A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli. / Gonzalez de Valdivia, Ernesto I; Isaksson, Leif A.

In: Nucleic Acids Symposium Series, Vol. 32, No. 17, 01.01.2004, p. 5198-205.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Gonzalez de Valdivia, EI & Isaksson, LA 2004, 'A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli', Nucleic Acids Symposium Series, vol. 32, no. 17, pp. 5198-205. https://doi.org/10.1093/nar/gkh857

APA

Gonzalez de Valdivia, E. I., & Isaksson, L. A. (2004). A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli. Nucleic Acids Symposium Series, 32(17), 5198-205. https://doi.org/10.1093/nar/gkh857

Vancouver

Gonzalez de Valdivia EI, Isaksson LA. A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli. Nucleic Acids Symposium Series. 2004 Jan 1;32(17):5198-205. https://doi.org/10.1093/nar/gkh857

Author

Gonzalez de Valdivia, Ernesto I ; Isaksson, Leif A. / A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli. In: Nucleic Acids Symposium Series. 2004 ; Vol. 32, No. 17. pp. 5198-205.

Bibtex

@article{9269706515994a8ab7d07529f3f2c5fb,
title = "A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli",
abstract = "The influences on gene expression by codons at positions +2, +3, +5 and +7 downstream of the initiation codon have been compared. Most of the +2 codons that are known to give low gene expression are associated with a higher expression if placed at the later positions. The NGG codons AGG, CGG, UGG and GGG, but not GGN or GNG (where N is non-G), are unique since they are associated with a very low gene expression also if located at positions +2, +3 and +5. All codons, including NGG, give a normal gene expression if placed at positions +7. The negative effect by the NGG codons is true for both the lacZ and 3A' model genes. The low expression is suggested to originate at the translational level, although it is not the result of mRNA secondary structure or a lowered intracellular mRNA pool.",
keywords = "Codon, Codon, Initiator, Escherichia coli, Gene Expression Regulation, Bacterial, Protein Biosynthesis, RNA, Messenger, Transcription, Genetic",
author = "{Gonzalez de Valdivia}, {Ernesto I} and Isaksson, {Leif A}",
year = "2004",
month = jan,
day = "1",
doi = "10.1093/nar/gkh857",
language = "English",
volume = "32",
pages = "5198--205",
journal = "Nucleic acids symposium series",
issn = "0261-3166",
publisher = "Oxford University Press",
number = "17",

}

RIS

TY - JOUR

T1 - A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli

AU - Gonzalez de Valdivia, Ernesto I

AU - Isaksson, Leif A

PY - 2004/1/1

Y1 - 2004/1/1

N2 - The influences on gene expression by codons at positions +2, +3, +5 and +7 downstream of the initiation codon have been compared. Most of the +2 codons that are known to give low gene expression are associated with a higher expression if placed at the later positions. The NGG codons AGG, CGG, UGG and GGG, but not GGN or GNG (where N is non-G), are unique since they are associated with a very low gene expression also if located at positions +2, +3 and +5. All codons, including NGG, give a normal gene expression if placed at positions +7. The negative effect by the NGG codons is true for both the lacZ and 3A' model genes. The low expression is suggested to originate at the translational level, although it is not the result of mRNA secondary structure or a lowered intracellular mRNA pool.

AB - The influences on gene expression by codons at positions +2, +3, +5 and +7 downstream of the initiation codon have been compared. Most of the +2 codons that are known to give low gene expression are associated with a higher expression if placed at the later positions. The NGG codons AGG, CGG, UGG and GGG, but not GGN or GNG (where N is non-G), are unique since they are associated with a very low gene expression also if located at positions +2, +3 and +5. All codons, including NGG, give a normal gene expression if placed at positions +7. The negative effect by the NGG codons is true for both the lacZ and 3A' model genes. The low expression is suggested to originate at the translational level, although it is not the result of mRNA secondary structure or a lowered intracellular mRNA pool.

KW - Codon

KW - Codon, Initiator

KW - Escherichia coli

KW - Gene Expression Regulation, Bacterial

KW - Protein Biosynthesis

KW - RNA, Messenger

KW - Transcription, Genetic

U2 - 10.1093/nar/gkh857

DO - 10.1093/nar/gkh857

M3 - Journal article

C2 - 15459289

VL - 32

SP - 5198

EP - 5205

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

SN - 0261-3166

IS - 17

ER -

ID: 32642081