A fibroblast-associated antigen: Characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells

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Standard

A fibroblast-associated antigen: Characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells. / Rønnov-Jessen, Lone; Celis, Julio E.; van Deurs, Bo; Petersen, Ole William.

In: Journal of Histochemistry and Cytochemistry, Vol. 40, No. 4, 1992, p. 475-486.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rønnov-Jessen, L, Celis, JE, van Deurs, B & Petersen, OW 1992, 'A fibroblast-associated antigen: Characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells', Journal of Histochemistry and Cytochemistry, vol. 40, no. 4, pp. 475-486. <http://www.jhc.org/cgi/content/abstract/40/4/475>

APA

Rønnov-Jessen, L., Celis, J. E., van Deurs, B., & Petersen, O. W. (1992). A fibroblast-associated antigen: Characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells. Journal of Histochemistry and Cytochemistry, 40(4), 475-486. http://www.jhc.org/cgi/content/abstract/40/4/475

Vancouver

Rønnov-Jessen L, Celis JE, van Deurs B, Petersen OW. A fibroblast-associated antigen: Characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells. Journal of Histochemistry and Cytochemistry. 1992;40(4):475-486.

Author

Rønnov-Jessen, Lone ; Celis, Julio E. ; van Deurs, Bo ; Petersen, Ole William. / A fibroblast-associated antigen: Characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells. In: Journal of Histochemistry and Cytochemistry. 1992 ; Vol. 40, No. 4. pp. 475-486.

Bibtex

@article{eea68820c53611dd8ca2000ea68e967b,
title = "A fibroblast-associated antigen: Characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells",
abstract = "Fibroblasts with smooth muscle differentiation are frequently derived from human breast tissue. Immunofluorescence cytochemistry of a fibroblast-associated antigen recognized by a monoclonal antibody (MAb), 1B10, was analyzed with a view to discriminating smooth muscle differentiated fibroblasts from vascular smooth muscle cells. The antigen was detected on the cell surface and in cathepsin D-positive and acridine orange-accumulating vesicular compartments of fibroblasts. Ultrastructurally, the antigen was revealed in coated pits and in endosomal and lysosomal structures. 1B10 recognized three major brands migrating at apparent Mr of 38,000, 45,000, and 80,000, in addition to many minor bands between Mr 45,000 and 97,000, including Mr 52,000. The Mr 45,000 and 38,000 were associated with the cell membrane and Mr 52,000 as well as Mr 38,000 were associated with the lysosomes. The 1B10 immunoreactivity was specific to fibroblasts and smooth muscle differentiated fibroblasts within the context of vascular smooth muscle cells.",
author = "Lone R{\o}nnov-Jessen and Celis, {Julio E.} and {van Deurs}, Bo and Petersen, {Ole William}",
year = "1992",
language = "English",
volume = "40",
pages = "475--486",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "SAGE Publications",
number = "4",

}

RIS

TY - JOUR

T1 - A fibroblast-associated antigen: Characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells

AU - Rønnov-Jessen, Lone

AU - Celis, Julio E.

AU - van Deurs, Bo

AU - Petersen, Ole William

PY - 1992

Y1 - 1992

N2 - Fibroblasts with smooth muscle differentiation are frequently derived from human breast tissue. Immunofluorescence cytochemistry of a fibroblast-associated antigen recognized by a monoclonal antibody (MAb), 1B10, was analyzed with a view to discriminating smooth muscle differentiated fibroblasts from vascular smooth muscle cells. The antigen was detected on the cell surface and in cathepsin D-positive and acridine orange-accumulating vesicular compartments of fibroblasts. Ultrastructurally, the antigen was revealed in coated pits and in endosomal and lysosomal structures. 1B10 recognized three major brands migrating at apparent Mr of 38,000, 45,000, and 80,000, in addition to many minor bands between Mr 45,000 and 97,000, including Mr 52,000. The Mr 45,000 and 38,000 were associated with the cell membrane and Mr 52,000 as well as Mr 38,000 were associated with the lysosomes. The 1B10 immunoreactivity was specific to fibroblasts and smooth muscle differentiated fibroblasts within the context of vascular smooth muscle cells.

AB - Fibroblasts with smooth muscle differentiation are frequently derived from human breast tissue. Immunofluorescence cytochemistry of a fibroblast-associated antigen recognized by a monoclonal antibody (MAb), 1B10, was analyzed with a view to discriminating smooth muscle differentiated fibroblasts from vascular smooth muscle cells. The antigen was detected on the cell surface and in cathepsin D-positive and acridine orange-accumulating vesicular compartments of fibroblasts. Ultrastructurally, the antigen was revealed in coated pits and in endosomal and lysosomal structures. 1B10 recognized three major brands migrating at apparent Mr of 38,000, 45,000, and 80,000, in addition to many minor bands between Mr 45,000 and 97,000, including Mr 52,000. The Mr 45,000 and 38,000 were associated with the cell membrane and Mr 52,000 as well as Mr 38,000 were associated with the lysosomes. The 1B10 immunoreactivity was specific to fibroblasts and smooth muscle differentiated fibroblasts within the context of vascular smooth muscle cells.

M3 - Journal article

VL - 40

SP - 475

EP - 486

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 4

ER -

ID: 8947094