Convergent molecular evolution of thermogenesis and circadian rhythm in Arctic ruminants
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Convergent molecular evolution of thermogenesis and circadian rhythm in Arctic ruminants. / Li, Manman; Li, Xinmei; Wu, Zhipei; Zhang, Guanghui; Wang, Nini; Dou, Mingle; Liu, Shanlin; Yang, Chentao; Meng, Guanliang; Sun, Hailu; Hvilsom, Christina; Xie, Guoxiang; Li, Yang; Li, Zhuo Hui; Wang, Wei; Jiang, Yu; Heller, Rasmus; Wang, Yu.
In: Proceedings of the Royal Society B: Biological Sciences, Vol. 290, No. 1999, 20230538, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Convergent molecular evolution of thermogenesis and circadian rhythm in Arctic ruminants
AU - Li, Manman
AU - Li, Xinmei
AU - Wu, Zhipei
AU - Zhang, Guanghui
AU - Wang, Nini
AU - Dou, Mingle
AU - Liu, Shanlin
AU - Yang, Chentao
AU - Meng, Guanliang
AU - Sun, Hailu
AU - Hvilsom, Christina
AU - Xie, Guoxiang
AU - Li, Yang
AU - Li, Zhuo Hui
AU - Wang, Wei
AU - Jiang, Yu
AU - Heller, Rasmus
AU - Wang, Yu
PY - 2023
Y1 - 2023
N2 - The muskox and reindeer are the only ruminants that have evolved to survive in harsh Arctic environments. However, the genetic basis of this Arctic adaptation remains largely unclear. Here, we compared a de novo assembled muskox genome with reindeer and other ruminant genomes to identify convergent amino acid substitutions, rapidly evolving genes and positively selected genes among the two Arctic ruminants. We found these candidate genes were mainly involved in brown adipose tissue (BAT) thermogenesis and circadian rhythm. Furthermore, by integrating transcriptomic data from goat adipose tissues (white and brown), we demonstrated that muskox and reindeer may have evolved modulating mitochondrion, lipid metabolism and angiogenesis pathways to enhance BAT thermogenesis. In addition, results from co-immunoprecipitation experiments prove that convergent amino acid substitution of the angiogenesis-related gene hypoxia-inducible factor 2alpha (HIF2A), resulting in weakening of its interaction with prolyl hydroxylase domain-containing protein 2 (PHD2), may increase angiogenesis of BAT. Altogether, our work provides new insights into the molecular mechanisms involved in Arctic adaptation.
AB - The muskox and reindeer are the only ruminants that have evolved to survive in harsh Arctic environments. However, the genetic basis of this Arctic adaptation remains largely unclear. Here, we compared a de novo assembled muskox genome with reindeer and other ruminant genomes to identify convergent amino acid substitutions, rapidly evolving genes and positively selected genes among the two Arctic ruminants. We found these candidate genes were mainly involved in brown adipose tissue (BAT) thermogenesis and circadian rhythm. Furthermore, by integrating transcriptomic data from goat adipose tissues (white and brown), we demonstrated that muskox and reindeer may have evolved modulating mitochondrion, lipid metabolism and angiogenesis pathways to enhance BAT thermogenesis. In addition, results from co-immunoprecipitation experiments prove that convergent amino acid substitution of the angiogenesis-related gene hypoxia-inducible factor 2alpha (HIF2A), resulting in weakening of its interaction with prolyl hydroxylase domain-containing protein 2 (PHD2), may increase angiogenesis of BAT. Altogether, our work provides new insights into the molecular mechanisms involved in Arctic adaptation.
U2 - 10.1098/rspb.2023.0538
DO - 10.1098/rspb.2023.0538
M3 - Journal article
C2 - 37253422
VL - 290
JO - Proceedings of the Royal Society B: Biological Sciences
JF - Proceedings of the Royal Society B: Biological Sciences
SN - 0962-8452
IS - 1999
M1 - 20230538
ER -
ID: 356507438