Rice leaf hydrophobicity and gas films are conferred by a wax synthesis gene (LGF1) and contribute to flood tolerance
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Rice leaf hydrophobicity and gas films are conferred by a wax synthesis gene (LGF1) and contribute to flood tolerance. / Kurokawa, Yusuke; Nagai, Keisuke; Hung, Phung Danh; Shimazaki, Kousuke; Qu, Huangqi; Mori, Yoshinao; Toda, Yusuke; Kuroha, Takeshi; Hayashi, Nagao; Aiga, Saori; Itoh, Jun-ichi; Yoshimura, Atsushi; Sasaki-Sekimoto, Yuko; Ohta, Hiroyuki; Shimojima, Mie; Malik, Al Imran; Pedersen, Ole; Colmer, Timothy David; Ashikari, Motoyuki.
In: New Phytologist, Vol. 218, No. 4, 2018, p. 1558-1569.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Rice leaf hydrophobicity and gas films are conferred by a wax synthesis gene (LGF1) and contribute to flood tolerance
AU - Kurokawa, Yusuke
AU - Nagai, Keisuke
AU - Hung, Phung Danh
AU - Shimazaki, Kousuke
AU - Qu, Huangqi
AU - Mori, Yoshinao
AU - Toda, Yusuke
AU - Kuroha, Takeshi
AU - Hayashi, Nagao
AU - Aiga, Saori
AU - Itoh, Jun-ichi
AU - Yoshimura, Atsushi
AU - Sasaki-Sekimoto, Yuko
AU - Ohta, Hiroyuki
AU - Shimojima, Mie
AU - Malik, Al Imran
AU - Pedersen, Ole
AU - Colmer, Timothy David
AU - Ashikari, Motoyuki
N1 - © 2018 The Authors New Phytologist © 2018 New Phytologist Trust.
PY - 2018
Y1 - 2018
N2 - Floods impede gas (O2and CO2) exchange between plants and the environment. A mechanism to enhance plant gas exchange under water comprises gas films on hydrophobic leaves, but the genetic regulation of this mechanism is unknown. We used a rice mutant (dripping wet leaf 7, drp7) which does not retain gas films on leaves, and its wild-type (Kinmaze), in gene discovery for this trait. Gene complementation was tested in transgenic lines. Functional properties of leaves as related to gas film retention and underwater photosynthesis were evaluated. Leaf Gas Film 1 (LGF1) was identified as the gene determining leaf gas films. LGF1 regulates C30 primary alcohol synthesis, which is necessary for abundant epicuticular wax platelets, leaf hydrophobicity and gas films on submerged leaves. This trait enhanced underwater photosynthesis 8.2-fold and contributes to submergence tolerance. Gene function was verified by a complementation test of LGF1 expressed in the drp7 mutant background, which restored C30 primary alcohol synthesis, wax platelet abundance, leaf hydrophobicity, gas film retention, and underwater photosynthesis. The discovery of LGF1 provides an opportunity to better understand variation amongst rice genotypes for gas film retention ability and to target various alleles in breeding for improved submergence tolerance for yield stability in flood-prone areas.
AB - Floods impede gas (O2and CO2) exchange between plants and the environment. A mechanism to enhance plant gas exchange under water comprises gas films on hydrophobic leaves, but the genetic regulation of this mechanism is unknown. We used a rice mutant (dripping wet leaf 7, drp7) which does not retain gas films on leaves, and its wild-type (Kinmaze), in gene discovery for this trait. Gene complementation was tested in transgenic lines. Functional properties of leaves as related to gas film retention and underwater photosynthesis were evaluated. Leaf Gas Film 1 (LGF1) was identified as the gene determining leaf gas films. LGF1 regulates C30 primary alcohol synthesis, which is necessary for abundant epicuticular wax platelets, leaf hydrophobicity and gas films on submerged leaves. This trait enhanced underwater photosynthesis 8.2-fold and contributes to submergence tolerance. Gene function was verified by a complementation test of LGF1 expressed in the drp7 mutant background, which restored C30 primary alcohol synthesis, wax platelet abundance, leaf hydrophobicity, gas film retention, and underwater photosynthesis. The discovery of LGF1 provides an opportunity to better understand variation amongst rice genotypes for gas film retention ability and to target various alleles in breeding for improved submergence tolerance for yield stability in flood-prone areas.
U2 - 10.1111/nph.15070
DO - 10.1111/nph.15070
M3 - Journal article
C2 - 29498045
VL - 218
SP - 1558
EP - 1569
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 4
ER -
ID: 191905361