Transmembrane Protein Aptamer Induces Cooperative Signaling by the EPO Receptor and the Cytokine Receptor β-Common Subunit

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Standard

Transmembrane Protein Aptamer Induces Cooperative Signaling by the EPO Receptor and the Cytokine Receptor β-Common Subunit. / He, Li; Cohen, Emily B.; Edwards, Anne P. B.; Xavier-Ferrucio, Juliana; Bugge, Katrine; Federman, Ross S.; Absher, Devin; Myers, Richard M.; Kragelund, Birthe B.; Krause, Diane S.; DiMaio, Daniel.

I: iScience, Bind 17, 2019, s. 167-181.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

He, L, Cohen, EB, Edwards, APB, Xavier-Ferrucio, J, Bugge, K, Federman, RS, Absher, D, Myers, RM, Kragelund, BB, Krause, DS & DiMaio, D 2019, 'Transmembrane Protein Aptamer Induces Cooperative Signaling by the EPO Receptor and the Cytokine Receptor β-Common Subunit', iScience, bind 17, s. 167-181. https://doi.org/10.1016/j.isci.2019.06.027

APA

He, L., Cohen, E. B., Edwards, A. P. B., Xavier-Ferrucio, J., Bugge, K., Federman, R. S., Absher, D., Myers, R. M., Kragelund, B. B., Krause, D. S., & DiMaio, D. (2019). Transmembrane Protein Aptamer Induces Cooperative Signaling by the EPO Receptor and the Cytokine Receptor β-Common Subunit. iScience, 17, 167-181. https://doi.org/10.1016/j.isci.2019.06.027

Vancouver

He L, Cohen EB, Edwards APB, Xavier-Ferrucio J, Bugge K, Federman RS o.a. Transmembrane Protein Aptamer Induces Cooperative Signaling by the EPO Receptor and the Cytokine Receptor β-Common Subunit. iScience. 2019;17:167-181. https://doi.org/10.1016/j.isci.2019.06.027

Author

He, Li ; Cohen, Emily B. ; Edwards, Anne P. B. ; Xavier-Ferrucio, Juliana ; Bugge, Katrine ; Federman, Ross S. ; Absher, Devin ; Myers, Richard M. ; Kragelund, Birthe B. ; Krause, Diane S. ; DiMaio, Daniel. / Transmembrane Protein Aptamer Induces Cooperative Signaling by the EPO Receptor and the Cytokine Receptor β-Common Subunit. I: iScience. 2019 ; Bind 17. s. 167-181.

Bibtex

@article{77a9d27f8c1249588934c2e982f29a86,
title = "Transmembrane Protein Aptamer Induces Cooperative Signaling by the EPO Receptor and the Cytokine Receptor β-Common Subunit",
abstract = "The erythropoietin receptor (EPOR) plays an essential role in erythropoiesis and other cellular processes by forming distinct signaling complexes composed of EPOR homodimers or hetero-oligomers between the EPOR and another receptor, but the mechanism of heteroreceptor assembly and signaling is poorly understood. We report here a 46-residue, artificial transmembrane protein aptamer, designated ELI-3, that binds and activates the EPOR and induces growth factor independence in murine BaF3 cells expressing the EPOR. ELI-3 requires the transmembrane domain and JAK2-binding sites of the EPOR for activity, but not the cytoplasmic tyrosines that mediate canonical EPOR signaling. Instead, ELI-3-induced proliferation and activation of JAK/STAT signaling requires the transmembrane and cytoplasmic domains of the cytokine receptor β-common subunit (βcR) in addition to the EPOR. Moreover, ELI-3 fails to induce erythroid differentiation of primary human hematopoietic progenitor cells but inhibits nonhematopoietic cell death induced by serum withdrawal.",
author = "Li He and Cohen, {Emily B.} and Edwards, {Anne P. B.} and Juliana Xavier-Ferrucio and Katrine Bugge and Federman, {Ross S.} and Devin Absher and Myers, {Richard M.} and Kragelund, {Birthe B.} and Krause, {Diane S.} and Daniel DiMaio",
note = "Copyright {\textcopyright} 2019 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2019",
doi = "10.1016/j.isci.2019.06.027",
language = "English",
volume = "17",
pages = "167--181",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Transmembrane Protein Aptamer Induces Cooperative Signaling by the EPO Receptor and the Cytokine Receptor β-Common Subunit

AU - He, Li

AU - Cohen, Emily B.

AU - Edwards, Anne P. B.

AU - Xavier-Ferrucio, Juliana

AU - Bugge, Katrine

AU - Federman, Ross S.

AU - Absher, Devin

AU - Myers, Richard M.

AU - Kragelund, Birthe B.

AU - Krause, Diane S.

AU - DiMaio, Daniel

N1 - Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2019

Y1 - 2019

N2 - The erythropoietin receptor (EPOR) plays an essential role in erythropoiesis and other cellular processes by forming distinct signaling complexes composed of EPOR homodimers or hetero-oligomers between the EPOR and another receptor, but the mechanism of heteroreceptor assembly and signaling is poorly understood. We report here a 46-residue, artificial transmembrane protein aptamer, designated ELI-3, that binds and activates the EPOR and induces growth factor independence in murine BaF3 cells expressing the EPOR. ELI-3 requires the transmembrane domain and JAK2-binding sites of the EPOR for activity, but not the cytoplasmic tyrosines that mediate canonical EPOR signaling. Instead, ELI-3-induced proliferation and activation of JAK/STAT signaling requires the transmembrane and cytoplasmic domains of the cytokine receptor β-common subunit (βcR) in addition to the EPOR. Moreover, ELI-3 fails to induce erythroid differentiation of primary human hematopoietic progenitor cells but inhibits nonhematopoietic cell death induced by serum withdrawal.

AB - The erythropoietin receptor (EPOR) plays an essential role in erythropoiesis and other cellular processes by forming distinct signaling complexes composed of EPOR homodimers or hetero-oligomers between the EPOR and another receptor, but the mechanism of heteroreceptor assembly and signaling is poorly understood. We report here a 46-residue, artificial transmembrane protein aptamer, designated ELI-3, that binds and activates the EPOR and induces growth factor independence in murine BaF3 cells expressing the EPOR. ELI-3 requires the transmembrane domain and JAK2-binding sites of the EPOR for activity, but not the cytoplasmic tyrosines that mediate canonical EPOR signaling. Instead, ELI-3-induced proliferation and activation of JAK/STAT signaling requires the transmembrane and cytoplasmic domains of the cytokine receptor β-common subunit (βcR) in addition to the EPOR. Moreover, ELI-3 fails to induce erythroid differentiation of primary human hematopoietic progenitor cells but inhibits nonhematopoietic cell death induced by serum withdrawal.

U2 - 10.1016/j.isci.2019.06.027

DO - 10.1016/j.isci.2019.06.027

M3 - Journal article

C2 - 31279934

VL - 17

SP - 167

EP - 181

JO - iScience

JF - iScience

SN - 2589-0042

ER -

ID: 225279032