The Microprocessor controls the activity of mammalian retrotransposons

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Standard

The Microprocessor controls the activity of mammalian retrotransposons. / Heras, Sara R.; Macias, Sara; Plass, Mireya; Fernandez, Noemí; Cano, David; Eyras, Eduardo; Garcia-Perez, José L.; Cáceres, Javier F.

I: Nature Structural and Molecular Biology, Bind 20, Nr. 10, 2013, s. 1173-1181.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Heras, SR, Macias, S, Plass, M, Fernandez, N, Cano, D, Eyras, E, Garcia-Perez, JL & Cáceres, JF 2013, 'The Microprocessor controls the activity of mammalian retrotransposons', Nature Structural and Molecular Biology, bind 20, nr. 10, s. 1173-1181. https://doi.org/10.1038/nsmb.2658

APA

Heras, S. R., Macias, S., Plass, M., Fernandez, N., Cano, D., Eyras, E., Garcia-Perez, J. L., & Cáceres, J. F. (2013). The Microprocessor controls the activity of mammalian retrotransposons. Nature Structural and Molecular Biology, 20(10), 1173-1181. https://doi.org/10.1038/nsmb.2658

Vancouver

Heras SR, Macias S, Plass M, Fernandez N, Cano D, Eyras E o.a. The Microprocessor controls the activity of mammalian retrotransposons. Nature Structural and Molecular Biology. 2013;20(10):1173-1181. https://doi.org/10.1038/nsmb.2658

Author

Heras, Sara R. ; Macias, Sara ; Plass, Mireya ; Fernandez, Noemí ; Cano, David ; Eyras, Eduardo ; Garcia-Perez, José L. ; Cáceres, Javier F. / The Microprocessor controls the activity of mammalian retrotransposons. I: Nature Structural and Molecular Biology. 2013 ; Bind 20, Nr. 10. s. 1173-1181.

Bibtex

@article{334d65b36e9849aa990def3868ab8e2e,
title = "The Microprocessor controls the activity of mammalian retrotransposons",
abstract = "More than half of the human genome is made of transposable elements whose ongoing mobilization is a driving force in genetic diversity; however, little is known about how the host regulates their activity. Here, we show that the Microprocessor (Drosha-DGCR8), which is required for microRNA biogenesis, also recognizes and binds RNAs derived from human long interspersed element 1 (LINE-1), Alu and SVA retrotransposons. Expression analyses demonstrate that cells lacking a functional Microprocessor accumulate LINE-1 mRNA and encoded proteins. Furthermore, we show that structured regions of the LINE-1 mRNA can be cleaved in vitro by Drosha. Additionally, we used a cell culture-based assay to show that the Microprocessor negatively regulates LINE-1 and Alu retrotransposition in vivo. Altogether, these data reveal a new role for the Microprocessor as a post-transcriptional repressor of mammalian retrotransposons and a defender of human genome integrity.",
author = "Heras, {Sara R.} and Sara Macias and Mireya Plass and Noem{\'i} Fernandez and David Cano and Eduardo Eyras and Garcia-Perez, {Jos{\'e} L.} and C{\'a}ceres, {Javier F.}",
year = "2013",
doi = "10.1038/nsmb.2658",
language = "English",
volume = "20",
pages = "1173--1181",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "nature publishing group",
number = "10",

}

RIS

TY - JOUR

T1 - The Microprocessor controls the activity of mammalian retrotransposons

AU - Heras, Sara R.

AU - Macias, Sara

AU - Plass, Mireya

AU - Fernandez, Noemí

AU - Cano, David

AU - Eyras, Eduardo

AU - Garcia-Perez, José L.

AU - Cáceres, Javier F.

PY - 2013

Y1 - 2013

N2 - More than half of the human genome is made of transposable elements whose ongoing mobilization is a driving force in genetic diversity; however, little is known about how the host regulates their activity. Here, we show that the Microprocessor (Drosha-DGCR8), which is required for microRNA biogenesis, also recognizes and binds RNAs derived from human long interspersed element 1 (LINE-1), Alu and SVA retrotransposons. Expression analyses demonstrate that cells lacking a functional Microprocessor accumulate LINE-1 mRNA and encoded proteins. Furthermore, we show that structured regions of the LINE-1 mRNA can be cleaved in vitro by Drosha. Additionally, we used a cell culture-based assay to show that the Microprocessor negatively regulates LINE-1 and Alu retrotransposition in vivo. Altogether, these data reveal a new role for the Microprocessor as a post-transcriptional repressor of mammalian retrotransposons and a defender of human genome integrity.

AB - More than half of the human genome is made of transposable elements whose ongoing mobilization is a driving force in genetic diversity; however, little is known about how the host regulates their activity. Here, we show that the Microprocessor (Drosha-DGCR8), which is required for microRNA biogenesis, also recognizes and binds RNAs derived from human long interspersed element 1 (LINE-1), Alu and SVA retrotransposons. Expression analyses demonstrate that cells lacking a functional Microprocessor accumulate LINE-1 mRNA and encoded proteins. Furthermore, we show that structured regions of the LINE-1 mRNA can be cleaved in vitro by Drosha. Additionally, we used a cell culture-based assay to show that the Microprocessor negatively regulates LINE-1 and Alu retrotransposition in vivo. Altogether, these data reveal a new role for the Microprocessor as a post-transcriptional repressor of mammalian retrotransposons and a defender of human genome integrity.

U2 - 10.1038/nsmb.2658

DO - 10.1038/nsmb.2658

M3 - Journal article

C2 - 23995758

VL - 20

SP - 1173

EP - 1181

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

SN - 1545-9993

IS - 10

ER -

ID: 50802314