TY - JOUR

T1 - The gene for 2-phosphoglycolate phosphatase (gph) in Escherichia coli is located in the same operon as dam and at least five other diverse genes

AU - Lyngstadaas, Anita

AU - Løbner-Olesen, Anders

AU - Grelland, Ellen

AU - Boye, Erik

PY - 1999

Y1 - 1999

N2 - Downstream of the dam gene in the Escherichia coli genome the following three genes are located: first rpe, then a gene encoding a 27 kDa protein and finally trpS. Here we present evidence that the 27 kDa protein has 2-phosphoglycolate phosphatase activity, and we name the gene gph. Phosphoglycolate phosphatase is needed in autotrophic organisms performing the Calvin-Benson-Bassham (CBB) reductive pentose-phosphate cycle. E. coli is not capable of autotrophic growth and probably utilizes Gph activity for other function(s) than in the CBB cycle. We found no physiological effect of deleting gph and its function in E. coli remains unclear. The use of fusion plasmids, where lacZ was inserted into gph and trpS, and deletion derivatives of these fusion plasmids, showed that rpe, gph and trpS are all members of the dam-containing operon. A novel promoter was identified in the distal part of the dam gene. The operon, which contains aroK, aroB, urf74.3, dam, rpe, gph, and trpS, can be termed a superoperon, since it consists of (at least) seven apparently unrelated genes which are under complex regulatory control.

AB - Downstream of the dam gene in the Escherichia coli genome the following three genes are located: first rpe, then a gene encoding a 27 kDa protein and finally trpS. Here we present evidence that the 27 kDa protein has 2-phosphoglycolate phosphatase activity, and we name the gene gph. Phosphoglycolate phosphatase is needed in autotrophic organisms performing the Calvin-Benson-Bassham (CBB) reductive pentose-phosphate cycle. E. coli is not capable of autotrophic growth and probably utilizes Gph activity for other function(s) than in the CBB cycle. We found no physiological effect of deleting gph and its function in E. coli remains unclear. The use of fusion plasmids, where lacZ was inserted into gph and trpS, and deletion derivatives of these fusion plasmids, showed that rpe, gph and trpS are all members of the dam-containing operon. A novel promoter was identified in the distal part of the dam gene. The operon, which contains aroK, aroB, urf74.3, dam, rpe, gph, and trpS, can be termed a superoperon, since it consists of (at least) seven apparently unrelated genes which are under complex regulatory control.

KW - Artificial Gene Fusion

KW - Base Sequence

KW - DNA, Bacterial

KW - Escherichia coli/enzymology

KW - Escherichia coli Proteins

KW - Genes, Bacterial

KW - Lac Operon

KW - Molecular Sequence Data

KW - Operon

KW - Phosphoric Monoester Hydrolases/genetics

KW - Plasmids

KW - Promoter Regions, Genetic

KW - Sequence Deletion

KW - Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics

KW - Transcription, Genetic

U2 - 10.1016/S0304-4165(99)00146-4

DO - 10.1016/S0304-4165(99)00146-4

M3 - Journal article

C2 - 10572959

VL - 1472

SP - 376

EP - 384

JO - B B A - General Subjects

JF - B B A - General Subjects

SN - 0304-4165

IS - 1-2

ER -