Isolation and life cycle characterization of lytic viruses infecting heterotrophic bacteria and cyanobacteria

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Isolation and life cycle characterization of lytic viruses infecting heterotrophic bacteria and cyanobacteria. / Middelboe, Mathias; Chan, Amy; Bertelsen, Sif Koldborg.

Manual of aquatic viral ecology. ed. / S. W. Wilhelm; M. G. Weinbauer; C. A. Suttle. Waco, Tex : American Society of Limnology and Oceanography, Inc., 2010. p. 118-33.

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Middelboe, M, Chan, A & Bertelsen, SK 2010, Isolation and life cycle characterization of lytic viruses infecting heterotrophic bacteria and cyanobacteria. in SW Wilhelm, MG Weinbauer & CA Suttle (eds), Manual of aquatic viral ecology. American Society of Limnology and Oceanography, Inc., Waco, Tex, pp. 118-33. https://doi.org/10.4319/mave.2010.978-0-9845591-0-7.118

APA

Middelboe, M., Chan, A., & Bertelsen, S. K. (2010). Isolation and life cycle characterization of lytic viruses infecting heterotrophic bacteria and cyanobacteria. In S. W. Wilhelm, M. G. Weinbauer, & C. A. Suttle (Eds.), Manual of aquatic viral ecology (pp. 118-33). American Society of Limnology and Oceanography, Inc.. https://doi.org/10.4319/mave.2010.978-0-9845591-0-7.118

Vancouver

Middelboe M, Chan A, Bertelsen SK. Isolation and life cycle characterization of lytic viruses infecting heterotrophic bacteria and cyanobacteria. In Wilhelm SW, Weinbauer MG, Suttle CA, editors, Manual of aquatic viral ecology. Waco, Tex: American Society of Limnology and Oceanography, Inc. 2010. p. 118-33 https://doi.org/10.4319/mave.2010.978-0-9845591-0-7.118

Author

Middelboe, Mathias ; Chan, Amy ; Bertelsen, Sif Koldborg. / Isolation and life cycle characterization of lytic viruses infecting heterotrophic bacteria and cyanobacteria. Manual of aquatic viral ecology. editor / S. W. Wilhelm ; M. G. Weinbauer ; C. A. Suttle. Waco, Tex : American Society of Limnology and Oceanography, Inc., 2010. pp. 118-33

Bibtex

@inbook{34aef9c0fa0011de825d000ea68e967b,
title = "Isolation and life cycle characterization of lytic viruses infecting heterotrophic bacteria and cyanobacteria",
abstract = "Basic knowledge on viruses infecting heterotrophic bacteria and cyanobacteria is key to future progress in understanding the role of viruses in aquatic systems and the influence of virus–host interactions on microbial mortality, biogeochemical cycles, and genetic exchange. Such studies require the isolation, propagation, and purification of host–virus systems. This contribution presents some of the most widely used methodological approaches for isolation and purification of bacteriophages and cyanophages, the first step in detailed studies of virus–host interactions and viral genetic composition, and discusses the applications and limitations of different isolation procedures. Most work on phage isolation has been carried out with aerobic heterotrophic bacteria and cyanobacteria, culturable both on agar plates and in enriched liquid cultures. The procedures presented here are limited to lytic viruses infecting such hosts. In addition to the isolation procedures, methods for life cycle characterization (one-step growth experiments) of bacteriophages and cyanophages are described. Finally, limitations and drawbacks of the proposed methods are assessed and discussed",
author = "Mathias Middelboe and Amy Chan and Bertelsen, {Sif Koldborg}",
year = "2010",
doi = "10.4319/mave.2010.978-0-9845591-0-7.118",
language = "English",
pages = "118--33",
editor = "Wilhelm, {S. W. } and Weinbauer, {M. G. } and Suttle, {C. A. }",
booktitle = "Manual of aquatic viral ecology",
publisher = "American Society of Limnology and Oceanography, Inc.",

}

RIS

TY - CHAP

T1 - Isolation and life cycle characterization of lytic viruses infecting heterotrophic bacteria and cyanobacteria

AU - Middelboe, Mathias

AU - Chan, Amy

AU - Bertelsen, Sif Koldborg

PY - 2010

Y1 - 2010

N2 - Basic knowledge on viruses infecting heterotrophic bacteria and cyanobacteria is key to future progress in understanding the role of viruses in aquatic systems and the influence of virus–host interactions on microbial mortality, biogeochemical cycles, and genetic exchange. Such studies require the isolation, propagation, and purification of host–virus systems. This contribution presents some of the most widely used methodological approaches for isolation and purification of bacteriophages and cyanophages, the first step in detailed studies of virus–host interactions and viral genetic composition, and discusses the applications and limitations of different isolation procedures. Most work on phage isolation has been carried out with aerobic heterotrophic bacteria and cyanobacteria, culturable both on agar plates and in enriched liquid cultures. The procedures presented here are limited to lytic viruses infecting such hosts. In addition to the isolation procedures, methods for life cycle characterization (one-step growth experiments) of bacteriophages and cyanophages are described. Finally, limitations and drawbacks of the proposed methods are assessed and discussed

AB - Basic knowledge on viruses infecting heterotrophic bacteria and cyanobacteria is key to future progress in understanding the role of viruses in aquatic systems and the influence of virus–host interactions on microbial mortality, biogeochemical cycles, and genetic exchange. Such studies require the isolation, propagation, and purification of host–virus systems. This contribution presents some of the most widely used methodological approaches for isolation and purification of bacteriophages and cyanophages, the first step in detailed studies of virus–host interactions and viral genetic composition, and discusses the applications and limitations of different isolation procedures. Most work on phage isolation has been carried out with aerobic heterotrophic bacteria and cyanobacteria, culturable both on agar plates and in enriched liquid cultures. The procedures presented here are limited to lytic viruses infecting such hosts. In addition to the isolation procedures, methods for life cycle characterization (one-step growth experiments) of bacteriophages and cyanophages are described. Finally, limitations and drawbacks of the proposed methods are assessed and discussed

U2 - 10.4319/mave.2010.978-0-9845591-0-7.118

DO - 10.4319/mave.2010.978-0-9845591-0-7.118

M3 - Book chapter

SP - 118

EP - 133

BT - Manual of aquatic viral ecology

A2 - Wilhelm, S. W.

A2 - Weinbauer, M. G.

A2 - Suttle, C. A.

PB - American Society of Limnology and Oceanography, Inc.

CY - Waco, Tex

ER -

ID: 16786724