A Flp-nick system to study repair of a single protein-bound nick in vivo

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A Flp-nick system to study repair of a single protein-bound nick in vivo. / Nielsen, Ida; Bentsen, Iben Bach; Lisby, Michael; Hansen, Sabine; Mundbjerg, Kamilla; Andersen, Anni H; Bjergbaek, Lotte.

In: Nature Methods, Vol. 6, No. 10, 2009, p. 753-757.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nielsen, I, Bentsen, IB, Lisby, M, Hansen, S, Mundbjerg, K, Andersen, AH & Bjergbaek, L 2009, 'A Flp-nick system to study repair of a single protein-bound nick in vivo', Nature Methods, vol. 6, no. 10, pp. 753-757. https://doi.org/10.1038/nmeth.1372

APA

Nielsen, I., Bentsen, I. B., Lisby, M., Hansen, S., Mundbjerg, K., Andersen, A. H., & Bjergbaek, L. (2009). A Flp-nick system to study repair of a single protein-bound nick in vivo. Nature Methods, 6(10), 753-757. https://doi.org/10.1038/nmeth.1372

Vancouver

Nielsen I, Bentsen IB, Lisby M, Hansen S, Mundbjerg K, Andersen AH et al. A Flp-nick system to study repair of a single protein-bound nick in vivo. Nature Methods. 2009;6(10):753-757. https://doi.org/10.1038/nmeth.1372

Author

Nielsen, Ida ; Bentsen, Iben Bach ; Lisby, Michael ; Hansen, Sabine ; Mundbjerg, Kamilla ; Andersen, Anni H ; Bjergbaek, Lotte. / A Flp-nick system to study repair of a single protein-bound nick in vivo. In: Nature Methods. 2009 ; Vol. 6, No. 10. pp. 753-757.

Bibtex

@article{422a49a0f3a311deba73000ea68e967b,
title = "A Flp-nick system to study repair of a single protein-bound nick in vivo",
abstract = "We present the Flp-nick system, which allows introduction of a protein-bound nick at a single genomic site in Saccharomyces cerevisiae and thus mimics a stabilized topoisomerase I-DNA cleavage complex. We took advantage of a mutant Flp recombinase that can introduce a nick at a specific Flp recombinase recognition target site that has been integrated in the yeast genome. The genetic requirement for cells to cope with this insult is the same as for cells treated with camptothecin, which traps topoisomerase I-DNA cleavage complexes genome-wide. Hence, a single protein-bound nick is enough to kill cells if functional repair pathways are lacking. The Flp-nick system can be used to dissect repair, checkpoint and replication fork management pathways activated by a single genomic insult, and it allows the study of events at the damage site, which so far has been impossible to address.",
author = "Ida Nielsen and Bentsen, {Iben Bach} and Michael Lisby and Sabine Hansen and Kamilla Mundbjerg and Andersen, {Anni H} and Lotte Bjergbaek",
note = "Keywords: DNA Breaks, Single-Stranded; DNA Damage; DNA Nucleotidyltransferases; DNA Repair; DNA, Bacterial; Mutagenesis, Site-Directed; Saccharomyces cerevisiae",
year = "2009",
doi = "10.1038/nmeth.1372",
language = "English",
volume = "6",
pages = "753--757",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "nature publishing group",
number = "10",

}

RIS

TY - JOUR

T1 - A Flp-nick system to study repair of a single protein-bound nick in vivo

AU - Nielsen, Ida

AU - Bentsen, Iben Bach

AU - Lisby, Michael

AU - Hansen, Sabine

AU - Mundbjerg, Kamilla

AU - Andersen, Anni H

AU - Bjergbaek, Lotte

N1 - Keywords: DNA Breaks, Single-Stranded; DNA Damage; DNA Nucleotidyltransferases; DNA Repair; DNA, Bacterial; Mutagenesis, Site-Directed; Saccharomyces cerevisiae

PY - 2009

Y1 - 2009

N2 - We present the Flp-nick system, which allows introduction of a protein-bound nick at a single genomic site in Saccharomyces cerevisiae and thus mimics a stabilized topoisomerase I-DNA cleavage complex. We took advantage of a mutant Flp recombinase that can introduce a nick at a specific Flp recombinase recognition target site that has been integrated in the yeast genome. The genetic requirement for cells to cope with this insult is the same as for cells treated with camptothecin, which traps topoisomerase I-DNA cleavage complexes genome-wide. Hence, a single protein-bound nick is enough to kill cells if functional repair pathways are lacking. The Flp-nick system can be used to dissect repair, checkpoint and replication fork management pathways activated by a single genomic insult, and it allows the study of events at the damage site, which so far has been impossible to address.

AB - We present the Flp-nick system, which allows introduction of a protein-bound nick at a single genomic site in Saccharomyces cerevisiae and thus mimics a stabilized topoisomerase I-DNA cleavage complex. We took advantage of a mutant Flp recombinase that can introduce a nick at a specific Flp recombinase recognition target site that has been integrated in the yeast genome. The genetic requirement for cells to cope with this insult is the same as for cells treated with camptothecin, which traps topoisomerase I-DNA cleavage complexes genome-wide. Hence, a single protein-bound nick is enough to kill cells if functional repair pathways are lacking. The Flp-nick system can be used to dissect repair, checkpoint and replication fork management pathways activated by a single genomic insult, and it allows the study of events at the damage site, which so far has been impossible to address.

U2 - 10.1038/nmeth.1372

DO - 10.1038/nmeth.1372

M3 - Journal article

C2 - 19749762

VL - 6

SP - 753

EP - 757

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 10

ER -

ID: 16586255