A unifying model for extrachromosomal circular DNA load in eukaryotic cells

Research output: Contribution to journal › Review › Research › peer-review

Standard

A unifying model for extrachromosomal circular DNA load in eukaryotic cells. / Arrey, Gerard; Keating, Samuel T.; Regenberg, Birgitte.

In: Seminars in Cell and Developmental Biology, Vol. 128, 2022, p. 40-50.

Research output: Contribution to journal › Review › Research › peer-review

Harvard

Arrey, G, Keating, ST & Regenberg, B 2022, 'A unifying model for extrachromosomal circular DNA load in eukaryotic cells', Seminars in Cell and Developmental Biology, vol. 128, pp. 40-50. https://doi.org/10.1016/j.semcdb.2022.03.002

APA

Arrey, G., Keating, S. T., & Regenberg, B. (2022). A unifying model for extrachromosomal circular DNA load in eukaryotic cells. Seminars in Cell and Developmental Biology, 128, 40-50. https://doi.org/10.1016/j.semcdb.2022.03.002

Vancouver

Arrey G, Keating ST, Regenberg B. A unifying model for extrachromosomal circular DNA load in eukaryotic cells. Seminars in Cell and Developmental Biology. 2022;128:40-50. https://doi.org/10.1016/j.semcdb.2022.03.002

Author

Arrey, Gerard ; Keating, Samuel T. ; Regenberg, Birgitte. / A unifying model for extrachromosomal circular DNA load in eukaryotic cells. In: Seminars in Cell and Developmental Biology. 2022 ; Vol. 128. pp. 40-50.

Bibtex

@article{ceab972619544c22b65a5bbea4232fc8,
title = "A unifying model for extrachromosomal circular DNA load in eukaryotic cells",
abstract = "Extrachromosomal circular DNA (eccDNA) with exons and whole genes are common features of eukaryotic cells. Work from especially tumours and the yeast Saccharomyces cerevisiae has revealed that eccDNA can provide large selective advantages and disadvantages. Besides the phenotypic effect due to expression of an eccDNA fragment, eccDNA is different from other mutations in that it is released from 1:1 segregation during cell division. This means that eccDNA can quickly change copy number, pickup secondary mutations and reintegrate into a chromosome to establish substantial genetic variation that could not have evolved via canonical mechanisms. We propose a unifying 5-factor model for conceptualizing the eccDNA load of a eukaryotic cell, emphasizing formation, replication, segregation, selection and elimination. We suggest that the magnitude of these sequential events and their interactions determine the copy number of eccDNA in mitotically dividing cells. We believe that our model will provide a coherent framework for eccDNA research, to understand its biology and the factors that can be manipulated to modulate eccDNA load in eukaryotic cells.",
keywords = "Cancer, DM, EcDNA, Evolution, Synthetic biology",
author = "Gerard Arrey and Keating, {Samuel T.} and Birgitte Regenberg",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
doi = "10.1016/j.semcdb.2022.03.002",
language = "English",
volume = "128",
pages = "40--50",
journal = "Seminars in Cell and Developmental Biology",
issn = "1084-9521",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - A unifying model for extrachromosomal circular DNA load in eukaryotic cells

AU - Arrey, Gerard

AU - Keating, Samuel T.

AU - Regenberg, Birgitte

N1 - Publisher Copyright: © 2022 The Authors

PY - 2022

Y1 - 2022

N2 - Extrachromosomal circular DNA (eccDNA) with exons and whole genes are common features of eukaryotic cells. Work from especially tumours and the yeast Saccharomyces cerevisiae has revealed that eccDNA can provide large selective advantages and disadvantages. Besides the phenotypic effect due to expression of an eccDNA fragment, eccDNA is different from other mutations in that it is released from 1:1 segregation during cell division. This means that eccDNA can quickly change copy number, pickup secondary mutations and reintegrate into a chromosome to establish substantial genetic variation that could not have evolved via canonical mechanisms. We propose a unifying 5-factor model for conceptualizing the eccDNA load of a eukaryotic cell, emphasizing formation, replication, segregation, selection and elimination. We suggest that the magnitude of these sequential events and their interactions determine the copy number of eccDNA in mitotically dividing cells. We believe that our model will provide a coherent framework for eccDNA research, to understand its biology and the factors that can be manipulated to modulate eccDNA load in eukaryotic cells.

AB - Extrachromosomal circular DNA (eccDNA) with exons and whole genes are common features of eukaryotic cells. Work from especially tumours and the yeast Saccharomyces cerevisiae has revealed that eccDNA can provide large selective advantages and disadvantages. Besides the phenotypic effect due to expression of an eccDNA fragment, eccDNA is different from other mutations in that it is released from 1:1 segregation during cell division. This means that eccDNA can quickly change copy number, pickup secondary mutations and reintegrate into a chromosome to establish substantial genetic variation that could not have evolved via canonical mechanisms. We propose a unifying 5-factor model for conceptualizing the eccDNA load of a eukaryotic cell, emphasizing formation, replication, segregation, selection and elimination. We suggest that the magnitude of these sequential events and their interactions determine the copy number of eccDNA in mitotically dividing cells. We believe that our model will provide a coherent framework for eccDNA research, to understand its biology and the factors that can be manipulated to modulate eccDNA load in eukaryotic cells.

KW - Cancer

KW - DM

KW - EcDNA

KW - Evolution

KW - Synthetic biology

U2 - 10.1016/j.semcdb.2022.03.002

DO - 10.1016/j.semcdb.2022.03.002

M3 - Review

C2 - 35292190

AN - SCOPUS:85126306722

VL - 128

SP - 40

EP - 50

JO - Seminars in Cell and Developmental Biology

JF - Seminars in Cell and Developmental Biology

SN - 1084-9521

ER -

ID: 315856873