The T-shirt microbiome is distinct between individuals and shaped by washing and fabric type
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
The T-shirt microbiome is distinct between individuals and shaped by washing and fabric type. / Sterndorff, Eva Baggesgaard; Russel, Jakob; Jakobsen, Jonas; Mortensen, Martin Steen; Gori, Klaus; Herschend, Jakob; Burmølle, Mette.
I: Environmental Research, Bind 185, 109449, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The T-shirt microbiome is distinct between individuals and shaped by washing and fabric type
AU - Sterndorff, Eva Baggesgaard
AU - Russel, Jakob
AU - Jakobsen, Jonas
AU - Mortensen, Martin Steen
AU - Gori, Klaus
AU - Herschend, Jakob
AU - Burmølle, Mette
PY - 2020
Y1 - 2020
N2 - Activity of the microbial population in clothing causes unpleasant odor and textile deterioration. However, little is known about how the textile microbial community is shaped. In this study, we developed a method for extracting DNA from small amounts of detergent-washed clothing, and applied it to both worn and unworn, washed and unwashed cotton and polyester samples of the axillary region of T-shirts from 10 male subjects. The combined application of 16S rRNA gene amplicon sequencing and quantitative PCR allowed us to estimate the absolute abundances of bacteria in the samples. We found that the T-shirt microbiome was highly individual, both in composition, diversity and microbial biomass. Fabric type was influential where Acinetobacter was more abundant in cotton. Intriguingly, unworn cotton T-shirts had a native microbiome dominated by Acinetobacter, whereas unworn polyester had no detectable bacterial microbiome. The native textile microbiome did not seem to have any effect on the microbial composition emerging from wearing the garment. Surprisingly, washing in mild detergent had only minor effects on the composition and biomass of the microbial community, and only few Amplicon Sequence Variants (ASV)s were found to decrease in abundance after washing. Individual variations between test subjects shaped the microbial community more than the type of fabric or wash with detergent. The individuality of T-shirt microbiomes and specificity of the washing procedure suggests that personalized laundry regimes could be applied to increase efficient removal of undesired bacteria.
AB - Activity of the microbial population in clothing causes unpleasant odor and textile deterioration. However, little is known about how the textile microbial community is shaped. In this study, we developed a method for extracting DNA from small amounts of detergent-washed clothing, and applied it to both worn and unworn, washed and unwashed cotton and polyester samples of the axillary region of T-shirts from 10 male subjects. The combined application of 16S rRNA gene amplicon sequencing and quantitative PCR allowed us to estimate the absolute abundances of bacteria in the samples. We found that the T-shirt microbiome was highly individual, both in composition, diversity and microbial biomass. Fabric type was influential where Acinetobacter was more abundant in cotton. Intriguingly, unworn cotton T-shirts had a native microbiome dominated by Acinetobacter, whereas unworn polyester had no detectable bacterial microbiome. The native textile microbiome did not seem to have any effect on the microbial composition emerging from wearing the garment. Surprisingly, washing in mild detergent had only minor effects on the composition and biomass of the microbial community, and only few Amplicon Sequence Variants (ASV)s were found to decrease in abundance after washing. Individual variations between test subjects shaped the microbial community more than the type of fabric or wash with detergent. The individuality of T-shirt microbiomes and specificity of the washing procedure suggests that personalized laundry regimes could be applied to increase efficient removal of undesired bacteria.
KW - 16S rRNA gene sequencing
KW - Bacteria
KW - Biofilm
KW - Clothing
KW - Detergent
KW - Microbiome
KW - Textile
U2 - 10.1016/j.envres.2020.109449
DO - 10.1016/j.envres.2020.109449
M3 - Journal article
C2 - 32278157
AN - SCOPUS:85082750328
VL - 185
JO - Environmental Research
JF - Environmental Research
SN - 0013-9351
M1 - 109449
ER -
ID: 239855829