolates are considered to represent a single novel species, for which the name Legionella maioricensis sp. nov. is proposed. The type strain is HCPI-6T (=CCUG 75071T=CECT 30569T).
KW - 16S rRNA gene
KW - average nucleotide identity
KW - digital DNA–DNA hybridization
KW - genome comparison
KW - hot water
KW - Legionella maioricensis
KW - mip
KW - novel species
KW - phylogenomics
KW - polyphasic taxonomy
U2 - 10.1099/ijsem.0.005686
DO - 10.1099/ijsem.0.005686
M3 - Journal article
C2 - 36748493
AN - SCOPUS:85146325443
VL - 73
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 1
M1 - 005686
ER -
ID: 335055002