A Bacterial Genome and Culture Collection of Gut Microbial in Weanling Piglet
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A Bacterial Genome and Culture Collection of Gut Microbial in Weanling Piglet. / Dong, Bo; Lin, Xiaoqian; Jing, Xiaohuan; Hu, Tongyuan; Zhou, Jianwei; Chen, Jianwei; Xiao, Liang; Wang, Bo; Chen, Zhuang; Liu, Jing; Hu, Yiyin; Liu, Guilin; Liu, Shanshan; Liu, Junnian; Wei, Wenkang; Zou, Yuanqiang.
In: Microbiology Spectrum, Vol. 10, No. 1, e02417-21, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A Bacterial Genome and Culture Collection of Gut Microbial in Weanling Piglet
AU - Dong, Bo
AU - Lin, Xiaoqian
AU - Jing, Xiaohuan
AU - Hu, Tongyuan
AU - Zhou, Jianwei
AU - Chen, Jianwei
AU - Xiao, Liang
AU - Wang, Bo
AU - Chen, Zhuang
AU - Liu, Jing
AU - Hu, Yiyin
AU - Liu, Guilin
AU - Liu, Shanshan
AU - Liu, Junnian
AU - Wei, Wenkang
AU - Zou, Yuanqiang
N1 - Publisher Copyright: © 2022 Dong et al.
PY - 2022
Y1 - 2022
N2 - The microbiota hosted in the pig gastrointestinal tract are important to health of this biomedical model. However, the individual species and functional repertoires that make up the pig gut microbiome remain largely undefined. Here we comprehensively investigated the genomes and functions of the piglet gut microbiome using culture-based and metagenomics approaches. A collection included 266 cultured genomes and 482 metagenome- assembled genomes (MAGs) that were clustered to 428 species across 10 phyla was established. Among these clustered species, 333 genomes represent potential new species. Less matches between cultured genomes and MAGs revealed a substantial bias for the acquisition of reference genomes by the two strategies. Glycoside hydrolases was the dominant category of carbohydrate-active enzymes. Four-hundred forty-five secondary metabolite biosynthetic genes were predicted from 292 genomes with bacteriocin being the most. Pan genome analysis of Limosilactobacillus reuteri uncover the biosynthesis of reuterin was strain-specific and the production was experimentally determined. This study provides a comprehensive view of the microbiome composition and the function landscape of the gut of weanling piglets and a valuable bacterial resource for further experimentations.
AB - The microbiota hosted in the pig gastrointestinal tract are important to health of this biomedical model. However, the individual species and functional repertoires that make up the pig gut microbiome remain largely undefined. Here we comprehensively investigated the genomes and functions of the piglet gut microbiome using culture-based and metagenomics approaches. A collection included 266 cultured genomes and 482 metagenome- assembled genomes (MAGs) that were clustered to 428 species across 10 phyla was established. Among these clustered species, 333 genomes represent potential new species. Less matches between cultured genomes and MAGs revealed a substantial bias for the acquisition of reference genomes by the two strategies. Glycoside hydrolases was the dominant category of carbohydrate-active enzymes. Four-hundred forty-five secondary metabolite biosynthetic genes were predicted from 292 genomes with bacteriocin being the most. Pan genome analysis of Limosilactobacillus reuteri uncover the biosynthesis of reuterin was strain-specific and the production was experimentally determined. This study provides a comprehensive view of the microbiome composition and the function landscape of the gut of weanling piglets and a valuable bacterial resource for further experimentations.
KW - Functional repertoires
KW - Genome collection
KW - Limosilactobacillus reuteri
KW - Metagenome-assembled genomes
KW - Weanling piglet
U2 - 10.1128/spectrum.02417-21
DO - 10.1128/spectrum.02417-21
M3 - Journal article
C2 - 35171009
AN - SCOPUS:85125211824
VL - 10
JO - Microbiology spectrum
JF - Microbiology spectrum
SN - 2165-0497
IS - 1
M1 - e02417-21
ER -
ID: 302449576