Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis. / Pedersen, Lotte Bang; Murray, T; Popham, D L; Setlow, P.

In: Journal of Bacteriology, Vol. 180, No. 18, 1998, p. 4967-73.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pedersen, LB, Murray, T, Popham, DL & Setlow, P 1998, 'Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.', Journal of Bacteriology, vol. 180, no. 18, pp. 4967-73.

APA

Pedersen, L. B., Murray, T., Popham, D. L., & Setlow, P. (1998). Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis. Journal of Bacteriology, 180(18), 4967-73.

Vancouver

Pedersen LB, Murray T, Popham DL, Setlow P. Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis. Journal of Bacteriology. 1998;180(18):4967-73.

Author

Pedersen, Lotte Bang ; Murray, T ; Popham, D L ; Setlow, P. / Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis. In: Journal of Bacteriology. 1998 ; Vol. 180, No. 18. pp. 4967-73.

Bibtex

@article{44873ad0e07911dcbee902004c4f4f50,
title = "Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.",
abstract = "The pbp gene (renamed dacC), identified by the Bacillus subtilis genome sequencing project, encodes a putative 491-residue protein with sequence homology to low-molecular-weight penicillin-binding proteins. Use of a transcriptional dacC-lacZ fusion revealed that dacC expression (i) is initiated at the end of stationary phase; (ii) depends strongly on transcription factor sigmaH; and (iii) appears to be initiated from a promoter located immediately upstream of yoxA, a gene of unknown function located upstream of dacC on the B. subtilis chromosome. A B. subtilis dacC insertional mutant grew and sporulated identically to wild-type cells, and dacC and wild-type spores had the same heat resistance, cortex structure, and germination and outgrowth kinetics. Expression of dacC in Escherichia coli showed that this gene encodes an approximately 59-kDa membrane-associated penicillin-binding protein which is highly toxic when overexpressed. Udgivelsesdato: 1998-Sep",
author = "Pedersen, {Lotte Bang} and T Murray and Popham, {D L} and P Setlow",
note = "Keywords: Bacillus subtilis; Bacterial Proteins; Carrier Proteins; Escherichia coli; Genes, Bacterial; Hexosyltransferases; Lac Operon; Molecular Weight; Muramoylpentapeptide Carboxypeptidase; Mutation; Penicillin-Binding Proteins; Peptidyl Transferases; Transcription, Genetic",
year = "1998",
language = "English",
volume = "180",
pages = "4967--73",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "18",

}

RIS

TY - JOUR

T1 - Characterization of dacC, which encodes a new low-molecular-weight penicillin-binding protein in Bacillus subtilis.

AU - Pedersen, Lotte Bang

AU - Murray, T

AU - Popham, D L

AU - Setlow, P

N1 - Keywords: Bacillus subtilis; Bacterial Proteins; Carrier Proteins; Escherichia coli; Genes, Bacterial; Hexosyltransferases; Lac Operon; Molecular Weight; Muramoylpentapeptide Carboxypeptidase; Mutation; Penicillin-Binding Proteins; Peptidyl Transferases; Transcription, Genetic

PY - 1998

Y1 - 1998

N2 - The pbp gene (renamed dacC), identified by the Bacillus subtilis genome sequencing project, encodes a putative 491-residue protein with sequence homology to low-molecular-weight penicillin-binding proteins. Use of a transcriptional dacC-lacZ fusion revealed that dacC expression (i) is initiated at the end of stationary phase; (ii) depends strongly on transcription factor sigmaH; and (iii) appears to be initiated from a promoter located immediately upstream of yoxA, a gene of unknown function located upstream of dacC on the B. subtilis chromosome. A B. subtilis dacC insertional mutant grew and sporulated identically to wild-type cells, and dacC and wild-type spores had the same heat resistance, cortex structure, and germination and outgrowth kinetics. Expression of dacC in Escherichia coli showed that this gene encodes an approximately 59-kDa membrane-associated penicillin-binding protein which is highly toxic when overexpressed. Udgivelsesdato: 1998-Sep

AB - The pbp gene (renamed dacC), identified by the Bacillus subtilis genome sequencing project, encodes a putative 491-residue protein with sequence homology to low-molecular-weight penicillin-binding proteins. Use of a transcriptional dacC-lacZ fusion revealed that dacC expression (i) is initiated at the end of stationary phase; (ii) depends strongly on transcription factor sigmaH; and (iii) appears to be initiated from a promoter located immediately upstream of yoxA, a gene of unknown function located upstream of dacC on the B. subtilis chromosome. A B. subtilis dacC insertional mutant grew and sporulated identically to wild-type cells, and dacC and wild-type spores had the same heat resistance, cortex structure, and germination and outgrowth kinetics. Expression of dacC in Escherichia coli showed that this gene encodes an approximately 59-kDa membrane-associated penicillin-binding protein which is highly toxic when overexpressed. Udgivelsesdato: 1998-Sep

M3 - Journal article

C2 - 9733705

VL - 180

SP - 4967

EP - 4973

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 18

ER -

ID: 2830738