Ex vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Ex vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents. / Rasmussen, Rikke H.; Jansen-Olesen, Inger; Kristensen, David M.; Christensen, Sarah L.
In: Journal of Visualized Experiments, Vol. 183, e63723, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Ex vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
AU - Rasmussen, Rikke H.
AU - Jansen-Olesen, Inger
AU - Kristensen, David M.
AU - Christensen, Sarah L.
PY - 2022
Y1 - 2022
N2 - Calcitonin gene-related peptide (CGRP) was first discovered in the 1980s as a splice variant from the calcitonin gene. Since its discovery, its role in migraine pathophysiology has been well established, first by its potent vasodilator properties and subsequently by its presence and function as a neurotransmitter in the sensory trigeminovascular system. The migraine-provoking ability of CGRP gave support to the pharma industry to develop monoclonal antibodies and antagonists inhibiting the effect of CGRP. A new treatment paradigm has proven effective in the prophylactic treatment of migraine. One of the useful tools to further understand migraine mechanisms is the ex vivo model of CGRP release from the trigeminovascular system. It is a relatively simple method that can be used with various pharmacological tools to achieve know-how to further develop new effective migraine treatments. The present protocol describes a CGRP release model and the technique to quantify the effect of pharmacological agents on the amount of CGRP released from the trigeminovascular system in rodents. A procedure describing the experimental approach from euthanasia to the measurement of protein levels is provided. The essential isolation of the trigeminal ganglion and the trigeminal nucleus caudalis from both mice and rats and the preparation of rat dura mater are described in detail. Furthermore, representative results from both species (rats and mice) are presented. The technique is a key tool to investigate the molecular mechanisms involved in migraine pathophysiology by using various pharmacological compounds and genetically modified animals.
AB - Calcitonin gene-related peptide (CGRP) was first discovered in the 1980s as a splice variant from the calcitonin gene. Since its discovery, its role in migraine pathophysiology has been well established, first by its potent vasodilator properties and subsequently by its presence and function as a neurotransmitter in the sensory trigeminovascular system. The migraine-provoking ability of CGRP gave support to the pharma industry to develop monoclonal antibodies and antagonists inhibiting the effect of CGRP. A new treatment paradigm has proven effective in the prophylactic treatment of migraine. One of the useful tools to further understand migraine mechanisms is the ex vivo model of CGRP release from the trigeminovascular system. It is a relatively simple method that can be used with various pharmacological tools to achieve know-how to further develop new effective migraine treatments. The present protocol describes a CGRP release model and the technique to quantify the effect of pharmacological agents on the amount of CGRP released from the trigeminovascular system in rodents. A procedure describing the experimental approach from euthanasia to the measurement of protein levels is provided. The essential isolation of the trigeminal ganglion and the trigeminal nucleus caudalis from both mice and rats and the preparation of rat dura mater are described in detail. Furthermore, representative results from both species (rats and mice) are presented. The technique is a key tool to investigate the molecular mechanisms involved in migraine pathophysiology by using various pharmacological compounds and genetically modified animals.
KW - SUPERIOR SAGITTAL SINUS
KW - MIDDLE MENINGEAL ARTERY
KW - NERVE-FIBERS
KW - SUBSTANCE-P
KW - EXTRACEREBRAL CIRCULATION
KW - PERIVASCULAR PEPTIDES
KW - CEREBRAL-ARTERIES
KW - CALCIUM-CHANNELS
KW - LIGHT AVERSION
KW - CGRP RECEPTOR
U2 - 10.3791/63723
DO - 10.3791/63723
M3 - Journal article
C2 - 35635478
VL - 183
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
SN - 1940-087X
M1 - e63723
ER -
ID: 312709456