The eclipse period of Escherichia coli

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

The eclipse period of Escherichia coli. / von Freiesleben, Ulrik; Krekling, Martin A.; Hansen, Flemming G.; Løbner-Olesen, Anders.

I: EMBO Journal, Bind 19, Nr. 22, 2000, s. 6240-6248.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

von Freiesleben, U, Krekling, MA, Hansen, FG & Løbner-Olesen, A 2000, 'The eclipse period of Escherichia coli', EMBO Journal, bind 19, nr. 22, s. 6240-6248. https://doi.org/10.1093/emboj/19.22.6240

APA

von Freiesleben, U., Krekling, M. A., Hansen, F. G., & Løbner-Olesen, A. (2000). The eclipse period of Escherichia coli. EMBO Journal, 19(22), 6240-6248. https://doi.org/10.1093/emboj/19.22.6240

Vancouver

von Freiesleben U, Krekling MA, Hansen FG, Løbner-Olesen A. The eclipse period of Escherichia coli. EMBO Journal. 2000;19(22):6240-6248. https://doi.org/10.1093/emboj/19.22.6240

Author

von Freiesleben, Ulrik ; Krekling, Martin A. ; Hansen, Flemming G. ; Løbner-Olesen, Anders. / The eclipse period of Escherichia coli. I: EMBO Journal. 2000 ; Bind 19, Nr. 22. s. 6240-6248.

Bibtex

@article{29532ea9cf0b47999ab021305a399877,
title = "The eclipse period of Escherichia coli",
abstract = "The minimal time between successive initiations on the same origin (the eclipse) in Escherichia coli was determined to be approximately 25-30 min. An inverse relationship was found between the length of the eclipse and the amount of Dam methyltransferase in the cell, indicating that the eclipse corresponds to the period of origin hemimethylation. The SeqA protein was absolutely required for the eclipse, and DnaA titration studies suggested that the SeqA protein prevented the binding of multiple DnaA molecules on oriC (initial complex formation). No correlation between the amount of SeqA and eclipse length was revealed, but increased SeqA levels affected chromosome partitioning and/or cell division. This was corroborated further by an aberrant nucleoid distribution in SeqA-deficient cells. We suggest that the SeqA protein's role in maintaining the eclipse is tied to a function in chromosome organization.",
keywords = "Bacterial Outer Membrane Proteins, Bacterial Proteins/genetics, Base Sequence, Cell Cycle, Cell Division, Chromosomes, Bacterial/metabolism, DNA Primers/genetics, DNA Replication, DNA-Binding Proteins/metabolism, Escherichia coli/cytology, Escherichia coli Proteins, Kinetics, Mutation, Origin Recognition Complex, Site-Specific DNA-Methyltransferase (Adenine-Specific)/metabolism, Transcription Factors, Viral Proteins",
author = "{von Freiesleben}, Ulrik and Krekling, {Martin A.} and Hansen, {Flemming G.} and Anders L{\o}bner-Olesen",
year = "2000",
doi = "10.1093/emboj/19.22.6240",
language = "English",
volume = "19",
pages = "6240--6248",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell",
number = "22",

}

RIS

TY - JOUR

T1 - The eclipse period of Escherichia coli

AU - von Freiesleben, Ulrik

AU - Krekling, Martin A.

AU - Hansen, Flemming G.

AU - Løbner-Olesen, Anders

PY - 2000

Y1 - 2000

N2 - The minimal time between successive initiations on the same origin (the eclipse) in Escherichia coli was determined to be approximately 25-30 min. An inverse relationship was found between the length of the eclipse and the amount of Dam methyltransferase in the cell, indicating that the eclipse corresponds to the period of origin hemimethylation. The SeqA protein was absolutely required for the eclipse, and DnaA titration studies suggested that the SeqA protein prevented the binding of multiple DnaA molecules on oriC (initial complex formation). No correlation between the amount of SeqA and eclipse length was revealed, but increased SeqA levels affected chromosome partitioning and/or cell division. This was corroborated further by an aberrant nucleoid distribution in SeqA-deficient cells. We suggest that the SeqA protein's role in maintaining the eclipse is tied to a function in chromosome organization.

AB - The minimal time between successive initiations on the same origin (the eclipse) in Escherichia coli was determined to be approximately 25-30 min. An inverse relationship was found between the length of the eclipse and the amount of Dam methyltransferase in the cell, indicating that the eclipse corresponds to the period of origin hemimethylation. The SeqA protein was absolutely required for the eclipse, and DnaA titration studies suggested that the SeqA protein prevented the binding of multiple DnaA molecules on oriC (initial complex formation). No correlation between the amount of SeqA and eclipse length was revealed, but increased SeqA levels affected chromosome partitioning and/or cell division. This was corroborated further by an aberrant nucleoid distribution in SeqA-deficient cells. We suggest that the SeqA protein's role in maintaining the eclipse is tied to a function in chromosome organization.

KW - Bacterial Outer Membrane Proteins

KW - Bacterial Proteins/genetics

KW - Base Sequence

KW - Cell Cycle

KW - Cell Division

KW - Chromosomes, Bacterial/metabolism

KW - DNA Primers/genetics

KW - DNA Replication

KW - DNA-Binding Proteins/metabolism

KW - Escherichia coli/cytology

KW - Escherichia coli Proteins

KW - Kinetics

KW - Mutation

KW - Origin Recognition Complex

KW - Site-Specific DNA-Methyltransferase (Adenine-Specific)/metabolism

KW - Transcription Factors

KW - Viral Proteins

U2 - 10.1093/emboj/19.22.6240

DO - 10.1093/emboj/19.22.6240

M3 - Journal article

C2 - 11080169

VL - 19

SP - 6240

EP - 6248

JO - E M B O Journal

JF - E M B O Journal

SN - 0261-4189

IS - 22

ER -

ID: 200972368