The pat1 protein kinase controls transcription of the mating-type genes in fission yeast
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The pat1 protein kinase controls transcription of the mating-type genes in fission yeast. / Nielsen, O; Egel, R; Nielsen, Olaf.
I: E M B O Journal, Bind 9, Nr. 5, 01.05.1990, s. 1401-6.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The pat1 protein kinase controls transcription of the mating-type genes in fission yeast
AU - Nielsen, O
AU - Egel, R
AU - Nielsen, Olaf
PY - 1990/5/1
Y1 - 1990/5/1
N2 - The developmental programme of fission yeast brings about a transition from mitotic cell division to the dormant state of ascospores. In response to nitrogen starvation, two cells of opposite mating type conjugate to form a diploid zygote, which then undergoes meiosis and sporulation. This differentiation process is characterized by a transcriptional induction of the mating-type genes. Conjugation can also be induced in pat1-ts mutants by a shift to a semi-permissive temperature. The pat1 gene encodes a protein kinase, which also functions further downstream in the developmental pathway controlling entry into meiosis. We have analysed transcriptional induction of mating-type genes in various strains--with and without a pat1-ts allele. In wild-type cells of P-mating type derepression occurs in two rounds. First, the mat1-Pc gene is induced in response to nitrogen starvation. Mutants in the map1 gene are defective in this process. In the following step the mat1-Pm gene is expressed in response to a pheromone signal generated by cells of M mating type. Both these controls are derepressed in the pat1-ts mutant at semipermissive temperature. Previous work has established that expression of the mating-type genes in the zygote leads to complete loss of pat1 protein kinase activity causing entry into meiosis. Thus, pat1 can promote its own inactivation. We suggest a model according to which a stepwise inactivation of pat1 leads to sequential derepression of the processes of conjugation and meiosis.
AB - The developmental programme of fission yeast brings about a transition from mitotic cell division to the dormant state of ascospores. In response to nitrogen starvation, two cells of opposite mating type conjugate to form a diploid zygote, which then undergoes meiosis and sporulation. This differentiation process is characterized by a transcriptional induction of the mating-type genes. Conjugation can also be induced in pat1-ts mutants by a shift to a semi-permissive temperature. The pat1 gene encodes a protein kinase, which also functions further downstream in the developmental pathway controlling entry into meiosis. We have analysed transcriptional induction of mating-type genes in various strains--with and without a pat1-ts allele. In wild-type cells of P-mating type derepression occurs in two rounds. First, the mat1-Pc gene is induced in response to nitrogen starvation. Mutants in the map1 gene are defective in this process. In the following step the mat1-Pm gene is expressed in response to a pheromone signal generated by cells of M mating type. Both these controls are derepressed in the pat1-ts mutant at semipermissive temperature. Previous work has established that expression of the mating-type genes in the zygote leads to complete loss of pat1 protein kinase activity causing entry into meiosis. Thus, pat1 can promote its own inactivation. We suggest a model according to which a stepwise inactivation of pat1 leads to sequential derepression of the processes of conjugation and meiosis.
KW - Conjugation, Genetic
KW - Fungal Proteins
KW - Gene Expression Regulation, Fungal
KW - Genes, Fungal
KW - Genes, Mating Type, Fungal
KW - Meiosis
KW - Models, Biological
KW - Protein Kinases
KW - Repressor Proteins
KW - Saccharomycetales
KW - Schizosaccharomyces
KW - Temperature
KW - Transcription Factors
KW - Transcription, Genetic
M3 - Journal article
C2 - 2328719
VL - 9
SP - 1401
EP - 1406
JO - E M B O Journal
JF - E M B O Journal
SN - 0261-4189
IS - 5
ER -
ID: 33577636