E. coli dihydroorotate dehydrogenase reveals structural and functional distinctions between different classes of dihydroorotate dehydrogenases.
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E. coli dihydroorotate dehydrogenase reveals structural and functional distinctions between different classes of dihydroorotate dehydrogenases. / Nørager, Sofie; Jensen, Kaj Frank; Björnberg, Olof; Larsen, Sine.
In: Structure, Vol. 10, 2002, p. 1211-1223.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - E. coli dihydroorotate dehydrogenase reveals structural and functional distinctions between different classes of dihydroorotate dehydrogenases.
AU - Nørager, Sofie
AU - Jensen, Kaj Frank
AU - Björnberg, Olof
AU - Larsen, Sine
N1 - Author Keywords: flavoproteins; hydride transfer; pyrimidine nucleotide biosynthesis; reaction mechanism; structural comparisons; orotate binding
PY - 2002
Y1 - 2002
N2 - The flavoenzymes dihydroorotate dehydrogenases (DHODs) catalyze the fourth and only redox step in the de novo biosynthesis of UMP. Enzymes belonging to class 2, according to their amino acid sequence, are characterized by having a serine residue as the catalytic base and a longer N terminus. The structure of class 2 E. coli DHOD, determined by MAD phasing, showed that the N-terminal extension forms a separate domain. The catalytic serine residue has an environment differing from the equivalent cysteine in class 1 DHODs. Significant differences between the two classes of DHODs were identified by comparison of the E. coli DHOD with the other known DHOD structures, and differences with the class 2 human DHOD explain the variation in their inhibitors.
AB - The flavoenzymes dihydroorotate dehydrogenases (DHODs) catalyze the fourth and only redox step in the de novo biosynthesis of UMP. Enzymes belonging to class 2, according to their amino acid sequence, are characterized by having a serine residue as the catalytic base and a longer N terminus. The structure of class 2 E. coli DHOD, determined by MAD phasing, showed that the N-terminal extension forms a separate domain. The catalytic serine residue has an environment differing from the equivalent cysteine in class 1 DHODs. Significant differences between the two classes of DHODs were identified by comparison of the E. coli DHOD with the other known DHOD structures, and differences with the class 2 human DHOD explain the variation in their inhibitors.
U2 - 10.1016/S0969-2126(02)00831-6
DO - 10.1016/S0969-2126(02)00831-6
M3 - Journal article
VL - 10
SP - 1211
EP - 1223
JO - Structure
JF - Structure
SN - 0969-2126
ER -
ID: 6091143