Long-term conservation tillage with reduced nitrogen fertilization intensity can improve winter wheat health via positive plant–microorganism feedback in the rhizosphere
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Long-term conservation tillage with reduced nitrogen fertilization intensity can improve winter wheat health via positive plant–microorganism feedback in the rhizosphere. / Behr, Jan Helge; Kuhl-Nagel, Theresa; Sommermann, Loreen; Moradtalab, Narges; Chowdhury, Soumitra Paul; Schloter, Michael; Windisch, Saskia; Schellenberg, Ingo; Maccario, Lorrie; Sørensen, Søren J.; Rothballer, Michael; Geistlinger, Joerg; Smalla, Kornelia; Ludewig, Uwe; Neumann, Günter; Grosch, Rita; Babin, Doreen.
I: FEMS Microbiology Ecology, Bind 100, Nr. 2, fiae003, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Long-term conservation tillage with reduced nitrogen fertilization intensity can improve winter wheat health via positive plant–microorganism feedback in the rhizosphere
AU - Behr, Jan Helge
AU - Kuhl-Nagel, Theresa
AU - Sommermann, Loreen
AU - Moradtalab, Narges
AU - Chowdhury, Soumitra Paul
AU - Schloter, Michael
AU - Windisch, Saskia
AU - Schellenberg, Ingo
AU - Maccario, Lorrie
AU - Sørensen, Søren J.
AU - Rothballer, Michael
AU - Geistlinger, Joerg
AU - Smalla, Kornelia
AU - Ludewig, Uwe
AU - Neumann, Günter
AU - Grosch, Rita
AU - Babin, Doreen
N1 - Publisher Copyright: © 2024 Oxford University Press. All rights reserved.
PY - 2024
Y1 - 2024
N2 - Microbiome-based solutions are regarded key for sustainable agroecosystems. However, it is unclear how agricultural practices affect the rhizosphere microbiome, plant–microorganism interactions and crop performance under field conditions. Therefore, we installed root observation windows in a winter wheat field cultivated either under long-term mouldboard plough (MP) or cultivator tillage (CT). Each tillage practice was also compared at two nitrogen (N) fertilization intensities, intensive (recommended N-supply with pesticides/growth regulators) or extensive (reduced N-supply, no fungicides/growth regulators). Shoot biomass, root exudates and rhizosphere metabolites, physiological stress indicators, and gene expression were analyzed together with the rhizosphere microbiome (bacterial/archaeal 16S rRNA gene, fungal ITS amplicon, and shotgun metagenome sequencing) shortly before flowering. Compared to MP, the rhizosphere of CT winter wheat contained more primary and secondary metabolites, especially benzoxazinoid derivatives. Potential copiotrophic and plant-beneficial taxa (e.g. Bacillus, Devosia, and Trichoderma) as well as functional genes (e.g. siderophore production, trehalose synthase, and ACC deaminase) were enriched in the CT rhizosphere, suggesting that tillage affected belowground plant–microorganism interactions. In addition, physiological stress markers were suppressed in CT winter wheat compared to MP. In summary, tillage practice was a major driver of crop performance, root deposits, and rhizosphere microbiome interactions, while the N-fertilization intensity was also relevant, but less important.
AB - Microbiome-based solutions are regarded key for sustainable agroecosystems. However, it is unclear how agricultural practices affect the rhizosphere microbiome, plant–microorganism interactions and crop performance under field conditions. Therefore, we installed root observation windows in a winter wheat field cultivated either under long-term mouldboard plough (MP) or cultivator tillage (CT). Each tillage practice was also compared at two nitrogen (N) fertilization intensities, intensive (recommended N-supply with pesticides/growth regulators) or extensive (reduced N-supply, no fungicides/growth regulators). Shoot biomass, root exudates and rhizosphere metabolites, physiological stress indicators, and gene expression were analyzed together with the rhizosphere microbiome (bacterial/archaeal 16S rRNA gene, fungal ITS amplicon, and shotgun metagenome sequencing) shortly before flowering. Compared to MP, the rhizosphere of CT winter wheat contained more primary and secondary metabolites, especially benzoxazinoid derivatives. Potential copiotrophic and plant-beneficial taxa (e.g. Bacillus, Devosia, and Trichoderma) as well as functional genes (e.g. siderophore production, trehalose synthase, and ACC deaminase) were enriched in the CT rhizosphere, suggesting that tillage affected belowground plant–microorganism interactions. In addition, physiological stress markers were suppressed in CT winter wheat compared to MP. In summary, tillage practice was a major driver of crop performance, root deposits, and rhizosphere microbiome interactions, while the N-fertilization intensity was also relevant, but less important.
KW - 16S rRNA gene
KW - ITS Illumina amplicon sequencing
KW - mineral fertilization
KW - root exudates
KW - shotgun metagenome sequencing
KW - sustainable agriculture
U2 - 10.1093/femsec/fiae003
DO - 10.1093/femsec/fiae003
M3 - Journal article
C2 - 38224956
AN - SCOPUS:85184605254
VL - 100
JO - F E M S Microbiology Ecology
JF - F E M S Microbiology Ecology
SN - 0168-6496
IS - 2
M1 - fiae003
ER -
ID: 382993852