Evolution of A bHLH Interaction Motif
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Evolution of A bHLH Interaction Motif. / Millard, Peter S.; Kragelund, Birthe B.; Burow, Meike.
In: International Journal of Molecular Sciences, Vol. 22, No. 1, 447, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Evolution of A bHLH Interaction Motif
AU - Millard, Peter S.
AU - Kragelund, Birthe B.
AU - Burow, Meike
PY - 2021
Y1 - 2021
N2 - Intrinsically disordered proteins and regions with their associated short linear motifs play key roles in transcriptional regulation. The disordered MYC-interaction motif (MIM) mediates interactions between MYC and MYB transcription factors in Arabidopsis thaliana that are critical for constitutive and induced glucosinolate (GLS) biosynthesis. GLSs comprise a class of plant defense compounds that evolved in the ancestor of the Brassicales order. We used a diverse set of search strategies to discover additional occurrences of the MIM in other proteins and in other organisms and evaluate the findings by means of structural predictions, interaction assays, and biophysical experiments. Our search revealed numerous MIM instances spread throughout the angiosperm lineage. Experiments verify that several of the newly discovered MIM-containing proteins interact with MYC TFs. Only hits found within the same transcription factor family and having similar characteristics could be validated, indicating that structural predictions and sequence similarity are good indicators of whether the presence of a MIM mediates interaction. The experimentally validated MIMs are found in organisms outside the Brassicales order, showing that MIM function is broader than regulating GLS biosynthesis.
AB - Intrinsically disordered proteins and regions with their associated short linear motifs play key roles in transcriptional regulation. The disordered MYC-interaction motif (MIM) mediates interactions between MYC and MYB transcription factors in Arabidopsis thaliana that are critical for constitutive and induced glucosinolate (GLS) biosynthesis. GLSs comprise a class of plant defense compounds that evolved in the ancestor of the Brassicales order. We used a diverse set of search strategies to discover additional occurrences of the MIM in other proteins and in other organisms and evaluate the findings by means of structural predictions, interaction assays, and biophysical experiments. Our search revealed numerous MIM instances spread throughout the angiosperm lineage. Experiments verify that several of the newly discovered MIM-containing proteins interact with MYC TFs. Only hits found within the same transcription factor family and having similar characteristics could be validated, indicating that structural predictions and sequence similarity are good indicators of whether the presence of a MIM mediates interaction. The experimentally validated MIMs are found in organisms outside the Brassicales order, showing that MIM function is broader than regulating GLS biosynthesis.
KW - MYB
KW - bHLH
KW - MYC
KW - transcription factor
KW - short linear motif (SLiM)
KW - intrinsically disordered protein (IDP)
KW - protein interaction
KW - interaction motif
KW - plant defense metabolism
KW - jasmonate signaling
U2 - 10.3390/ijms22010447
DO - 10.3390/ijms22010447
M3 - Journal article
C2 - 33466276
VL - 22
JO - International Journal of Molecular Sciences (Online)
JF - International Journal of Molecular Sciences (Online)
SN - 1661-6596
IS - 1
M1 - 447
ER -
ID: 255692230