Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus
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Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus. / Qiu, Jinlong; Fiil, Berthe Katrine; Petersen, Klaus; Nielsen, Henrik Bjørn; Botanga, Christopher J; Thorgrimsen, Stephan Pascal; Palma, Kristoffer; Suarez Rodriguez, Maria Cristina; Sandbech-Clausen, Signe; Lichota, Jacek; Brodersen, Peter; Grasser, Klaus D; Mattsson, Ole; Glazebrook, Jane; Mundy, John; Petersen, Morten.
In: EMBO Journal, Vol. 27, No. 16, 2008, p. 2214-2221.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus
AU - Qiu, Jinlong
AU - Fiil, Berthe Katrine
AU - Petersen, Klaus
AU - Nielsen, Henrik Bjørn
AU - Botanga, Christopher J
AU - Thorgrimsen, Stephan Pascal
AU - Palma, Kristoffer
AU - Suarez Rodriguez, Maria Cristina
AU - Sandbech-Clausen, Signe
AU - Lichota, Jacek
AU - Brodersen, Peter
AU - Grasser, Klaus D
AU - Mattsson, Ole
AU - Glazebrook, Jane
AU - Mundy, John
AU - Petersen, Morten
N1 - Keywords:Arabidopsis, defence response, MAP kinase, target genes, transcription factor
PY - 2008
Y1 - 2008
N2 - Plant and animal perception of microbes through pathogen surveillance proteins leads to MAP kinase signalling and the expression of defence genes. However, little is known about how plant MAP kinases regulate specific gene expression. We report that, in the absence of pathogens, Arabidopsis MAP kinase 4 (MPK4) exists in nuclear complexes with the WRKY33 transcription factor. This complex depends on the MPK4 substrate MKS1. Challenge with Pseudomonas syringae or flagellin leads to the activation of MPK4 and phosphorylation of MKS1. Subsequently, complexes with MKS1 and WRKY33 are released from MPK4, and WRKY33 targets the promoter of PHYTOALEXIN DEFICIENT3 (PAD3) encoding an enzyme required for the synthesis of antimicrobial camalexin. Hence, wrky33 mutants are impaired in the accumulation of PAD3 mRNA and camalexin production upon infection. That WRKY33 is an effector of MPK4 is further supported by the suppression of PAD3 expression in mpk4-wrky33 double mutant backgrounds. Our data establish direct links between MPK4 and innate immunity and provide an example of how a plant MAP kinase can regulate gene expression by releasing transcription factors in the nucleus upon activation.
AB - Plant and animal perception of microbes through pathogen surveillance proteins leads to MAP kinase signalling and the expression of defence genes. However, little is known about how plant MAP kinases regulate specific gene expression. We report that, in the absence of pathogens, Arabidopsis MAP kinase 4 (MPK4) exists in nuclear complexes with the WRKY33 transcription factor. This complex depends on the MPK4 substrate MKS1. Challenge with Pseudomonas syringae or flagellin leads to the activation of MPK4 and phosphorylation of MKS1. Subsequently, complexes with MKS1 and WRKY33 are released from MPK4, and WRKY33 targets the promoter of PHYTOALEXIN DEFICIENT3 (PAD3) encoding an enzyme required for the synthesis of antimicrobial camalexin. Hence, wrky33 mutants are impaired in the accumulation of PAD3 mRNA and camalexin production upon infection. That WRKY33 is an effector of MPK4 is further supported by the suppression of PAD3 expression in mpk4-wrky33 double mutant backgrounds. Our data establish direct links between MPK4 and innate immunity and provide an example of how a plant MAP kinase can regulate gene expression by releasing transcription factors in the nucleus upon activation.
U2 - 10.1038/emboj.2008.147
DO - 10.1038/emboj.2008.147
M3 - Journal article
C2 - 18650934
VL - 27
SP - 2214
EP - 2221
JO - E M B O Journal
JF - E M B O Journal
SN - 0261-4189
IS - 16
ER -
ID: 21331828