Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages
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Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages. / Parra, Boris; Lutz, Veronika T.; Brøndsted, Lone; Carmona, Javiera L.; Palomo, Alejandro; Nesme, Joseph; Van Hung Le, Vuong; Smets, Barth F.; Dechesne, Arnaud.
In: Microbial Ecology, Vol. 87, No. 1, 85, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages
AU - Parra, Boris
AU - Lutz, Veronika T.
AU - Brøndsted, Lone
AU - Carmona, Javiera L.
AU - Palomo, Alejandro
AU - Nesme, Joseph
AU - Van Hung Le, Vuong
AU - Smets, Barth F.
AU - Dechesne, Arnaud
N1 - Publisher Copyright: © The Author(s) 2024.
PY - 2024
Y1 - 2024
N2 - Antimicrobial resistance (AMR) is a major public health threat, exacerbated by the ability of bacteria to rapidly disseminate antimicrobial resistance genes (ARG). Since conjugative plasmids of the incompatibility group P (IncP) are ubiquitous mobile genetic elements that often carry ARG and are broad-host-range, they are important targets to prevent the dissemination of AMR. Plasmid-dependent phages infect plasmid-carrying bacteria by recognizing components of the conjugative secretion system as receptors. We sought to isolate plasmid-dependent phages from wastewater using an avirulent strain of Salmonella enterica carrying the conjugative IncP plasmid pKJK5. Irrespective of the site, we only obtained bacteriophages belonging to the genus Alphatectivirus. Eleven isolates were sequenced, their genomes analyzed, and their host range established using S. enterica, Escherichia coli, and Pseudomonas putida carrying diverse conjugative plasmids. We confirmed that Alphatectivirus are abundant in domestic and hospital wastewater using culture-dependent and culture-independent approaches. However, these results are not consistent with their low or undetectable occurrence in metagenomes. Therefore, overall, our results emphasize the importance of performing phage isolation to uncover diversity, especially considering the potential of plasmid-dependent phages to reduce the spread of ARG carried by conjugative plasmids, and to help combat the AMR crisis.
AB - Antimicrobial resistance (AMR) is a major public health threat, exacerbated by the ability of bacteria to rapidly disseminate antimicrobial resistance genes (ARG). Since conjugative plasmids of the incompatibility group P (IncP) are ubiquitous mobile genetic elements that often carry ARG and are broad-host-range, they are important targets to prevent the dissemination of AMR. Plasmid-dependent phages infect plasmid-carrying bacteria by recognizing components of the conjugative secretion system as receptors. We sought to isolate plasmid-dependent phages from wastewater using an avirulent strain of Salmonella enterica carrying the conjugative IncP plasmid pKJK5. Irrespective of the site, we only obtained bacteriophages belonging to the genus Alphatectivirus. Eleven isolates were sequenced, their genomes analyzed, and their host range established using S. enterica, Escherichia coli, and Pseudomonas putida carrying diverse conjugative plasmids. We confirmed that Alphatectivirus are abundant in domestic and hospital wastewater using culture-dependent and culture-independent approaches. However, these results are not consistent with their low or undetectable occurrence in metagenomes. Therefore, overall, our results emphasize the importance of performing phage isolation to uncover diversity, especially considering the potential of plasmid-dependent phages to reduce the spread of ARG carried by conjugative plasmids, and to help combat the AMR crisis.
KW - Antimicrobial resistance
KW - Bacteria
KW - Bacteriophages
KW - Conjugation
KW - Horizontal gene transfer
KW - Plasmid
KW - Virus
U2 - 10.1007/s00248-024-02401-3
DO - 10.1007/s00248-024-02401-3
M3 - Journal article
C2 - 38935220
AN - SCOPUS:85197202770
VL - 87
JO - Microbial Ecology
JF - Microbial Ecology
SN - 0095-3628
IS - 1
M1 - 85
ER -
ID: 398076968