Streptomyces smaragdinus sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Streptomyces smaragdinus sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis. / Schwitalla, Jan W.; Benndorf, René; Martin, Karin; Vollmers, John; Kaster, Anne-Kristin; Wilhelm de Beer, Z.; Poulsen, Michael; Beemelmanns, Christine.
I: International Journal of Systematic and Evolutionary Microbiology, Bind 70, Nr. 11, 2020, s. 5806-5811.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Streptomyces smaragdinus sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis
AU - Schwitalla, Jan W.
AU - Benndorf, René
AU - Martin, Karin
AU - Vollmers, John
AU - Kaster, Anne-Kristin
AU - Wilhelm de Beer, Z.
AU - Poulsen, Michael
AU - Beemelmanns, Christine
PY - 2020
Y1 - 2020
N2 - The taxonomic position of a novel aerobic, Gram-positive actinobacteria, designated strain RB5T, was determined using a polyphasic approach. The strain, isolated from the gut of the fungus-farming termite Macrotermes natalensis, showed morphological, physiological and chemotaxonomic properties typical of the genus Streptomyces. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbour of RB5T was Streptomyces polyrhachis DSM 42102T (98.87%). DNA–DNA hybridization experiments between strain RB5T and S. polyrhachis DSM 42102T resulted in a value of 27.4% (26.8%). The cell wall of strain RB5T contained ll-diaminopimelic acid as the diagnostic amino acid. Mycolic acids and diagnostic sugars in whole-cell hydrolysates were not detected. The strain produced the following major phospholipids: diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and phosphatidylserine. The menaquinone profile showed hexa-and octahydrogenated menaquinones containing nine isoprene units [MK-9(H6 ) and MK-9(H8 )]. The strain exhibited a fatty acid profile containing the following major fatty acids: 12-methyltridecanoic acid (iso-C14:0 ) 12-methyltetradecanoic acid (anteiso-C15:0 ), 13-methyltetradecanoic acid (iso-C15:0 ) and 14-methylpentadecanoic acid (iso-C16:0 ). Here, we propose a novel species of the genus Streptomyces – Streptomyces smaragdinus with the type strain RB5T (=VKM Ac-2839T=NRRL B65539T).
AB - The taxonomic position of a novel aerobic, Gram-positive actinobacteria, designated strain RB5T, was determined using a polyphasic approach. The strain, isolated from the gut of the fungus-farming termite Macrotermes natalensis, showed morphological, physiological and chemotaxonomic properties typical of the genus Streptomyces. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbour of RB5T was Streptomyces polyrhachis DSM 42102T (98.87%). DNA–DNA hybridization experiments between strain RB5T and S. polyrhachis DSM 42102T resulted in a value of 27.4% (26.8%). The cell wall of strain RB5T contained ll-diaminopimelic acid as the diagnostic amino acid. Mycolic acids and diagnostic sugars in whole-cell hydrolysates were not detected. The strain produced the following major phospholipids: diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and phosphatidylserine. The menaquinone profile showed hexa-and octahydrogenated menaquinones containing nine isoprene units [MK-9(H6 ) and MK-9(H8 )]. The strain exhibited a fatty acid profile containing the following major fatty acids: 12-methyltridecanoic acid (iso-C14:0 ) 12-methyltetradecanoic acid (anteiso-C15:0 ), 13-methyltetradecanoic acid (iso-C15:0 ) and 14-methylpentadecanoic acid (iso-C16:0 ). Here, we propose a novel species of the genus Streptomyces – Streptomyces smaragdinus with the type strain RB5T (=VKM Ac-2839T=NRRL B65539T).
KW - Macrotermes natalensis
KW - Streptomyces
KW - Termite gut
U2 - 10.1099/ijsem.0.004478
DO - 10.1099/ijsem.0.004478
M3 - Journal article
C2 - 32969785
AN - SCOPUS:85096899631
VL - 70
SP - 5806
EP - 5811
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 11
ER -
ID: 252718846