A "new" supertypic HLA-DP related determinant detected by primed lymphocyte typing (PLT).
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A "new" supertypic HLA-DP related determinant detected by primed lymphocyte typing (PLT). / Ødum, Niels; Hofmann, B; Hyldig-Nielsen, J J; Jakobsen, B K; Morling, N; Platz, P; Ryder, Lars P.; Svejgaard, Arne.
In: HLA, Vol. 29, No. 2, 1987, p. 101-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A "new" supertypic HLA-DP related determinant detected by primed lymphocyte typing (PLT).
AU - Ødum, Niels
AU - Hofmann, B
AU - Hyldig-Nielsen, J J
AU - Jakobsen, B K
AU - Morling, N
AU - Platz, P
AU - Ryder, Lars P.
AU - Svejgaard, Arne
N1 - Keywords: Antibodies, Monoclonal; Epitopes; HLA-D Antigens; HLA-DP Antigens; HLA-DR Antigens; Humans; Lymphocytes
PY - 1987
Y1 - 1987
N2 - Primed Lymphocyte Typing (PLT) with local (CDP) and the 9th International Histocompatibility Workshop reagents (GNN) revealed "cross-reactions" between HLA-DPw6 and the GNN2B but not other DPw2 PLT-cells (GNN2A, CDP2A, CDP2B). We raised and bulk-expanded a well discriminating PLT-reagent (the JET-reagent) from an HLA-DR identical, DP, GNN2A, CDP2A, CDP2B compatible and GNN2B incompatible responder/stimulator combination. The JET-reagent defined a "new" determinant, JET, which was present in 11% of Danes. The JET determinant was associated with DPw2, w6, and DP-blank: 66% of DPw6 and DP-blank and 20% of DPw2-positive individuals were JET-positive. All JET-positive individuals belonged to this group, and six of these had two DP-antigens. JET segregated with DP-blank in three and with DPw6 in two informative families. In an HLA-DR/DPw6 recombinant family, JET segregated with DPw6 in the recombinant haplotype. The JET PLT-responses against all of six different JET-positive stimulators including the specific stimulator were strongly inhibited by the monoclonal antibody (MoAb) Tü39, which preferentially reacts with DP-molecules, but not by other HLA-specific MoAbs. The results indicate that JET is either a "new" supertypic DP-related specificity or a determinant, which shares epitopes with DP, of a "new" locus and in linkage disequilibrium with DP.
AB - Primed Lymphocyte Typing (PLT) with local (CDP) and the 9th International Histocompatibility Workshop reagents (GNN) revealed "cross-reactions" between HLA-DPw6 and the GNN2B but not other DPw2 PLT-cells (GNN2A, CDP2A, CDP2B). We raised and bulk-expanded a well discriminating PLT-reagent (the JET-reagent) from an HLA-DR identical, DP, GNN2A, CDP2A, CDP2B compatible and GNN2B incompatible responder/stimulator combination. The JET-reagent defined a "new" determinant, JET, which was present in 11% of Danes. The JET determinant was associated with DPw2, w6, and DP-blank: 66% of DPw6 and DP-blank and 20% of DPw2-positive individuals were JET-positive. All JET-positive individuals belonged to this group, and six of these had two DP-antigens. JET segregated with DP-blank in three and with DPw6 in two informative families. In an HLA-DR/DPw6 recombinant family, JET segregated with DPw6 in the recombinant haplotype. The JET PLT-responses against all of six different JET-positive stimulators including the specific stimulator were strongly inhibited by the monoclonal antibody (MoAb) Tü39, which preferentially reacts with DP-molecules, but not by other HLA-specific MoAbs. The results indicate that JET is either a "new" supertypic DP-related specificity or a determinant, which shares epitopes with DP, of a "new" locus and in linkage disequilibrium with DP.
M3 - Journal article
C2 - 2440144
VL - 29
SP - 101
EP - 109
JO - HLA
JF - HLA
SN - 2059-2302
IS - 2
ER -
ID: 10638148