Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3
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Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3. / Kjaerulff, S; Davey, William John; Nielsen, O; Nielsen, Olaf.
In: Molecular and Cellular Biology, Vol. 14, No. 6, 01.06.1994, p. 3895-905.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3
AU - Kjaerulff, S
AU - Davey, William John
AU - Nielsen, O
AU - Nielsen, Olaf
PY - 1994/6/1
Y1 - 1994/6/1
N2 - We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.
AB - We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.
KW - Amino Acid Sequence
KW - Base Sequence
KW - Cloning, Molecular
KW - Conserved Sequence
KW - DNA Primers
KW - Genes, Fungal
KW - Molecular Sequence Data
KW - Mutagenesis
KW - Open Reading Frames
KW - Peptide Biosynthesis
KW - Peptides
KW - Pheromones
KW - Polymerase Chain Reaction
KW - Restriction Mapping
KW - Schizosaccharomyces
KW - Sequence Deletion
KW - Sequence Homology, Amino Acid
KW - Sequence Homology, Nucleic Acid
KW - Transcription, Genetic
M3 - Journal article
C2 - 8196631
VL - 14
SP - 3895
EP - 3905
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 6
ER -
ID: 33577404