Downregulation of the taurine transporter TauT during hypo-osmotic stress in NIH3T3 mouse fibroblasts

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Downregulation of the taurine transporter TauT during hypo-osmotic stress in NIH3T3 mouse fibroblasts. / Hansen, Daniel Bloch; Friis, Martin Barfred; Hoffmann, Else Kay; Lambert, Ian Henry.

In: Journal of Membrane Biology, Vol. 245, No. 2, 2012, p. 77-87.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, DB, Friis, MB, Hoffmann, EK & Lambert, IH 2012, 'Downregulation of the taurine transporter TauT during hypo-osmotic stress in NIH3T3 mouse fibroblasts', Journal of Membrane Biology, vol. 245, no. 2, pp. 77-87. https://doi.org/10.1007/s00232-012-9416-8

APA

Hansen, D. B., Friis, M. B., Hoffmann, E. K., & Lambert, I. H. (2012). Downregulation of the taurine transporter TauT during hypo-osmotic stress in NIH3T3 mouse fibroblasts. Journal of Membrane Biology, 245(2), 77-87. https://doi.org/10.1007/s00232-012-9416-8

Vancouver

Hansen DB, Friis MB, Hoffmann EK, Lambert IH. Downregulation of the taurine transporter TauT during hypo-osmotic stress in NIH3T3 mouse fibroblasts. Journal of Membrane Biology. 2012;245(2):77-87. https://doi.org/10.1007/s00232-012-9416-8

Author

Hansen, Daniel Bloch ; Friis, Martin Barfred ; Hoffmann, Else Kay ; Lambert, Ian Henry. / Downregulation of the taurine transporter TauT during hypo-osmotic stress in NIH3T3 mouse fibroblasts. In: Journal of Membrane Biology. 2012 ; Vol. 245, No. 2. pp. 77-87.

Bibtex

@article{edb23d46625b493293d7635d731d7919,
title = "Downregulation of the taurine transporter TauT during hypo-osmotic stress in NIH3T3 mouse fibroblasts",
abstract = "The present work was initiated to investigate regulation of the taurine transporter TauT by reactive oxygen species (ROS) and the tonicity-responsive enhancer binding protein (TonEBP) in NIH3T3 mouse fibroblasts during acute and long-term (4 h) exposure to low-sodium/hypo-osmotic stress. Taurine influx is reduced following reduction in osmolarity, keeping the extracellular Na(+) concentration constant. TonEBP activity is unaltered, whereas TauT transcription as well as TauT activity are significantly reduced under hypo-osmotic conditions. In contrast, TonEBP activity and TauT transcription are significantly increased following hyperosmotic exposure. Swelling-induced ROS production in NIH3T3 fibroblasts is generated by NOX4 and by increasing total ROS, by either exogenous application of H(2)O(2) or overexpressing NOX4, we demonstrate that TonEBP activity and taurine influx are regulated negatively by ROS under hypo-osmotic, low-sodium conditions, whereas the TauT mRNA level is unaffected. Acute exposure to ROS reduces taurine uptake as a result of modulated TauT transport kinetics. Thus, swelling-induced ROS production could account for the reduced taurine uptake under low-sodium/hypo-osmotic conditions by direct modulation of TauT.",
author = "Hansen, {Daniel Bloch} and Friis, {Martin Barfred} and Hoffmann, {Else Kay} and Lambert, {Ian Henry}",
year = "2012",
doi = "10.1007/s00232-012-9416-8",
language = "English",
volume = "245",
pages = "77--87",
journal = "Journal of Membrane Biology",
issn = "0022-2631",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Downregulation of the taurine transporter TauT during hypo-osmotic stress in NIH3T3 mouse fibroblasts

AU - Hansen, Daniel Bloch

AU - Friis, Martin Barfred

AU - Hoffmann, Else Kay

AU - Lambert, Ian Henry

PY - 2012

Y1 - 2012

N2 - The present work was initiated to investigate regulation of the taurine transporter TauT by reactive oxygen species (ROS) and the tonicity-responsive enhancer binding protein (TonEBP) in NIH3T3 mouse fibroblasts during acute and long-term (4 h) exposure to low-sodium/hypo-osmotic stress. Taurine influx is reduced following reduction in osmolarity, keeping the extracellular Na(+) concentration constant. TonEBP activity is unaltered, whereas TauT transcription as well as TauT activity are significantly reduced under hypo-osmotic conditions. In contrast, TonEBP activity and TauT transcription are significantly increased following hyperosmotic exposure. Swelling-induced ROS production in NIH3T3 fibroblasts is generated by NOX4 and by increasing total ROS, by either exogenous application of H(2)O(2) or overexpressing NOX4, we demonstrate that TonEBP activity and taurine influx are regulated negatively by ROS under hypo-osmotic, low-sodium conditions, whereas the TauT mRNA level is unaffected. Acute exposure to ROS reduces taurine uptake as a result of modulated TauT transport kinetics. Thus, swelling-induced ROS production could account for the reduced taurine uptake under low-sodium/hypo-osmotic conditions by direct modulation of TauT.

AB - The present work was initiated to investigate regulation of the taurine transporter TauT by reactive oxygen species (ROS) and the tonicity-responsive enhancer binding protein (TonEBP) in NIH3T3 mouse fibroblasts during acute and long-term (4 h) exposure to low-sodium/hypo-osmotic stress. Taurine influx is reduced following reduction in osmolarity, keeping the extracellular Na(+) concentration constant. TonEBP activity is unaltered, whereas TauT transcription as well as TauT activity are significantly reduced under hypo-osmotic conditions. In contrast, TonEBP activity and TauT transcription are significantly increased following hyperosmotic exposure. Swelling-induced ROS production in NIH3T3 fibroblasts is generated by NOX4 and by increasing total ROS, by either exogenous application of H(2)O(2) or overexpressing NOX4, we demonstrate that TonEBP activity and taurine influx are regulated negatively by ROS under hypo-osmotic, low-sodium conditions, whereas the TauT mRNA level is unaffected. Acute exposure to ROS reduces taurine uptake as a result of modulated TauT transport kinetics. Thus, swelling-induced ROS production could account for the reduced taurine uptake under low-sodium/hypo-osmotic conditions by direct modulation of TauT.

U2 - 10.1007/s00232-012-9416-8

DO - 10.1007/s00232-012-9416-8

M3 - Journal article

C2 - 22383044

VL - 245

SP - 77

EP - 87

JO - Journal of Membrane Biology

JF - Journal of Membrane Biology

SN - 0022-2631

IS - 2

ER -

ID: 37845299