A cell shrinkage-induced non-selective cation conductance with a novel pharmacology in Ehrlich-Lettre-ascites tumour cells.
Research output: Contribution to journal › Journal article › Research › peer-review
In whole-cell recordings on Ehrlich-Lettre-ascites tumour (ELA) cells, the shrinkage-induced activation of a cation conductance with a selectivity ratio P(Na):P(Li):P(K):P(choline):P(NMDG) of 1.00:0.97:0.88:0.03:0.01 was observed. In order of potency, this conductance was blocked by Gd(3+)=benzamil>amiloride>ethyl-isopropyl-amiloride (EIPA). In patch-clamp studies using the cell-attached configuration, a 14 pS channel became detectable that was reversibly activated upon hypertonic cell shrinkage. It is concluded that ELA cells express a shrinkage-induced cation channel that may reflect a molecular link between amiloride-sensitive and -insensitive channels. In addition, because of its pharmacological profile, it may possibly be related to epithelial Na+ channels (ENaCs).
Original language | English |
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Journal | FEBS Letters |
Volume | 539 |
Issue number | 1-3 |
Pages (from-to) | 115-9 |
Number of pages | 4 |
ISSN | 0014-5793 |
DOIs | |
Publication status | Published - 2003 |
Bibliographical note
Keywords: Animals; Carcinoma, Ehrlich Tumor; Cations; Cell Size; Electric Conductivity; Ion Channels; Patch-Clamp Techniques; Tumor Cells, Cultured
ID: 6768745