A Novel Fluorescence-Based Screen for Inhibitors of the Initiation of DNA Replication in Bacteria

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A Novel Fluorescence-Based Screen for Inhibitors of the Initiation of DNA Replication in Bacteria. / Klitgaard, Rasmus N.; Løbner-Olesen, Anders.

In: Current Drug Discovery Technologies, Vol. 16, No. 3, 2019, p. 272-277.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Klitgaard, RN & Løbner-Olesen, A 2019, 'A Novel Fluorescence-Based Screen for Inhibitors of the Initiation of DNA Replication in Bacteria', Current Drug Discovery Technologies, vol. 16, no. 3, pp. 272-277. https://doi.org/10.2174/1570163815666180423115514

APA

Klitgaard, R. N., & Løbner-Olesen, A. (2019). A Novel Fluorescence-Based Screen for Inhibitors of the Initiation of DNA Replication in Bacteria. Current Drug Discovery Technologies, 16(3), 272-277. https://doi.org/10.2174/1570163815666180423115514

Vancouver

Klitgaard RN, Løbner-Olesen A. A Novel Fluorescence-Based Screen for Inhibitors of the Initiation of DNA Replication in Bacteria. Current Drug Discovery Technologies. 2019;16(3):272-277. https://doi.org/10.2174/1570163815666180423115514

Author

Klitgaard, Rasmus N. ; Løbner-Olesen, Anders. / A Novel Fluorescence-Based Screen for Inhibitors of the Initiation of DNA Replication in Bacteria. In: Current Drug Discovery Technologies. 2019 ; Vol. 16, No. 3. pp. 272-277.

Bibtex

@article{b8177f2fd6354178baa5f7b6cc1aa3b2,
title = "A Novel Fluorescence-Based Screen for Inhibitors of the Initiation of DNA Replication in Bacteria",
abstract = "BACKGROUND: One of many strategies to overcome antibiotic resistance is the discovery of compounds targeting cellular processes, which have not yet been exploited.METHODS AND MATERIALS: Using various genetic tools, we constructed a novel high throughput, cell based, fluorescence screen for inhibitors of chromosome replication initiation in bacteria.RESULTS: The screen was validated by expression of an intra-cellular cyclic peptide interfering with the initiator protein DnaA and by over-expression of the negative initiation regulator SeqA. We also demonstrated that neither tetracycline nor ciprofloxacin triggers a false positive result. Finally, 400 extracts isolated mainly from filamentous actinomycetes were subjected to the screen.CONCLUSION: We concluded that the presented screen is applicable for identifying putative inhibitors of DNA replication initiation in a high throughput setup.",
author = "Klitgaard, {Rasmus N.} and Anders L{\o}bner-Olesen",
note = "Copyright{\textcopyright} Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.",
year = "2019",
doi = "10.2174/1570163815666180423115514",
language = "English",
volume = "16",
pages = "272--277",
journal = "Current Drug Discovery Technologies",
issn = "1570-1638",
publisher = "Bentham Science Publishers",
number = "3",

}

RIS

TY - JOUR

T1 - A Novel Fluorescence-Based Screen for Inhibitors of the Initiation of DNA Replication in Bacteria

AU - Klitgaard, Rasmus N.

AU - Løbner-Olesen, Anders

N1 - Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

PY - 2019

Y1 - 2019

N2 - BACKGROUND: One of many strategies to overcome antibiotic resistance is the discovery of compounds targeting cellular processes, which have not yet been exploited.METHODS AND MATERIALS: Using various genetic tools, we constructed a novel high throughput, cell based, fluorescence screen for inhibitors of chromosome replication initiation in bacteria.RESULTS: The screen was validated by expression of an intra-cellular cyclic peptide interfering with the initiator protein DnaA and by over-expression of the negative initiation regulator SeqA. We also demonstrated that neither tetracycline nor ciprofloxacin triggers a false positive result. Finally, 400 extracts isolated mainly from filamentous actinomycetes were subjected to the screen.CONCLUSION: We concluded that the presented screen is applicable for identifying putative inhibitors of DNA replication initiation in a high throughput setup.

AB - BACKGROUND: One of many strategies to overcome antibiotic resistance is the discovery of compounds targeting cellular processes, which have not yet been exploited.METHODS AND MATERIALS: Using various genetic tools, we constructed a novel high throughput, cell based, fluorescence screen for inhibitors of chromosome replication initiation in bacteria.RESULTS: The screen was validated by expression of an intra-cellular cyclic peptide interfering with the initiator protein DnaA and by over-expression of the negative initiation regulator SeqA. We also demonstrated that neither tetracycline nor ciprofloxacin triggers a false positive result. Finally, 400 extracts isolated mainly from filamentous actinomycetes were subjected to the screen.CONCLUSION: We concluded that the presented screen is applicable for identifying putative inhibitors of DNA replication initiation in a high throughput setup.

U2 - 10.2174/1570163815666180423115514

DO - 10.2174/1570163815666180423115514

M3 - Journal article

C2 - 29683093

VL - 16

SP - 272

EP - 277

JO - Current Drug Discovery Technologies

JF - Current Drug Discovery Technologies

SN - 1570-1638

IS - 3

ER -

ID: 196438494