A spontaneous mutant of microcystin biosynthesis: genetic characterization and effect on Daphnia

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A spontaneous mutant of microcystin biosynthesis: genetic characterization and effect on Daphnia. / Kabernick, M.; Rohrlack, T.; Christoffersen, K.; Neilan, B.A.

In: Environmental Microbiology, Vol. 3, No. 11, 2001, p. 669-679.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kabernick, M, Rohrlack, T, Christoffersen, K & Neilan, BA 2001, 'A spontaneous mutant of microcystin biosynthesis: genetic characterization and effect on Daphnia', Environmental Microbiology, vol. 3, no. 11, pp. 669-679. https://doi.org/10.1046/j.1462-2920.2001.00241.x

APA

Kabernick, M., Rohrlack, T., Christoffersen, K., & Neilan, B. A. (2001). A spontaneous mutant of microcystin biosynthesis: genetic characterization and effect on Daphnia. Environmental Microbiology, 3(11), 669-679. https://doi.org/10.1046/j.1462-2920.2001.00241.x

Vancouver

Kabernick M, Rohrlack T, Christoffersen K, Neilan BA. A spontaneous mutant of microcystin biosynthesis: genetic characterization and effect on Daphnia. Environmental Microbiology. 2001;3(11):669-679. https://doi.org/10.1046/j.1462-2920.2001.00241.x

Author

Kabernick, M. ; Rohrlack, T. ; Christoffersen, K. ; Neilan, B.A. / A spontaneous mutant of microcystin biosynthesis: genetic characterization and effect on Daphnia. In: Environmental Microbiology. 2001 ; Vol. 3, No. 11. pp. 669-679.

Bibtex

@article{0f22b10074c611dbbee902004c4f4f50,
title = "A spontaneous mutant of microcystin biosynthesis: genetic characterization and effect on Daphnia",
abstract = "Microcystis aeruginosa strain MRC is unique in its' possession of the mcyA-J gene cluster, which encodes microcystin synthetase, but its' inability to produce microcystins. M. aeruginosa strain MRD is genetically identical to MRC at numerous genomic loci examined, but produces a variety of microcystins, mainly with the amino acid tyrosine in the molecule. Zooplankton studies with Daphnia galeata and D. pulicaria, using the mutant (MRC) and its' wild type (MRD), showed for the first time that microcystins other than microcystin-LR can be responsible for the poisoning of Daphnia by Microcystis. Regardless of microcystin content, both Daphnia exhibited significantly reduced ingestion rates when fed with either strain of M. aeruginosa compared with the green alga Scenedesmus acutus. A disruption of the molting process in both Daphnia spp. was noted when these species were fed with MRC cells. Such symptoms on Daphnia have not been previously reported for cyanobacteria and may point to a bioactive compound, other than microcystin, which inhibits the hardening of protein–chitin complexes in Daphnia.",
author = "M. Kabernick and T. Rohrlack and K. Christoffersen and B.A. Neilan",
year = "2001",
doi = "10.1046/j.1462-2920.2001.00241.x",
language = "English",
volume = "3",
pages = "669--679",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "Wiley-Blackwell",
number = "11",

}

RIS

TY - JOUR

T1 - A spontaneous mutant of microcystin biosynthesis: genetic characterization and effect on Daphnia

AU - Kabernick, M.

AU - Rohrlack, T.

AU - Christoffersen, K.

AU - Neilan, B.A.

PY - 2001

Y1 - 2001

N2 - Microcystis aeruginosa strain MRC is unique in its' possession of the mcyA-J gene cluster, which encodes microcystin synthetase, but its' inability to produce microcystins. M. aeruginosa strain MRD is genetically identical to MRC at numerous genomic loci examined, but produces a variety of microcystins, mainly with the amino acid tyrosine in the molecule. Zooplankton studies with Daphnia galeata and D. pulicaria, using the mutant (MRC) and its' wild type (MRD), showed for the first time that microcystins other than microcystin-LR can be responsible for the poisoning of Daphnia by Microcystis. Regardless of microcystin content, both Daphnia exhibited significantly reduced ingestion rates when fed with either strain of M. aeruginosa compared with the green alga Scenedesmus acutus. A disruption of the molting process in both Daphnia spp. was noted when these species were fed with MRC cells. Such symptoms on Daphnia have not been previously reported for cyanobacteria and may point to a bioactive compound, other than microcystin, which inhibits the hardening of protein–chitin complexes in Daphnia.

AB - Microcystis aeruginosa strain MRC is unique in its' possession of the mcyA-J gene cluster, which encodes microcystin synthetase, but its' inability to produce microcystins. M. aeruginosa strain MRD is genetically identical to MRC at numerous genomic loci examined, but produces a variety of microcystins, mainly with the amino acid tyrosine in the molecule. Zooplankton studies with Daphnia galeata and D. pulicaria, using the mutant (MRC) and its' wild type (MRD), showed for the first time that microcystins other than microcystin-LR can be responsible for the poisoning of Daphnia by Microcystis. Regardless of microcystin content, both Daphnia exhibited significantly reduced ingestion rates when fed with either strain of M. aeruginosa compared with the green alga Scenedesmus acutus. A disruption of the molting process in both Daphnia spp. was noted when these species were fed with MRC cells. Such symptoms on Daphnia have not been previously reported for cyanobacteria and may point to a bioactive compound, other than microcystin, which inhibits the hardening of protein–chitin complexes in Daphnia.

U2 - 10.1046/j.1462-2920.2001.00241.x

DO - 10.1046/j.1462-2920.2001.00241.x

M3 - Journal article

VL - 3

SP - 669

EP - 679

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 11

ER -

ID: 144647