A H2S microsensor for profiling biofilms and sediments: Application in an acidic lake sediment
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A H2S microsensor for profiling biofilms and sediments : Application in an acidic lake sediment. / Kühl, Michael; Steuckart, Carsten; Eickert, Gabriele; Jeroschewski, Paul.
In: Aquatic Microbial Ecology, Vol. 15, No. 2, 1998, p. 201-209.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A H2S microsensor for profiling biofilms and sediments
T2 - Application in an acidic lake sediment
AU - Kühl, Michael
AU - Steuckart, Carsten
AU - Eickert, Gabriele
AU - Jeroschewski, Paul
PY - 1998
Y1 - 1998
N2 - We developed a microsensor for the amperometric detection of dissolved hydrogen sulfide, H2S, in sediments and biofilms. The microsensor exhibits a fast (t90 <0.2 to 0.5 s) and linear response to H2S over a concentration range of 1 to >1000 μmol H2S l-1, and has a low stirring dependency of the microsensor signal (<1 to 2%). We used the new microsensor to obtain the first microprofiles of H2S in an acidic lake sediment with a several cm thick flocculant surface layer. Despite the low pH of 4.6, a relative low SO4 2- level in the lake water, and a broad O2 respiration zone of ca 6 mm, we were able to measure H2S depth profiles in the sediment at a good resolution, that allowed for calculation of specific sulfate reduction and H2S oxidation activities. Such calculations showed highest sulfate reduction activity in the anoxic sediment down to ca 20 mm depth. A comparison of calculated areal rates of O2 consumption and sulfate reduction indicated that sulfate reduction accounted for up to 13% of total organic carbon mineralization in the acidic sediment. All produced H2S was reoxidizecl aerobically with O2 at the oxic-anoxic interface. In addition to its good performance in acidic environments, the new H2S microsensor has proven useful for sulfide measurements in neutral and moderate alkaline (pH < 9) biofilms and sediments, and thus is a true alternative to the traditionally used potentiometric Ag/Ag2S microelectrode for most applications in aquatic ecology and biogeochemistry.
AB - We developed a microsensor for the amperometric detection of dissolved hydrogen sulfide, H2S, in sediments and biofilms. The microsensor exhibits a fast (t90 <0.2 to 0.5 s) and linear response to H2S over a concentration range of 1 to >1000 μmol H2S l-1, and has a low stirring dependency of the microsensor signal (<1 to 2%). We used the new microsensor to obtain the first microprofiles of H2S in an acidic lake sediment with a several cm thick flocculant surface layer. Despite the low pH of 4.6, a relative low SO4 2- level in the lake water, and a broad O2 respiration zone of ca 6 mm, we were able to measure H2S depth profiles in the sediment at a good resolution, that allowed for calculation of specific sulfate reduction and H2S oxidation activities. Such calculations showed highest sulfate reduction activity in the anoxic sediment down to ca 20 mm depth. A comparison of calculated areal rates of O2 consumption and sulfate reduction indicated that sulfate reduction accounted for up to 13% of total organic carbon mineralization in the acidic sediment. All produced H2S was reoxidizecl aerobically with O2 at the oxic-anoxic interface. In addition to its good performance in acidic environments, the new H2S microsensor has proven useful for sulfide measurements in neutral and moderate alkaline (pH < 9) biofilms and sediments, and thus is a true alternative to the traditionally used potentiometric Ag/Ag2S microelectrode for most applications in aquatic ecology and biogeochemistry.
KW - Acidic sediment
KW - Freshwater
KW - Hydrogen sulfide
KW - Microsensor
KW - Sulfate reduction
KW - Sulfide oxidation
U2 - 10.3354/ame015201
DO - 10.3354/ame015201
M3 - Journal article
AN - SCOPUS:1542605767
VL - 15
SP - 201
EP - 209
JO - Aquatic Microbial Ecology
JF - Aquatic Microbial Ecology
SN - 0948-3055
IS - 2
ER -
ID: 201682829