Further genetic localization of the transforming sequences of the p21 v-ras gene of Harvey murine sarcoma virus.

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Further genetic localization of the transforming sequences of the p21 v-ras gene of Harvey murine sarcoma virus. / Willumsen, B M; Ellis, R W; Scolnick, E M; Lowy, D R.

In: Journal of Virology, Vol. 49, No. 2, 1984, p. 601-3.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Willumsen, BM, Ellis, RW, Scolnick, EM & Lowy, DR 1984, 'Further genetic localization of the transforming sequences of the p21 v-ras gene of Harvey murine sarcoma virus.', Journal of Virology, vol. 49, no. 2, pp. 601-3.

APA

Willumsen, B. M., Ellis, R. W., Scolnick, E. M., & Lowy, D. R. (1984). Further genetic localization of the transforming sequences of the p21 v-ras gene of Harvey murine sarcoma virus. Journal of Virology, 49(2), 601-3.

Vancouver

Willumsen BM, Ellis RW, Scolnick EM, Lowy DR. Further genetic localization of the transforming sequences of the p21 v-ras gene of Harvey murine sarcoma virus. Journal of Virology. 1984;49(2):601-3.

Author

Willumsen, B M ; Ellis, R W ; Scolnick, E M ; Lowy, D R. / Further genetic localization of the transforming sequences of the p21 v-ras gene of Harvey murine sarcoma virus. In: Journal of Virology. 1984 ; Vol. 49, No. 2. pp. 601-3.

Bibtex

@article{9a5e6180e3b311dcbee902004c4f4f50,
title = "Further genetic localization of the transforming sequences of the p21 v-ras gene of Harvey murine sarcoma virus.",
abstract = "The sequences encoding the 21-kilodalton transforming protein (p21 ras) of Harvey murine sarcoma virus have previously been localized genetically to a 1.3-kilobase segment of the viral DNA (E. H. Chang, R. W. Ellis, E. M. Scolnick, and D. R. Lowy, Science 210:1249-1251, 1980). Within this segment, DNA sequence analysis has found a single open reading frame large enough to encode the viral p21 (R. Dhar, R. W. Ellis, T. Y. Shih, S. Oroszlan, B. Shapiro, J. Maizel, D. Lowy, and E. M. Scolnick, Science 217:934-937, 1982). There are three potential in-frame ATG initiation codons at the 5' end of this open reading frame. By constructing a mutant of Harvey murine sarcoma virus DNA from which the first two ATG codons of this open reading frame have been deleted, we now show by transfection of the mutant viral DNA into NIH 3T3 cells that only the third ATG codon is necessary and sufficient for synthesis of the viral p21 and for cellular transformation.",
author = "Willumsen, {B M} and Ellis, {R W} and Scolnick, {E M} and Lowy, {D R}",
note = "Keywords: Animals; Base Sequence; Cell Transformation, Neoplastic; Cells, Cultured; Chromosome Deletion; Codon; DNA Restriction Enzymes; Genes; Genes, Viral; Harvey murine sarcoma virus; Mice; Mutation; Sarcoma Viruses, Murine; Transfection; Viral Proteins",
year = "1984",
language = "English",
volume = "49",
pages = "601--3",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "2",

}

RIS

TY - JOUR

T1 - Further genetic localization of the transforming sequences of the p21 v-ras gene of Harvey murine sarcoma virus.

AU - Willumsen, B M

AU - Ellis, R W

AU - Scolnick, E M

AU - Lowy, D R

N1 - Keywords: Animals; Base Sequence; Cell Transformation, Neoplastic; Cells, Cultured; Chromosome Deletion; Codon; DNA Restriction Enzymes; Genes; Genes, Viral; Harvey murine sarcoma virus; Mice; Mutation; Sarcoma Viruses, Murine; Transfection; Viral Proteins

PY - 1984

Y1 - 1984

N2 - The sequences encoding the 21-kilodalton transforming protein (p21 ras) of Harvey murine sarcoma virus have previously been localized genetically to a 1.3-kilobase segment of the viral DNA (E. H. Chang, R. W. Ellis, E. M. Scolnick, and D. R. Lowy, Science 210:1249-1251, 1980). Within this segment, DNA sequence analysis has found a single open reading frame large enough to encode the viral p21 (R. Dhar, R. W. Ellis, T. Y. Shih, S. Oroszlan, B. Shapiro, J. Maizel, D. Lowy, and E. M. Scolnick, Science 217:934-937, 1982). There are three potential in-frame ATG initiation codons at the 5' end of this open reading frame. By constructing a mutant of Harvey murine sarcoma virus DNA from which the first two ATG codons of this open reading frame have been deleted, we now show by transfection of the mutant viral DNA into NIH 3T3 cells that only the third ATG codon is necessary and sufficient for synthesis of the viral p21 and for cellular transformation.

AB - The sequences encoding the 21-kilodalton transforming protein (p21 ras) of Harvey murine sarcoma virus have previously been localized genetically to a 1.3-kilobase segment of the viral DNA (E. H. Chang, R. W. Ellis, E. M. Scolnick, and D. R. Lowy, Science 210:1249-1251, 1980). Within this segment, DNA sequence analysis has found a single open reading frame large enough to encode the viral p21 (R. Dhar, R. W. Ellis, T. Y. Shih, S. Oroszlan, B. Shapiro, J. Maizel, D. Lowy, and E. M. Scolnick, Science 217:934-937, 1982). There are three potential in-frame ATG initiation codons at the 5' end of this open reading frame. By constructing a mutant of Harvey murine sarcoma virus DNA from which the first two ATG codons of this open reading frame have been deleted, we now show by transfection of the mutant viral DNA into NIH 3T3 cells that only the third ATG codon is necessary and sufficient for synthesis of the viral p21 and for cellular transformation.

M3 - Journal article

C2 - 6319763

VL - 49

SP - 601

EP - 603

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 2

ER -

ID: 2890731