TY - JOUR

T1 - Molecular Epidemiology of HCV RNA Genotype-3 in Dhaka City, Bangladesh

AU - Rahman, Md Arifur

AU - Islam, Md Monirul

AU - Ali, Md Eunus

AU - Islam, Mohammad Ariful

AU - Afroze, Farhana

AU - Hossain, Mohammad Shamim

AU - Rus'd, Ahmed Abu

PY - 2023

Y1 - 2023

N2 - Hepatitis C virus (HCV) is a causative agent that causes chronic liver diseasesworldwide. It is a little, enclosed, single-stranded ribonucleic acid (RNA) virus. Therecognition of the pathogenic HCV genotype is critical for the remedy of its sufferers.The aim of this study was to identify the HCV RNA genotype to decide the correcttreatment of hepatitis C positive sufferers in Bangladesh. Blood samples were collectedfrom 390 individuals and isolated RNA (60 µg) from blood plasma. Extracted RNA wasused for quantitative HCV RNA, and complementary DNA (cDNA) was prepared bypolymerase chain reaction (PCR) conducted by reverse transcriptase enzyme. ThiscDNA amplified in multiplex by RT-PCR, which was performed with specific set ofprimers. The HCV RNA genotype was detected 297 of 390 patients. Of the 390 testsamples, 200 (51.28%) samples were from males and 190 (48.71%) were from females,with age ranging from 5 to 78 years. In all, 166 of 200 male samples and 131/190female samples were found positive for HCV. Of these 390 participants included in thestudy, 213 (54.61%) were identified as genotype 3 positive, 78 (20%) as genotype 1positive, 6 (1.53%) as genotype 6 positive, and the remaining 93 (23.85%) samples wereunclassified due to low/undetected viral load. In this study, we detected the highestpercentage (30.89%) of genotype 3 HCV in patients aged 51 to 60 years. The resultssuggested that genotype 3 HCV is frequently present in Bangladesh and it is usuallyresponses better to interferon therapy. However, genotype 1 and 6 HCV have also beenfound circulating in this country, which demands longer treatments and effectivecontrol measures.

AB - Hepatitis C virus (HCV) is a causative agent that causes chronic liver diseasesworldwide. It is a little, enclosed, single-stranded ribonucleic acid (RNA) virus. Therecognition of the pathogenic HCV genotype is critical for the remedy of its sufferers.The aim of this study was to identify the HCV RNA genotype to decide the correcttreatment of hepatitis C positive sufferers in Bangladesh. Blood samples were collectedfrom 390 individuals and isolated RNA (60 µg) from blood plasma. Extracted RNA wasused for quantitative HCV RNA, and complementary DNA (cDNA) was prepared bypolymerase chain reaction (PCR) conducted by reverse transcriptase enzyme. ThiscDNA amplified in multiplex by RT-PCR, which was performed with specific set ofprimers. The HCV RNA genotype was detected 297 of 390 patients. Of the 390 testsamples, 200 (51.28%) samples were from males and 190 (48.71%) were from females,with age ranging from 5 to 78 years. In all, 166 of 200 male samples and 131/190female samples were found positive for HCV. Of these 390 participants included in thestudy, 213 (54.61%) were identified as genotype 3 positive, 78 (20%) as genotype 1positive, 6 (1.53%) as genotype 6 positive, and the remaining 93 (23.85%) samples wereunclassified due to low/undetected viral load. In this study, we detected the highestpercentage (30.89%) of genotype 3 HCV in patients aged 51 to 60 years. The resultssuggested that genotype 3 HCV is frequently present in Bangladesh and it is usuallyresponses better to interferon therapy. However, genotype 1 and 6 HCV have also beenfound circulating in this country, which demands longer treatments and effectivecontrol measures.

U2 - 10.1055/s-0043-1771182

DO - 10.1055/s-0043-1771182

M3 - Journal article

C2 - 37565062

VL - 10

SP - 199

EP - 204

JO - Global Medical Genetics

JF - Global Medical Genetics

SN - 2699-9404

IS - 3

ER -